ZAK (ZAKA-c057)
(Plasmid
#98225)
-
PurposeBaculovirus expression for structure determination; may not be full ORF
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepFB-LIC-Bse
-
Backbone manufacturerOpher Gileadi (Addgene plasmid # 26108)
-
Vector typeBaculovirus
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameZAKA
-
Alt nameZAK
-
Alt namesterile-alpha motif and leucine zipper containing kinase AZK
-
SpeciesH. sapiens (human)
-
Mutationpfam00069:Pkinase 16-257
-
Entrez GeneMAP3K20 (a.k.a. AZK, CNM6, MLK7, MLT, MLTK, MLTKalpha, MLTKbeta, MRK, SFMMP, ZAK, mlklak, pk)
- Promoter polyhedrin
-
Tag
/ Fusion Protein
- His-6-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Fbac-1
- 3′ sequencing primer Fbac-2 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. 5HES: http://www.thesgc.org/structures/5HES
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ZAK (ZAKA-c057) was a gift from Nicola Burgess-Brown (Addgene plasmid # 98225 ; http://n2t.net/addgene:98225 ; RRID:Addgene_98225)
