7TFP CDH1 mutant reporter
(Plasmid #91703)

• Purpose: lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes
• Depositing Lab: Bob Weinberg
• Publication: Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680.

Backbone

• Vector backbone: 7TFP
• Backbone manufacturer: Roel Nusse (Addgene plasmid # 24308)
• Backbone size w/o insert (bp): 9061
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDH1 mutant promoter
• Species: H. sapiens (human)
• Insert Size (bp): 243
• Mutation: mutant E-boxes
• Entrez Gene: CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
• Promoter: CDH1 mutant
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sbf1 (unknown if destroyed)
• 3′ cloning site: Bmt1 (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry