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Cynthia Wolberger Lab Plasmids


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Title Authors Publication
Structure of a Sir2 enzyme bound to an acetylated p53 peptide. Avalos JL, Celic I, Muhammad S, Cosgrove MS, Boeke JD, Wolberger C Mol Cell 2002 Sep;10(3):523-35.
Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera. Ke A, Wolberger C Protein Sci 2003 Feb;12(2):306-12.
Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. LaRonde-LeBlanc NA, Wolberger C Genes Dev 2003 Aug 15;17(16):2060-72.
The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Batchelor AH, Piper DE, de la Brousse FC, McKnight SL, Wolberger C Science 1998 Feb 13;279(5353):1037-41.
Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C Nat Struct Mol Biol. 2006 Oct . 13(10):915-20.
Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C Nat Struct Mol Biol. 2006 Jul . 13(7):661-2.
Mechanism of Sirtuin Inhibition by Nicotinamide: Altering the NAD(+) Cosubstrate Specificity of a Sir2 Enzyme. Avalos JL, Bever KM, Wolberger C Mol Cell 2005 Mar 18;17(6):855-68.
Structural and functional studies of the Rap1 C-terminus reveal novel separation-of-function mutants. Feeser EA, Wolberger C J Mol Biol. 2008 Jul 11. 380(3):520-31.
Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1. Wang T, Yin L, Cooper EM, Lai MY, Dickey S, Pickart CM, Fushman D, Wilkinson KD, Cohen RE, Wolberger C J Mol Biol. 2009 Mar 6. 386(4):1011-23.
Structural insights into the assembly and function of the SAGA deubiquitinating module. Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, Wolberger C Science. 2010 May 21. 328(5981):1025-9.
A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Berndsen CE, Wolberger C Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2.
The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R, Zhang X, Wang T, Wolberger C Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911.
E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, Wolberger C Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18.
Structural basis for histone H2B deubiquitination by the SAGA DUB module. Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681.
Side chain specificity of ADP-ribosylation by a sirtuin. Fahie K, Hu P, Swatkoski S, Cotter RJ, Zhang Y, Wolberger C FEBS J. 2009 Dec;276(23):7159-76. doi: 10.1111/j.1742-4658.2009.07427.x. Epub 2009 Nov 6.
The crystal structure of the tumor suppressor protein pp32 (Anp32a): structural insights into Anp32 family of proteins. Huyton T, Wolberger C Protein Sci. 2007 Jul;16(7):1308-15. Epub 2007 Jun 13.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
8380pProEX-Af2Sir2Sir2-Af2 full lengthBacterial ExpressionStructure of a Sir2 enzyme bound to an acetylated p53 peptide. Mol Cell 2002 Sep;10(3):523-35. pProEXHT SIRT2 SIR2, SIR2L, SIR2L2 Sir2.2 SIRT1 SIR2, SIR2L1, SIR2alpha Add to Cart
8383PET11a-Af2sir2Sir2-Af2 full lengthBacterial ExpressionStructure of a Sir2 enzyme bound to an acetylated p53 peptide. Mol Cell 2002 Sep;10(3):523-35. PET11a SIRT2 SIR2, SIR2L, SIR2L2 Sir2.2 SIRT1 SIR2, SIR2L1, SIR2alpha Add to Cart
8436pMal-MATa1MATa1(78aa-126aa) (Saccharomyces cerevisiae)Bacterial ExpressionInsights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera. Protein Sci 2003 Feb;12(2):306-12. modified pMal-c2x Add to Cart
8588pA9HDHoxA9 190-271with hexapeptide (Homo sapiens)Bacterial ExpressionStructure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. Genes Dev 2003 Aug 15;17(16):2060-72. PET28a+ HOXA9 ABD-B, HOX1, HOX1.7, HOX1G Add to Cart
8589pPBX1-318Human pbx (1-318) (Homo sapiens)Bacterial ExpressionStructure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. Genes Dev 2003 Aug 15;17(16):2060-72. PET21b PBX1 Add to Cart
8813PBB-79GABPb1 (1-157) (Mus musculus)Bacterial ExpressionThe structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Science 1998 Feb 13;279(5353):1037-41. pG5' Gabpb1 E4TF1, GABPB, BABPB2, E4Tf1B, NRF2B1, NRF2B2, E4TF1-47, E4TF1-53, GABPB1-1, GABPB1-2 Add to Cart
8814pBB-80GABPa1 (318-430) (Mus musculus)Bacterial ExpressionThe structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Science 1998 Feb 13;279(5353):1037-41. pG5' Gabpa GABPalpha Add to Cart
25464UBC13-PGEX4TUbc13 (Saccharomyces cerevisiae)Bacterial ExpressionMms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. pGEX4T UBC13 YDR092W MMS2 YGL087C Add to Cart
25465MBP-His6-TEV-MMS2MMS2 (Saccharomyces cerevisiae)Bacterial ExpressionMms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. pMalC2XHV MMS2 YGL087C UBC13 YDR092W Add to Cart
