pCAGIG_V5-PPP6C
(Plasmid
#92013)
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PurposeExpresses V5-PPP6C, includes IRES-Emerald
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAGIG
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Backbone manufacturerConnie Cepko Lab (Addgene #11159)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPP6C
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SpeciesH. sapiens (human)
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Entrez GenePPP6C (a.k.a. PP6, PP6C)
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe coding sequence for PPP6C was obtained from the Invitrogen Ultimate ORF LITE Library (Clone ID IOH7224).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The fluorescent protein in this plasmid is mEmerald, but with F64 (a residue seen in unmodified GFP.)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGIG_V5-PPP6C was a gift from Joshua Mendell (Addgene plasmid # 92013 ; http://n2t.net/addgene:92013 ; RRID:Addgene_92013) -
For your References section:
An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT. Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23. 10.1038/nature21025 PubMed 28114302
