This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Erik Sontheimer Lab Plasmids


The Erik Sontheimer Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

Learn more about research in the Erik Sontheimer Lab.

Addgene Alerts

Receive email alerts when new plasmids from this lab become available.

Log in to subscribe to Addgene Alerts.

Title Authors Publication
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA Proc Natl Acad Sci U S A. 2013 Aug 12.
DNase H Activity of Neisseria meningitidis Cas9. Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020.
Naturally Occurring Off-Switches for CRISPR-Cas9. Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
47867pSimpleII-NLS-NmCas9-HA-NLS(s)NmCas9 (Other)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid encode NmCas9 with two NLS and a HA tag pSimpleII Add to Cart
47868pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)NmCas9 (Other), U6pr-tracrRNA (Other)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter. pSimpleII Add to Cart
47869pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)NmCas9 (Other), U6pr-tracrRNA (Other), tdTomato crRNA (Synthetic)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. All-in-one plasmid targeting tdTomato pSimpleII Add to Cart
47870pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)NmCas9 (Other), U6pr-tracrRNA (Other), OCT4 crRNA (Synthetic)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon. pSimpleII Add to Cart
47871pSTBlue-U6-sgRNA(tdTomato)sgRNA-tdTomato (Synthetic)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid contains expression cassette for sgRNA targeting tdTomato. pstBlue-1 Add to Cart
47872pSimpleII-NmCas9-FLAGNmCas9 (Other)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. FLAG tagged NmCas9 with no NLS. pSimpleII Add to Cart
71474pMCSG7-Wt-NmeCas9pMCSG7-WT-NmeCas9 (Other)Bacterial ExpressionDNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site pMCSG7 Add to Cart
71475pMCSG7-dNmeCas9pMCSG7-Inactive-NmeCas9 (Other)Bacterial ExpressionDNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site pMCSG7 Add to Cart
71476pMCSG7-D16A-NmeCas9pMCSG7-D16A-NmeCas9 (Other)Bacterial ExpressionDNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site pMCSG7 Add to Cart
71477pMCSG7-H588A-NmeCas9pMCSG7-H588A-NmeCas9 (Other)Bacterial ExpressionDNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site pMCSG7 Add to Cart
85677pEJS427-pCSDest2-AcrE2Type I-E anti-CRISPR (Other)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Mammalian expression of anti-CRISPR protein from Type I-E pCSDest2 Add to Cart
85678pEJS430-pCSDest2-AcrIIC1BoeType II-C anti-CRISPR AcrIIC1Boe (Other)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis pCSDest2 Add to Cart
85679pEJS433-pCSDest2-AcrIIC1NmeType II-C anti-CRISPR AcrIIC1Nme (Other)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis pCSDest2 Add to Cart
85712pEJS436-pCSDest2-AcrIIC2NmeType II-C anti-CRISPR AcrIIC2Nme (Other)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis pCSDest2 Add to Cart
85713pEJS443-pCSDest2-AcrIIC3NmeType II-C anti-CRISPR AcrIIC3Nme (Other)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis pCSDest2 Add to Cart
85714pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomeretelomere sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Telomere-targeting Nme sgRNA under U6 promoter pLKO.1 Add to Cart
85715pEJS469-pLK.O1-SpySgRNA/DTS13-Telomeretelomere sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Telomere-targeting Spy sgRNA under U6 promoter pLKO.1 Add to Cart
85716pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-oneNme dCas9 (Synthetic), telomere sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA pHAGE-DEST Add to Cart
85717pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-oneSp dCas9 (Synthetic), telomere sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA pHAGE-DEST Add to Cart
85748pEJS507-pCDest2-noAcr-mTagBFP2-IRESmTagBFP2 (Synthetic)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Expresses mTagBFP2 only; used as a control pCSDest2 Add to Cart
85749pEJS481-pCDest2-AcrE2-mTagBFP2-IRESType I-E anti-CRISPR (Other), mTagBFP2 (Synthetic)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Expresses anti-CRISPR protein from Type I-E and mTagBFP2 pCSDest2 Add to Cart
85750pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRESType II-C anti-CRISPR AcrIIC3Nme (Other), mTagBFP2 (Synthetic)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2 pCSDest2 Add to Cart
87448pCSDest2-NmeCas9-NLS-3XHA-NLSNme Cas9 (Other)Mammalian Expression, CRISPRNaturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Mammalian expression of wild type Nme Cas9 pCSDest2 Add to Cart