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Cynthia Wolberger Lab Plasmids


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Title Authors Publication
E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, Wolberger C Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18.
The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R, Zhang X, Wang T, Wolberger C Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911.
A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Berndsen CE, Wolberger C Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2.
Structural insights into the assembly and function of the SAGA deubiquitinating module. Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, Wolberger C Science. 2010 May 21. 328(5981):1025-9.
Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1. Wang T, Yin L, Cooper EM, Lai MY, Dickey S, Pickart CM, Fushman D, Wilkinson KD, Cohen RE, Wolberger C J Mol Biol. 2009 Mar 6. 386(4):1011-23.
Structural and functional studies of the Rap1 C-terminus reveal novel separation-of-function mutants. Feeser EA, Wolberger C J Mol Biol. 2008 Jul 11. 380(3):520-31.
Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C Nat Struct Mol Biol. 2006 Oct . 13(10):915-20.
Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C Nat Struct Mol Biol. 2006 Jul . 13(7):661-2.
Mechanism of Sirtuin Inhibition by Nicotinamide: Altering the NAD(+) Cosubstrate Specificity of a Sir2 Enzyme. Avalos JL, Bever KM, Wolberger C Mol Cell 2005 Mar 18;17(6):855-68.
Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. LaRonde-LeBlanc NA, Wolberger C Genes Dev 2003 Aug 15;17(16):2060-72.
Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera. Ke A, Wolberger C Protein Sci 2003 Feb;12(2):306-12.
Structure of a Sir2 enzyme bound to an acetylated p53 peptide. Avalos JL, Celic I, Muhammad S, Cosgrove MS, Boeke JD, Wolberger C Mol Cell 2002 Sep;10(3):523-35.
The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Batchelor AH, Piper DE, de la Brousse FC, McKnight SL, Wolberger C Science 1998 Feb 13;279(5353):1037-41.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
8380 pProEX-Af2Sir2 Sir2-Af2 full length Bacterial Expression Structure of a Sir2 enzyme bound to an acetylated p53 peptide. Mol Cell 2002 Sep;10(3):523-35. pProEXHT SIRT2 SIR2, SIR2L, SIR2L2 Sir2.2 SIRT1 SIR2L1 Add to Cart
8383 PET11a-Af2sir2 Sir2-Af2 full length Bacterial Expression Structure of a Sir2 enzyme bound to an acetylated p53 peptide. Mol Cell 2002 Sep;10(3):523-35. PET11a SIRT2 SIR2, SIR2L, SIR2L2 Sir2.2 SIRT1 SIR2L1 Add to Cart
8436 pMal-MATa1 MATa1(78aa-126aa) (Saccharomyces cerevisiae) Bacterial Expression Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera. Protein Sci 2003 Feb;12(2):306-12. modified pMal-c2x Add to Cart
8588 pA9HD HoxA9 190-271with hexapeptide (Homo sapiens) Bacterial Expression Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. Genes Dev 2003 Aug 15;17(16):2060-72. PET28a+ HOXA9 ABD-B, HOX1, HOX1.7, HOX1G Add to Cart
8589 pPBX1-318 Human pbx (1-318) (Homo sapiens) Bacterial Expression Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. Genes Dev 2003 Aug 15;17(16):2060-72. PET21b PBX1 Add to Cart
8813 PBB-79 GABPb1 (1-157) (Mus musculus) Bacterial Expression The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Science 1998 Feb 13;279(5353):1037-41. pG5' Gabpb1 E4TF1, GABPB, BABPB2, E4Tf1B, NRF2B1, NRF2B2, E4TF1-47, E4TF1-53, GABPB1-1, GABPB1-2 Add to Cart
8814 pBB-80 GABPa1 (318-430) (Mus musculus) Bacterial Expression The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Science 1998 Feb 13;279(5353):1037-41. pG5' Gabpa GABPalpha Add to Cart
25464 UBC13-PGEX4T Ubc13 (Saccharomyces cerevisiae) Bacterial Expression Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. pGEX4T UBC13 YDR092W MMS2 YGL087C Add to Cart
