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Addgene

pEff Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extend ladder and a digestion of the plasmid pEff with the following enzymes: NdeI and AscI.

Digested with NdeI, expected band at 14.5 kb.
Digested with AscI, expected band at 14.5 kb.
Digested with NdeI/AscI, expected bands at 8.6 and 5.9 kb.

These results display the expected band sizes.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Extend Ladder and a digestion of the plasmid pEff with the following enzymes: SmaI and AgeI-HF