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Charles Gersbach Lab Plasmids


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Title Authors Publication
Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.
Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. Polstein LR, Gersbach CA J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. Epub 2012 Sep 27.
RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, Crawford GE, Hartemink AJ, Gersbach CA Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 2013 Feb 3.
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA Nucleic Acids Res. 2014 Aug 13. pii: gku749.
Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak F, Reddy TE, Gersbach CA ACS Synth Biol. 2014 Dec 10.
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Polstein LR, Gersbach CA Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, Sanchez-Adams J, Gersbach CA, Guilak F Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 10.1073/pnas.1321744111. Epub 2014 Feb 18.
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
Single-molecule analysis of myocyte differentiation reveals bimodal lineage commitment. Gibson TM, Gersbach CA Integr Biol (Camb). 2015 Jun 8;7(6):663-71. doi: 10.1039/c5ib00057b. Epub 2015 May 8.
Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
37198pCMV-RosaL6 ELD mutationsFokI (fused to Rosa L6 ZFN)Mammalian Expression ; ZFN expressionGene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14. pCMV
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • SV40 NLS (N terminal on backbone)
    • ROSA26 eZFNs L6 (N terminal on insert)
  • Add to Cart
    37199pCMV-RosaR4 KKR mutationsFokI (fused to RosaR4 ZFN)Mammalian Expression ; ZFN expressionGene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14. pCMV Add to Cart
    37200pDonor MCS Rosa26Rosa26 left targeting arm (Mus musculus), MCS, Rosa26 right targeting armGenomic targeting - zinc fingerGene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14. pZDonor AAVS1 Add to Cart
    42214pGL3-Basic-9xSeq2-Luc9xSeq2 Binding SitesMammalian ExpressionLight-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. Epub 2012 Sep 27. pGL3-Basic Add to Cart
    42215pcDNA3-GI-ZFP2GIGANTEA-FLAG-ZFP2 (Arabidopsis thaliana)Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. Epub 2012 Sep 27. pcDNA3 Add to Cart
    47106pcDNA-dCas9dCas9 (Other)Mammalian Expression, CRISPRRNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells pcDNA3.1 Add to Cart
    47107pcDNA-dCas9-VP64dCas9-VP64 (Other)Mammalian Expression, CRISPRRNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells pcDNA3.1 Add to Cart
    47108pSPgRNASPgRNA (Synthetic)Mammalian Expression, CRISPRRNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells pZDonor Add to Cart
    47388pcDNA3.1-GoldenGateMammalian Expression, TALENSynergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 2013 Feb 3. Expression plasmid for TALE proteins compatible with GoldenGate cloning system pcDNA3.1
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • SV40 NLS (N terminal on insert)
    • HA (C terminal on insert)
    • SV40 NLS (C terminal on insert)
  • Add to Cart
    47389pcDNA3.1-GoldenGate-VP64Mammalian Expression, TALENSynergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 2013 Feb 3. Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system pcDNA3.1
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • SV40 NLS (N terminal on insert)
    • HA (C terminal on insert)
    • SV40 NLS (C terminal on insert)
  • Add to Cart
    52580pLVE-hTGFB3-IRES-Redhuman transforming growth factor-beta3 (Homo sapiens)Mammalian Expression, LentiviralScaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 10.1073/pnas.1321744111. Epub 2014 Feb 18. Lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells pLV-tTRKRAB-red TGFB3 ARVD, ARVD1, LDS5, RNHF, TGF-beta3 Add to Cart
    52581pLVE-eGFPeGFPMammalian Expression, LentiviralScaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 10.1073/pnas.1321744111. Epub 2014 Feb 18. Lentiviral transfer vector driving expression of eGFP in mammalian cells pLVU-tTR-KRAB Add to Cart
    53186phH1-gRNAMammalian Expression, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Expresses the S. pyogenes sgRNA from the H1 promoter pzDonor Add to Cart
    53187pmU6-gRNAMammalian Expression, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Expresses the S. pyogenes sgRNA from the mouse U6 promoter pzDonor Add to Cart
    53188phU6-gRNAMammalian Expression, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Expresses the S. pyogenes sgRNA from the human U6 promoter pzDonor Add to Cart
    53189ph7SK-gRNAMammalian Expression, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Expresses the S. pyogenes sgRNA from the 7SK promoter pzDonor Add to Cart
    53190pLV hUbC-Cas9-T2A-GFPhumanized Cas9 T2A GFP (Other)Mammalian Expression, Lentiviral, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. 3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP FUGW Add to Cart
    53191pLV hUbC-dCas9-T2A-GFPhumanized dead Cas9 T2A GFP (Other)Mammalian Expression, Lentiviral, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Co-expresses human optimized S. pyogenes dCas9 and GFP FUGW Add to Cart
    53192pLV hUbC-dCas9 VP64-T2A-GFPhumanized dead Cas9 VP64 T2A GFP (Other)Mammalian Expression, Lentiviral, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP FUGW Add to Cart
    59791pLV hUbC-VP64 dCas9 VP64-T2A-GFPhumanized VP64 dead Cas9 VP64 T2A GFP (Other)Mammalian Expression, Lentiviral, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP FUGW Add to Cart
    60551pcDNA3.1-CibN-dCas9CibN-dCas9 (Arabidopsis thaliana)Mammalian Expression, CRISPRA light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses CibN-dCas9 in mammalian cells pcDNA3.1 Add to Cart
