Cynthia Wolberger Lab Plasmids


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Title Authors Publication
The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R, Zhang X, Wang T, Wolberger C Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911.
A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Berndsen CE, Wolberger C Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2.
Structural insights into the assembly and function of the SAGA deubiquitinating module. Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, Wolberger C Science. 2010 May 21. 328(5981):1025-9.
Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1. Wang T, Yin L, Cooper EM, Lai MY, Dickey S, Pickart CM, Fushman D, Wilkinson KD, Cohen RE, Wolberger C J Mol Biol. 2009 Mar 6. 386(4):1011-23.
Structural and functional studies of the Rap1 C-terminus reveal novel separation-of-function mutants. Feeser EA, Wolberger C J Mol Biol. 2008 Jul 11. 380(3):520-31.
Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C Nat Struct Mol Biol. 2006 Oct . 13(10):915-20.
Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C Nat Struct Mol Biol. 2006 Jul . 13(7):661-2.
Mechanism of Sirtuin Inhibition by Nicotinamide: Altering the NAD(+) Cosubstrate Specificity of a Sir2 Enzyme. Avalos JL, Bever KM, Wolberger C Mol Cell 2005 Mar 18;17(6):855-68.
Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. LaRonde-LeBlanc NA, Wolberger C Genes Dev 2003 Aug 15;17(16):2060-72.
Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera. Ke A, Wolberger C Protein Sci 2003 Feb;12(2):306-12.
Structure of a Sir2 enzyme bound to an acetylated p53 peptide. Avalos JL, Celic I, Muhammad S, Cosgrove MS, Boeke JD, Wolberger C Mol Cell 2002 Sep;10(3):523-35.
The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Batchelor AH, Piper DE, de la Brousse FC, McKnight SL, Wolberger C Science 1998 Feb 13;279(5353):1037-41.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
8380 pProEX-Af2Sir2 Sir2-Af2 full length Bacterial Expression Avalos et al Mol Cell 2002 Sep;10(3):523-35. pProEXHT SIRT2 SIR2, SIR2L, SIR2L2 Sir2.2 SIRT1 RP11-57G10.3, SIR2L1
8383 PET11a-Af2sir2 Sir2-Af2 full length Bacterial Expression Avalos et al Mol Cell 2002 Sep;10(3):523-35. PET11a SIRT2 SIR2, SIR2L, SIR2L2 Sir2.2 SIRT1 RP11-57G10.3, SIR2L1
8436 pMal-MATa1 MATa1(78aa-126aa) (Saccharomyces cerevisiae) Bacterial Expression Ke et al Protein Sci 2003 Feb;12(2):306-12. modified pMal-c2x
8588 pA9HD HoxA9 190-271with hexapeptide (Homo sapiens) Bacterial Expression LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72. PET28a+ HOXA9 ABD-B, HOX1, HOX1.7, HOX1G
8589 pPBX1-318 Human pbx (1-318) (Homo sapiens) Bacterial Expression LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72. PET21b PBX1
8813 PBB-79 GABPb1 (1-157) (Mus musculus) Bacterial Expression Batchelor et al Science 1998 Feb 13;279(5353):1037-41. pG5' Gabpb1 E4TF1, GABPB, BABPB2, E4Tf1B, NRF2B1, NRF2B2, E4TF1-47, E4TF1-53, GABPB1-1, GABPB1-2
8814 pBB-80 GABPa1 (318-430) (Mus musculus) Bacterial Expression Batchelor et al Science 1998 Feb 13;279(5353):1037-41. pG5' Gabpa GABPalpha
25464 UBC13-PGEX4T Ubc13 (Saccharomyces cerevisiae) Bacterial Expression Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. pGEX4T UBC13 YDR092W MMS2 YGL087C
25465 MBP-His6-TEV-MMS2 MMS2 (Saccharomyces cerevisiae) Bacterial Expression Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. pMalC2XHV MMS2 YGL087C UBC13 YDR092W
25630 Nampt-PET28a Nampt (Mus musculus) Bacterial Expression Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):661-2. PET28a Nampt Pbef, Pbef1, AI314458, AI480535, Visfatin, NAmPRTase, 1110035O14Rik
25815 TmSir2 Tm Sir2 Bacterial Expression Avalos et al Mol Cell 2005 Mar 18;17(6):855-68. PET11a
25817 pMal672H Rap1(672-827) (Saccharomyces cerevisiae) Bacterial Expression Feeser et al J Mol Biol. 2008 Jul 11. 380(3):520-31. pDEST 14
26959 hOtub1 Otub1 (Homo sapiens) Bacterial Expression Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23. pProEx OTUB1 HSPC263, OTB1, OTU1
26960 Sgf11/pCDF sgf11 (Saccharomyces cerevisiae) Bacterial Expression Samara et al Science. 2010 May 21. 328(5981):1025-9. pCDF SGF11
26961 Sus1/PRSF Sus1 (Saccharomyces cerevisiae) Bacterial Expression Samara et al Science. 2010 May 21. 328(5981):1025-9. PRSF SUS1 YBR111W-A
26962 Sgf73(1-106)/PET32a Sgf73(1-106) (Saccharomyces cerevisiae) Bacterial Expression Samara et al Science. 2010 May 21. 328(5981):1025-9. PET32a
26963 Sus1/PET32a Sus1 (Saccharomyces cerevisiae) Bacterial Expression Samara et al Science. 2010 May 21. 328(5981):1025-9. PET32a SUS1 YBR111W-A
26964 Sgf73_Sgf11/PCDFDuet sgf11 (Saccharomyces cerevisiae) Bacterial Expression Samara et al Science. 2010 May 21. 328(5981):1025-9. pCDFDuet SGF11 SGF73 YGL066W, SCA7
26965 Ubp8/PET32a Ubp8 (Saccharomyces cerevisiae) Bacterial Expression Samara et al Science. 2010 May 21. 328(5981):1025-9. PET32a UBP8
34965 Ube1/PET21d Ube1 (Homo sapiens) Bacterial Expression Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2. PET21d UBA1 CTD-2522E6.1, A1S9, A1S9T, A1ST, AMCX1, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X
37194 UbG75C Ubiquitin G75C mutant (Homo sapiens) Bacterial Expression Wiener et al Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. PET3a
37195 UbG76C Ubiquitin G76C mutant (Homo sapiens) Bacterial Expression Wiener et al Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. PET3a
  • Tag / Fusion Protein
    • No tag
  • 37196 ceOtub1 Otub1 ce (Caenorhabditis elegans) Bacterial Expression Wiener et al Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. pProEX HTa otub-1 C25D7.8