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Gary Yellen Lab Plasmids


The Gary Yellen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A genetically encoded fluorescent reporter of ATP:ADP ratio. Berg J, Hung YP, Yellen G Nat Methods. 2009 Feb . 6(2):161-6.
Imaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor. Tantama M, Hung YP, Yellen G J Am Chem Soc. 2011 Jul 6. 133(26):10034-7.
Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G Cell Metab. 2011 Oct 5;14(4):545-54.
Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Tantama M, Martinez-Francois JR, Mongeon R, Yellen G Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550.
Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging. Mongeon R, Venkatachalam V, Yellen G Antioxid Redox Signal. 2016 Mar 18.
pXP420 repaired Amp promoter (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
20336pRsetB-his7-PercevalPerceval (fluorescent ATP:ADP sensor) (Synthetic)Bacterial ExpressionA genetically encoded fluorescent reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2):161-6. pRset B Add to Cart
21737GW1CMV-PercevalPerceval (fluorescent ATP:ADP sensor) (Synthetic)Mammalian ExpressionA genetically encoded fluorescent reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2):161-6. can be used to monitor the ATP:ADP ratio during live-cell imaging pGW1-CMV Add to Cart
31472pRsetB-pHRedpHRed (Synthetic)Bacterial ExpressionImaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor. J Am Chem Soc. 2011 Jul 6. 133(26):10034-7. pRsetB Add to Cart
31473GW1-pHRedpHRed (Synthetic)Mammalian ExpressionImaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor. J Am Chem Soc. 2011 Jul 6. 133(26):10034-7. genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima GW1 Add to Cart
31474GW1-Mito-pHRedCox8-tagged pHRed (Synthetic)Mammalian ExpressionImaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor. J Am Chem Soc. 2011 Jul 6. 133(26):10034-7. GW1 Add to Cart
32380GW1-Peredox-mCherryPeredox-mCherryMammalian ExpressionImaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. GW1 Add to Cart
32381GW1-Peredox-mCherry-NLSPeredox-mCherry-NLSMammalian ExpressionImaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. GW1 Add to Cart
32382pRsetB-His7tag-Peredox-mCherryHis7-Peredox-mCherryBacterial ExpressionImaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. pRSetB Add to Cart
32383pcDNA3.1-Peredox-mCherryPeredox-mCherryMammalian ExpressionImaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. pcDNA3.1 Add to Cart
32384pcDNA3.1-Peredox-mCherry-NLSPeredox-mCherry-NLSMammalian ExpressionImaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. pcDNA3.1 Add to Cart
32385pMSCV-Peredox-mCherry-NLSPeredox-mCherry-NLSMammalian Expression, RetroviralImaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. pMSCV Add to Cart
32386GW1-Peredox-mCitrinePeredox-mCitrineMammalian ExpressionImaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. GW1 Add to Cart
49081pRsetB-PercevalHRPercevalHR (Synthetic)Bacterial ExpressionImaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio. pRsetB Add to Cart
49082GW1-PercevalHRPercevalHR (Synthetic)Mammalian ExpressionImaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio. GW1 Add to Cart
49083FUGW-PercevalHRPercevalHR (Synthetic)Mammalian Expression, LentiviralImaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio. FUGW Add to Cart
73806pRSetB-PeredoxPeredox (Synthetic)Bacterial ExpressionCytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging. Antioxid Redox Signal. 2016 Mar 18. Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag pRSetB Add to Cart
73807pAAV-CAG-PeredoxPeredox (Synthetic)Mammalian Expression, AAVCytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging. Antioxid Redox Signal. 2016 Mar 18. Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV) AAV-Flex Add to Cart
86920pXP420 v2 (repaired Amp promoter)Yeast Expression, Cre/Lox pXP420 repaired Amp promoter (unpublished) Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker. pUC18 Add to Cart