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Frank Schnorrer Lab Plasmids


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Title Authors Publication
A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. Zhang X, Koolhaas WH, Schnorrer F G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
60944pJET1.2-STOP-dsReddsRED (Other)UnspecifiedA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR. pJET1.2 Add to Cart
60945pBS-GGAC-TTCTCRISPRA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. Drosophila, backbone for donor plasmid assembly. CRISPR knock-in. pBluescript II Add to Cart
60948p3xP3-EGFP.vas-int.NLSvas-phiC31 integrase.NLS (Other)RMCEA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR. pBluescript II Add to Cart
60949pBS-GGAC-ATGCCRISPRA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. Drosophila, backbone for donor plasmid assembly. CRISPR knock-in. pBluescript II Add to Cart
60950pBS-CGGA-GTGCCRISPRA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. Drosophila, backbone for donor plasmid assembly. CRISPR knock-in. pBluescript II Add to Cart
61255pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V52xTY1-V5 (Synthetic)CRISPRA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette pBS-KS-attB1-2-PT-SA-SD Add to Cart
61256pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V52xTY1-V5 (Synthetic)CRISPRA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette pBS-KS-attB1-2-PT-SA-SD Add to Cart
61257pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V52xTY1-V5 (Synthetic)CRISPRA Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette pBS-KS-attB1-2-PT-SA-SD Add to Cart