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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2
(Plasmid #98973)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98973 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pX460
  • Backbone manufacturer
    Feng Zhang
  • Total vector size (bp) 6815
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LMNA sgRNAs pair 2 and Cas9(D10A)
  • Species
    H. sapiens (human)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2 was a gift from Jan Karlseder (Addgene plasmid # 98973 ; http://n2t.net/addgene:98973 ; RRID:Addgene_98973)
  • For your References section:

    Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J. Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. 10.1038/nature24023 PubMed 28959974