HisGB1-Hif1a(776-826)_FIH
(Plasmid #99508)

• Purpose: Bacterial expression of hydroxylated Hif1a TAD (776-826)
• Depositing Lab: Peter Wright
• Publication: Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-15. Epub 2007 Nov 1.

Backbone

• Vector backbone: pET22b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5493
• Total vector size (bp): 7003
• Modifications to backbone: introduced second RBS
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Hif1a (776-826)
• Alt name: Hif1a
• Species: H. sapiens (human)
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Promoter: T7
• Tag / Fusion Protein:
  • His6GB1 (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: FIH1
• Alt name: HIF1AN
• Species: H. sapiens (human)
• GenBank ID: NM_017902
• Entrez Gene: HIF1AN (a.k.a. FIH1)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry