pLenti_CamKIIa_mKate2
(Plasmid #96942)

• Purpose: To label mature excitatory iPSC-derived human neurons
• Depositing Labs: Ricardo Dolmetsch, Oleksandr Shcheglovitov
• Publication: Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16.

Backbone

• Vector backbone: pLenti
• Vector type: Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: far-red fluorescent protein
• Alt name: mKate2
• Mutation: E87D (please see depositor comments below)
• Promoter: CaMKIIa

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CCGGTGCCACCATGGTGA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation E87D in mKate2 was found during Addgene's quality control process. The depositor noted that this mutation does NOT affect plasmid function.