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pTK Cas9-2A-Citrine
(Plasmid #92393)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92393 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTK
  • Backbone size w/o insert (bp) 4157
  • Total vector size (bp) 9101
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 2A Citrine
  • Species
    S. pyogenes
  • Insert Size (bp)
    4944
  • Promoter thymidine kinase promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cctgcggctgatctatctgg
  • 3′ sequencing primer attcacttggggcatgctca
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTK EGFP was made by Uchikawa et al 2003, by insertion of the Herpes simplex virus thymidine kinase promoter in the polylinker HindIII site of pCAT3-basic vector (Promega), and by replacing the CAT gene with the EGFP gene (Clontech). We have modified this vector by inserting BsmBI flanked lacZ MCS cassette and replaced EGFP with Citrine. Subsequently we amplified Cas9 from #42230 and inserted this followed by a 2A sequence into the NcoI site in the modified pTK citrine vector.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718

Functional analysis of chicken Sox2 enhancers highlights an array of diverse regulatory elements that are conserved in mammals. Uchikawa M1, Ishida Y, Takemoto T, Kamachi Y, Kondoh H. Dev Cell. 2003 Apr;4(4):509-19.

Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK Cas9-2A-Citrine was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 92393 ; http://n2t.net/addgene:92393 ; RRID:Addgene_92393)
  • For your References section:

    Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. 10.1242/dev.160333 PubMed 29386245