25465 MBP-His6-TEV-MMS2 MMS2 (Saccharomyces cerevisiae) Bacterial Expression Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. pMalC2XHV MMS2 YGL087C UBC13 YDR092W Add to Cart
25630 Nampt-PET28a Nampt (Mus musculus) Bacterial Expression Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Nat Struct Mol Biol. 2006 Jul . 13(7):661-2. PET28a Nampt 1110035O14Rik, AI314458, AI480535, NAmPRTase, Pbef, Pbef1, Visfatin Add to Cart
25815 TmSir2 Tm Sir2 Bacterial Expression Mechanism of Sirtuin Inhibition by Nicotinamide: Altering the NAD(+) Cosubstrate Specificity of a Sir2 Enzyme. Mol Cell 2005 Mar 18;17(6):855-68. PET11a Add to Cart
25817 pMal672H Rap1(672-827) (Saccharomyces cerevisiae) Bacterial Expression Structural and functional studies of the Rap1 C-terminus reveal novel separation-of-function mutants. J Mol Biol. 2008 Jul 11. 380(3):520-31. pDEST 14 Add to Cart
26959 hOtub1 Otub1 (Homo sapiens) Bacterial Expression Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1. J Mol Biol. 2009 Mar 6. 386(4):1011-23. pProEx OTUB1 HSPC263, OTB1, OTU1 Add to Cart
26960 Sgf11/pCDF sgf11 (Saccharomyces cerevisiae) Bacterial Expression Structural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. pCDF SGF11 Add to Cart
26961 Sus1/PRSF Sus1 (Saccharomyces cerevisiae) Bacterial Expression Structural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PRSF SUS1 YBR111W-A Add to Cart
26962 Sgf73(1-106)/PET32a Sgf73(1-106) (Saccharomyces cerevisiae) Bacterial Expression Structural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PET32a Add to Cart
26963 Sus1/PET32a Sus1 (Saccharomyces cerevisiae) Bacterial Expression Structural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PET32a SUS1 YBR111W-A Add to Cart
26964 Sgf73_Sgf11/PCDFDuet sgf11 (Saccharomyces cerevisiae) Bacterial Expression Structural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. pCDFDuet SGF11 SGF73 YGL066W, SCA7 Add to Cart
26965 Ubp8/PET32a Ubp8 (Saccharomyces cerevisiae) Bacterial Expression Structural insights into the assembly and function of the SAGA deubiquitinating module. Science. 2010 May 21. 328(5981):1025-9. PET32a UBP8 Add to Cart
34965 Ube1/PET21d Ube1 (Homo sapiens) Bacterial Expression A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2. PET21d UBA1 A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X Add to Cart
37194 UbG75C Ubiquitin G75C mutant (Homo sapiens) Bacterial Expression The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. PET3a Add to Cart
37195 UbG76C Ubiquitin G76C mutant (Homo sapiens) Bacterial Expression The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. PET3a
  • Tag / Fusion Protein
    • No tag
  • Add to Cart
    37196 ceOtub1 Otub1 ce (Caenorhabditis elegans) Bacterial Expression The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. pProEX HTa otub-1 C25D7.8 Add to Cart
    51131 hUbc13 ubc13 (Homo sapiens) Bacterial Expression The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. human ubc13, E2 for synthesis k63 linked ubiquitin chain PETSUMO UBE2N HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, UbcH13 Add to Cart
    60443 UbcH5B UbcH5B (Homo sapiens) Bacterial Expression E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. Express E2 UbcH5B in E. Coli PETSUMO Add to Cart
    61081 UbcH5A UbcH5A (Homo sapiens) Bacterial Expression E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. express a human E2 PETSUMO Add to Cart
    61088 SENP2M497A SENP2M497A catalytic domain (Homo sapiens) Bacterial Expression E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. cleave the SUMO off when use PETSUMO vector for express fusion protein PET28a Add to Cart