    60552pcDNA3.1-dCas9-CibNdCas9-CibN (Arabidopsis thaliana)Mammalian Expression, CRISPRA light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses dCas9-CibN in mammalian cells pcDNA3.1 Add to Cart
    60553pcDNA3.1-CibN-dCas9-CibNdCas9-CibN (Arabidopsis thaliana)Mammalian Expression, CRISPRA light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses CibN-dCas9-CibN in mammalian cells pcDNA3.1 Add to Cart
    60554pcDNA3.1-Cry2FL-VP64Cry2FL-VP64 (Arabidopsis thaliana)Mammalian ExpressionA light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses Cry2FL-VP64 in mammalian cells pcDNA3.1 Add to Cart
    60622LV-Floxed LucFloxed LuciferaseLentiviral, Cre/Lox, LuciferaseEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses a conditional luciferase cassette dependent on Cre mediated recombination FUGW Add to Cart
    60623LV-TRE-Empty-T2A-dsRedExpress2EmptyLentiviralEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses dsRedExpress2 in response to doxycycline pRRL Add to Cart
    60624LV-TRE-WT mouse MyoD-T2A-dsRedExpress2WT mouse MyoD (Mus musculus)LentiviralEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses WT mouse MyoD and dsRedExpress2 in response to doxycycline pRRL Add to Cart
    60625LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2VP64 mouse MyoD (Mus musculus)LentiviralEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses VP64 mouse MyoD and dsRedExpress2 in response to doxycycline pRRL Add to Cart
    60626LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2VP16 mouse MyoD (Mus musculus)LentiviralEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline pRRL Add to Cart
    60627LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2p65 mouse MyoD (Mus musculus)LentiviralEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses p65 mouse MyoD and dsRedExpress2 in response to doxycycline pRRL Add to Cart
    60628LV-TRE-WT human MyoD-T2A-dsRedExpress2VP64 mouse MyoD (Homo sapiens)LentiviralEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses WT human MyoD and dsRedExpress2 in response to doxycycline pRRL MYOD1 MYF3, MYOD, PUM, bHLHc1 Add to Cart
    60629LV-TRE-VP64 human MyoD-T2A-dsRedExpress2VP64 human MyoD (Homo sapiens)LentiviralEnhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain. ACS Synth Biol. 2014 Dec 10. Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline pRRL Add to Cart
    60718pGL3-Basic-8x-gRNA-eGFP8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation pGL3-Basic Add to Cart
    60719gRNA-eGFP-ReportergRNA (5'-AAAGGTCGAGAAACTGCAAA-3')Mammalian Expression, CRISPRA light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter. pzDonor Add to Cart
    61355pcDNA-dCas9-HAS.pyogenes dCas9 with c-terminal HA tag (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter pcDNA3.1 Add to Cart
    61356pcDNA-dCas9-FLp300S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61357pcDNA-dCas9-p300 CoreS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61358pcDNA-dCas9-p300 Core (D1399Y)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61359pcDNA-dCas9-p300 Core (1645/1646 RR/EE)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61361pcDNA-dCas9-p300 Core (C1204R)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61362pcDNA-dCas9-p300 Core (Y1467F)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61363pcDNA-dCas9-p300 Core (1396/1397 SY/WW)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61364pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300 Add to Cart
    61365pcDNA3.3-Nm-dCas9-p300 CoreNm-dCas9-p300 Core (Homo sapiens)Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter pcDNA3.3 EP300 KAT3B, RSTS2, p300 Add to Cart
    61366pZDonor-Nm-Cas9-gRNA-hU6Mammalian Expression, CRISPR, Synthetic BiologyEpigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA pZdonor Add to Cart
    61441LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro MyoD (Mus musculus)LentiviralSingle-molecule analysis of myocyte differentiation reveals bimodal lineage commitment. Integr Biol (Camb). 2015 Jun 8;7(6):663-71. doi: 10.1039/c5ib00057b. Epub 2015 May 8. Dox-inducible MyoD with mRNA tag for smFISH pRRL Myod1 AI503393, MYF3, MyoD, Myod-1, bHLHc1 Add to Cart
    71236pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Purohumanized dCas9-KRAB T2A Puro (Other), sgRNALentiviral, CRISPRHighly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26. Express sgRNA and dCas9-KRAB from lentiviral vector FUGW Add to Cart
    71237pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFPhumanized dCas9-KRAB T2A GFP (Other), sgRNALentiviral, CRISPRHighly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26. Express sgRNA and dCas9-KRAB from lentiviral vector FUGW Add to Cart
    83889pLV-dCas9-p300-P2A-PuroRS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens), pac from Streptomyces alboniger (Other)Mammalian Expression, Lentiviral, CRISPRCRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. Lentiviral Sp dCas9-p300-P2A-PuroR lentiCRISPR v2 EP300 KAT3B, RSTS2, p300 Add to Cart
    83890pLV-dCas9-KRAB-PGK-HygRhumanized dead Cas9 KRAB (Other), aminoglycoside phosphotransferase from E. coli (Other)Lentiviral, CRISPRCRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette. FUGW Add to Cart
    83919pLV-U6-gRNA-UbC-DsRed-P2A-BsrDsRed-Express2, BsrLentiviral, CRISPRCRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR FUGW Add to Cart
    83925pLV-U6-gRNA-UbC-eGFP-P2A-BsreGFP, BsrLentiviral, CRISPRCRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR FUGW Add to Cart
    84034pLV GG hUbC-dsREDMammalian Expression, Lentiviral, CRISPRMultiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Contains Golden Gate cassette to express up to four gRNAs and dsRED FUGW Add to Cart