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Benjamin Ebert Lab Plasmids


The Benjamin Ebert Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
57818pL-CRISPR.EFS.GFPSpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-eGFP (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven pLKO.005 Add to Cart
57819pL-CRISPR.EFS.tRFPSpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-tRFP (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven pLKO.005 Add to Cart
57821pLKO5d.EFS.SpCas9.P2A.BSDSpCas9 (Other), EFS (Homo sapiens), P2A-BSD (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven pLKO.005 Add to Cart
57822pLKO5.sgRNA.EFS.GFPsgRNA, EFS, eGFP (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven pLKO.005 Add to Cart
57823pLKO5.sgRNA.EFS.tRFPsgRNA, EFS, tagRFP (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. 3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven pLKO.005 Add to Cart
57824pLKO5.sgRNA.EFS.tRFP657sgRNA, EFS, tagRFP657 (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven pLKO.005 Add to Cart
57825pLKO5.sgRNA.EFS.PACsgRNA, EFS, PAC (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven pLKO.005 Add to Cart
57826pL-CRISPR.SFFV.tRFPSpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-tRFP (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven pLKO.005 Add to Cart
57827pL-CRISPR.SFFV.GFPSpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-eGFP (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven pLKO.005 Add to Cart
57828pL-CRISPR.EFS.PACSpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-PAC (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven pLKO.005 Add to Cart
57829pL-CRISPR.SFFV.PACSpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-PAC (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven pLKO.005 Add to Cart
58329pLKO5d.EFS.SpCas9.P2A.PACSpCas9 (Other), EFS (Homo sapiens), P2A-PAC (Synthetic)Lentiviral, CRISPRGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven pLKO.005 Add to Cart
61395pL-SFFV.Reporter.RFP657.PACMammalian Expression, LentiviralGeneration of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance. RRL.PPT.SFFV.RFP657.IRES2.PAC.PRE Add to Cart