25630Nampt-PET28aNampt (Mus musculus)Bacterial ExpressionStructure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Nat Struct Mol Biol. 2006 Jul . 13(7):661-2. PET28a Nampt 1110035O14Rik, AI314458, AI480535, NAmPRTase, Pbef, Pbef1, Visfatin Add to Cart
25815TmSir2Tm Sir2Bacterial ExpressionMechanism of Sirtuin Inhibition by Nicotinamide: Altering the NAD(+) Cosubstrate Specificity of a Sir2 Enzyme. Mol Cell 2005 Mar 18;17(6):855-68. PET11a Add to Cart
25817pMal672HRap1(672-827) (Saccharomyces cerevisiae)Bacterial ExpressionStructural and functional studies of the Rap1 C-terminus reveal novel separation-of-function mutants. J Mol Biol. 2008 Jul 11. 380(3):520-31. pDEST 14 Add to Cart
26959hOtub1Otub1 (Homo sapiens)Bacterial ExpressionEvidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1. J Mol Biol. 2009 Mar 6. 386(4):1011-23. pProEx OTUB1 HSPC263, OTB1, OTU1 Add to Cart
26960Sgf11/pCDFsgf11 (Saccharomyces cerevisiae)Bacterial ExpressionStructural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. pCDF SGF11 Add to Cart
26961Sus1/PRSFSus1 (Saccharomyces cerevisiae)Bacterial ExpressionStructural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PRSF SUS1 YBR111W-A Add to Cart
26962Sgf73(1-106)/PET32aSgf73(1-106) (Saccharomyces cerevisiae)Bacterial ExpressionStructural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PET32a Add to Cart
26963Sus1/PET32aSus1 (Saccharomyces cerevisiae)Bacterial ExpressionStructural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PET32a SUS1 YBR111W-A Add to Cart
26964Sgf73_Sgf11/PCDFDuetsgf11 (Saccharomyces cerevisiae)Bacterial ExpressionStructural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. pCDFDuet SGF11 SGF73 YGL066W, SCA7 Add to Cart
26965Ubp8/PET32aUbp8 (Saccharomyces cerevisiae)Bacterial ExpressionStructural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PET32a UBP8 Add to Cart
34965Ube1/PET21dUbe1 (Homo sapiens)Bacterial ExpressionA spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2. PET21d UBA1 A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X Add to Cart
37194UbG75CUbiquitin G75C mutant (Homo sapiens)Bacterial ExpressionThe mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. PET3a Add to Cart
37195UbG76CUbiquitin G76C mutant (Homo sapiens)Bacterial ExpressionThe mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. PET3a
  • Tag / Fusion Protein
    • No tag
  • Add to Cart
    37196ceOtub1Otub1 ce (Caenorhabditis elegans)Bacterial ExpressionThe mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. pProEX HTa otub-1 C25D7.8 Add to Cart
    51131hUbc13ubc13 (Homo sapiens)Bacterial ExpressionThe mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. human ubc13, E2 for synthesis k63 linked ubiquitin chain PETSUMO UBE2N HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, UbcH13 Add to Cart
    60443UbcH5BUbcH5B (Homo sapiens)Bacterial ExpressionE2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. Express E2 UbcH5B in E. Coli PETSUMO Add to Cart
    61081UbcH5AUbcH5A (Homo sapiens)Bacterial ExpressionE2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. express a human E2 PETSUMO Add to Cart
    61088SENP2M497ASENP2M497A catalytic domain (Homo sapiens)Bacterial ExpressionE2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. cleave the SUMO off when use PETSUMO vector for express fusion protein PET28a SENP2 AXAM2, SMT3IP2 Add to Cart
    67241pp32ΔCTpp32 (Homo sapiens)Bacterial ExpressionThe crystal structure of the tumor suppressor protein pp32 (Anp32a): structural insights into Anp32 family of proteins. Protein Sci. 2007 Jul;16(7):1308-15. Epub 2007 Jun 13. pp32(1-149) cloned into pProEX HTb pProEX HTb ANP32A C15orf1, HPPCn, I1PP2A, LANP, MAPM, PHAP1, PHAPI, PP32 Add to Cart
    67242TbSIR2RP1TbSIR2RP1 (Other)Bacterial ExpressionSide chain specificity of ADP-ribosylation by a sirtuin. FEBS J. 2009 Dec;276(23):7159-76. doi: 10.1111/j.1742-4658.2009.07427.x. Epub 2009 Nov 6. Determine its activity and specificity pMalC2XHU Add to Cart
    75299His6TEV-UbG76Chuman Ubiquitin mutant G76 to C (Homo sapiens)Bacterial ExpressionStructural basis for histone H2B deubiquitination by the SAGA DUB module. Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681. Bacteria expression plasmid for Ubiquitin G76C with removalbe His6 tag by TEV protease pET3a Add to Cart
    75332H2BK120CH2BK120C (Xenopus laevis)Bacterial ExpressionStructural basis for histone H2B deubiquitination by the SAGA DUB module. Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681. Bacteria expression of H2BK120C pET3a hist1h2bj h2B, h2bfr Add to Cart