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VE-cad-eGFP Donor plasmid
(Plasmid #92309)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92309 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Oct4-2A-eGFP backbone
  • Backbone size w/o insert (bp) 8563
  • Total vector size (bp) 10438
  • Modifications to backbone
    Left and right arms of Oct4 were replaced with VEcad arms
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VEcad Homologous Recombination Arms
  • Alt name
    CDH5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    about 30 a.a. truncated at end of protein
  • GenBank ID
    1003
  • Entrez Gene
    CDH5 (a.k.a. 7B4, CD144)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI, AscI (not destroyed)
  • 3′ cloning site NheI, FseI (not destroyed)
  • 5′ sequencing primer M13 RVS
  • 3′ sequencing primer M13 FWD
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VE-cad-eGFP Donor plasmid was a gift from Sean Palecek (Addgene plasmid # 92309 ; http://n2t.net/addgene:92309 ; RRID:Addgene_92309)
  • For your References section:

    Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells. Bao X, Bhute VJ, Han T, Qian T, Lian X, Palecek SP. Bioeng. Transl. Med. (2017). 2: 191–201. 10.1002/btm2.10062