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Bo Huang Lab Plasmids


The Bo Huang Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
Versatile protein tagging in cells with split fluorescent protein. Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
51023pSLQ1658-dCas9-EGFPdCas9 fuse to EGFP (Homo sapiens)Mammalian Expression, Retroviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system) pMSCVpuro Add to Cart
51024pSLQ1651-sgTelomere(F+E)Optimized sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres pSico Add to Cart
51025pSLQ1661-sgMUC4-E3(F+E)optimized sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3 pSico MUC4 ASGP, HSA276359, MUC-4 Add to Cart
70217pEGFP-GFP11-Clathrin light chainGFP11-Clathrin light chain (Homo sapiens)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP11-clathrin light chain in mammalian cells pEGFP-mEmerald-clathrin-15 CLTA LCA Add to Cart
70218pACUH-GFP11x7-mCherry-α-tubulinGFP11x7-mCherry-α-tubulin (Drosophila melanogaster)Insect ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths. pACUH Add to Cart
70219pcDNA3.1-GFP(1-10)GFP(1-10) (Synthetic)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP(1-10) in mammalian cells pcDNA3.1(+) Add to Cart
70220pEGFP-sfCherry11x4-ß-actinsfCherry11x4-actin (Homo sapiens)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses sfCherry11x4-ß-actin in mammalian cells pEGFP-mEmerald-actin-C-18 Add to Cart
70221pEGFP-sfCherry11-ß-actinsfCherry11-actin (Homo sapiens)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses sfCherry11-ß-actin in mammalian cells pEGFP-mEmerald-actin-C-18 Add to Cart
70222pcDNA3.1-sfCherry(1-10)sfCherry(1-10) (Synthetic)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses sfCherry(1-10) in mammalian cells pcDNA3.1(+) Add to Cart
70224pHRm-NLS-dCas9-GFP11x7-NLS-P2A-BFP-NLSNLS-dCas9-GFP11x7-NLS-P2A-BFP-NLS (Synthetic)Mammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses NLS-dCas9-GFP11x7-NLS and BFP-NLS in mammalian cells pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE (addgene #60910) Add to Cart
70228pHRm-GFP(1-10)-VP64-NLSGFP(1-10)-VP64-NLSMammalian ExpressionVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP(1-10)-VP64-NLS in mammalian cells pHRm Add to Cart
80409pHR-SFFV-GFP1-10GFP1-10 (Synthetic)Mammalian Expression, LentiviralVersatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses GFP1-10 in mammalian cells pHR-SFFV Add to Cart
82602pcDNA3.1_sfCherry+(1-10)sfCherry+(1-10) (Synthetic)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+(1-10) in mammalian cells pcDNA3.1(+) Add to Cart
82603pSFFV_sfCherry+(1-10)sfCherry+(1-10) (Synthetic)Mammalian Expression, Lentiviral Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Lentiviral vector of constitutive expression of sfCherry+(1-10) pHR-SFFV Add to Cart
82604pEGFP_sfCherry+11_KeratinsfCherry+11_keratin (Homo sapiens)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+11 tagged keratin in mammalian cells pEGFP-mEmerald-Keratin14-N-18 Add to Cart
82605pEGFP_sfCherry+11_H2BsfCherry+11_H2B (Homo sapiens)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+11 tagged H2B in mammalian cells pEGFP-mEmerald-H2B Add to Cart
82606pSFFV_sfCherry+11_TagBFPsfCherry+11_TagBFP (Synthetic)Mammalian Expression, Lentiviral Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+11 and TagBFP fusion in mammalian cells pHR-SFFV Add to Cart
82607pEGFP_sfCherry+11_HP1sfCherry+11_HP1b (Homo sapiens)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+11 tagged heterochromatin protein1 in mammalian cells pEGFP-mEmerald-HP1b Add to Cart
82612pcDNA3.1-PACherry+(1-10)PACherry+(1-10) (Synthetic)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses PACherry+(1-10) in mammalian cells pcDNA3.1(+) Add to Cart
83030pET_sfCherry1-10_32aalinker_sfCherry11sfCherry1-10_32aalinker_sfCherry11 (Synthetic)Bacterial Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Bacterial expression vector for split sfCherry screening pET28a Add to Cart
83031pSFFV_sfCherry+FL_TagBFPsfCherry+full length_TagBFP (Synthetic)Mammalian Expression, Lentiviral Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+FL and TagBFP fusion in mammalian cells pHR-SFFV Add to Cart
83032pEGFP_sfCherry+11_Clathrin light chainsfCherry+11-Clathrin light chain (Homo sapiens)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+11 tagged clathrin light chain in mammalian cells pEGFP-mEmerald-clathrin-15 CLTA LCA Add to Cart
83033pEGFP_TOMM20_sfCherry+11TOMM20_sfCherry+11 (Homo sapiens)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+11 tagged TOMM20(human) in mammalian cells pEGFP-mEmerald-TOMM20-N-10 Add to Cart
83034pEGFP_sfCherry+11_zyxinTOMM20_sfCherry+11 (Synthetic)Mammalian Expression Improving Brightness of Red and Green Split Fluorescent Proteins for Tagging Endogenous Proteins (unpublished) Expresses sfCherry+11 tagged zyxin(human) in mammalian cells pEGFP-mEmerald-Zyxin-6 Add to Cart