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Benoit Bruneau Lab Plasmids


The Benoit Bruneau Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Bruneau Lab Plasmids (unpublished)
Baf60c is essential for function of BAF chromatin remodelling complexes in heart development. Lickert H, Takeuchi JK, Von Both I, Walls JR, McAuliffe F, Adamson SL, Henkelman RM, Wrana JL, Rossant J, Bruneau BG Nature. 2004 Nov 4. 432(7013):107-12.
Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo. Agarwal P, Wylie JN, Galceran J, Arkhitko O, Li C, Deng C, Grosschedl R, Bruneau BG Development. 2003 Feb . 130(3):623-33.
The homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient. Costantini DL, Arruda EP, Agarwal P, Kim KH, Zhu Y, Zhu W, Lebel M, Cheng CW, Park CY, Pierce SA, Guerchicoff A, Pollevick GD, Chan TY, Kabir MG, Cheng SH, Husain M, Antzelevitch C, Srivastava D, Gross GJ, Hui CC, Backx PH, Bruneau BG Cell. 2005 Oct 21. 123(2):347-58.
Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
24715pCRII Topo-Tbx20 (14)Tbx20 (Mus musculus)Mammalian Expression Bruneau Lab Plasmids (unpublished) pCRII-TOPO Tbx20 9430010M06Rik, AL022859, Tbx12 Add to Cart
24723pCMV-Sport6 Baf60bBaf60b (Mus musculus)Mammalian Expression, Bacterial ExpressionBaf60c is essential for function of BAF chromatin remodelling complexes in heart development. Nature. 2004 Nov 4. 432(7013):107-12. pCMV-Sport6 Smarcd2 Baf60b, AW322457 Add to Cart
24994FGF10-luciferaseFgf10 promoter (Mus musculus)Mammalian Expression, LuciferaseTbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo. Development. 2003 Feb . 130(3):623-33. pXP1 Fgf10 AEY17, BB213776, Fgf-10, Gsfaey17 Add to Cart
24995pCMV5b HA Irx5 deltaIrotIrx5 (Mus musculus)Mammalian ExpressionThe homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient. Cell. 2005 Oct 21. 123(2):347-58. pCMV5b Irx5 Add to Cart
24996pCMV5b HA Irx5 deltaCIrx5 (Mus musculus)Mammalian ExpressionThe homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient. Cell. 2005 Oct 21. 123(2):347-58. pCMV5b Irx5 Add to Cart
24997pCMV5b HA Irx5 deltaHDIrx5 (Mus musculus)Mammalian ExpressionThe homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient. Cell. 2005 Oct 21. 123(2):347-58. pCMV5b Irx5 Add to Cart
86230pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting constructAID[71-114]-eGFP (Mus musculus)Mammalian Expression, Mouse TargetingTargeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system. pFNF (Addgene #22687) Add to Cart
86231pX335-EN475spCas9-nickase and sgRNA against mouse CTCF STOP Codon (Mus musculus)Mammalian Expression, Mouse TargetingTargeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477. pX335 Add to Cart
86232pX335-EN477spCas9-nickase and sgRNA against mouse CTCF STOP Codon (Mus musculus)Mammalian Expression, Mouse TargetingTargeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475. pX335 Add to Cart
86233pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26osTir1 (Homo sapiens)Mammalian Expression, Mouse TargetingTargeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system. pFNF (Addgene #22687) Add to Cart
86234pX330-EN479spCas9-nickase and sgRNA against mouse rosa26 locus (Mus musculus)Mammalian Expression, Mouse TargetingTargeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus pX330 Add to Cart