Guy Salvesen Lab Plasmids


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Title Authors Publication
Mechanism and specificity of the human paracaspase MALT1. Hachmann J, Snipas SJ, van Raam BJ, Cancino EM, Houlihan EJ, Poreba M, Kasperkiewicz P, Drag M, Salvesen GS Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035.
The dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. Bekes M, Prudden J, Srikumar T, Raught B, Boddy MN, Salvesen GS J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19.
Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS J Biol Chem. 2007 Sep 7. 282(36):26217-24.
The apoptosome activates caspase-9 by dimerization. Pop C, Timmer J, Sperandio S, Salvesen GS Mol Cell. 2006 Apr 21. 22(2):269-75.
Activation of caspases-8 and -10 by FLIP(L). Boatright KM, Deis C, Denault JB, Sutherlin DP, Salvesen GS Biochem J. 2004 Sep 1. 382(Pt 2):651-7.
Mechanism-based inactivation of caspases by the apoptotic suppressor p35. Riedl SJ, Renatus M, Snipas SJ, Salvesen GS Biochemistry. 2001 Nov 6. 40(44):13274-80.
Caspase-9 can be activated without proteolytic processing. Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS J Biol Chem. 1999 Mar 26. 274(13):8359-62.
Biochemical characteristics of caspases-3, -6, -7, and -8. Stennicke HR, Salvesen GS J Biol Chem. 1997 Oct 10. 272(41):25719-23.
Target protease specificity of the viral serpin CrmA. Analysis of five caspases. Zhou Q, Snipas S, Orth K, Muzio M, Dixit VM, Salvesen GS J Biol Chem. 1997 Mar 21. 272(12):7797-800.
FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. Muzio M, Salvesen GS, Dixit VM J Biol Chem. 1997 Jan 31. 272(5):2952-6.
Salvesen Xiap plasmids (unpublished)
Salvesen Casp1, Casp2 plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
11808 pET21b-P35 P35 Bacterial Expression Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-80. pET-21 b
11809 pET21b-Casp1-His Caspase-1 (Homo sapiens) Bacterial Expression Salvesen Casp1, Casp2 plasmids (unpublished) pET-21 b CASP1 ICE, IL1BC, P45
11810 pET23b-Casp2-Delta Card Caspase-2 Delta Card (Homo sapiens) Bacterial Expression Salvesen Casp1, Casp2 plasmids (unpublished) pET-23 b CASP2 CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57
11811 pcDNA3-Casp2-Flag Caspase-2 (Homo sapiens) Mammalian Expression Salvesen Casp1, Casp2 plasmids (unpublished) pcDNA3 CASP2 CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57
11812 pcDNA3-Casp2 C303A-Flag Caspase-2 catalytic mutant (Homo sapiens) Mammalian Expression Salvesen Casp1, Casp2 plasmids (unpublished) pcDNA3 CASP2 CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57
11813 pcDNA3-Casp3-myc Caspase 3 (Homo sapiens) Mammalian Expression Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP3 CPP32, CPP32B, SCA-1
11814 pcDNA3-Casp3 C163A-myc Caspase 3 catalytic mutant (Homo sapiens) Mammalian Expression Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP3 CPP32, CPP32B, SCA-1
11815 pcDNA3-Casp7-Flag Caspase 7 (Homo sapiens) Mammalian Expression Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP7 RP11-211N11.6, CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3
11816 pcDNA3-Casp7 C186A-Flag Caspase 7 catalytic mutant (Homo sapiens) Mammalian Expression Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP7 RP11-211N11.6, CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3
11817 pcDNA3-Casp8 Caspase 8 (Homo sapiens) Mammalian Expression Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
11818 pcDNA3-Casp8 C360A Caspase 8 catalytic mutant (Homo sapiens) Mammalian Expression Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
11819 pcDNA3-Casp9 C287A Caspase 9 catalytically incompetent mutant (Homo sapiens) Mammalian Expression Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-62. pcDNA3 CASP9 RP11-265F14.3, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
11821 pET23b-Casp3-His Caspase 3 (Homo sapiens) Bacterial Expression Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP3 CPP32, CPP32B, SCA-1
11822 pET23b-Casp3 C163A-His Caspase 3 catalytic mutant (Homo sapiens) Bacterial Expression Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP3 CPP32, CPP32B, SCA-1
11823 pET23b-Casp6-His Caspase 6 (Homo sapiens) Bacterial Expression Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP6 MCH2
11825 pET23b-Casp7-His Caspase 7 (Homo sapiens) Bacterial Expression Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP7 RP11-211N11.6, CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3
11826 pET23b-Casp7 C186A-His Caspase 7 catalytic mutant (Homo sapiens) Bacterial Expression Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP7 RP11-211N11.6, CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3
11827 pET15b-Casp8 delta DED Caspase 8 minus Death Effecter Domain (Homo sapiens) Bacterial Expression Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-15 b CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
11828 pET15b-Casp8 delta DED C360A Caspase 8 minus Death Effecter Domain catalytic mutant (Homo sapiens) Bacterial Expression Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-15 b CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
11829 pET23b-Casp9-His Caspase 9 (Homo sapiens) Bacterial Expression Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-62. pET-23 b CASP9 RP11-265F14.3, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
11830 pET23b-Casp9 C287A-His Caspase 9 catalytic mutant (Homo sapiens) Bacterial Expression Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-62. pET-23 b CASP9 RP11-265F14.3, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
11831 pFLAG-CrmA CrmA Bacterial Expression Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6. pFLAG CrmA CPXV207
11832 pcDNA3-CrmA CrmA Mammalian Expression Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6. pcDNA3 CrmA CPXV207
11833 pcDNA3-Xiap-Myc XIAP (Homo sapiens) Mammalian Expression Salvesen Xiap plasmids (unpublished) pcDNA3.1 XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
11834 pcDNA3-Xiap-Myc K322/328R XIAP K322/328R (Homo sapiens) Mammalian Expression Salvesen Xiap plasmids (unpublished) pcDNA3.1 XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
13790 Delta DED FLIP-L DELTA DED FLIP LONG (Homo sapiens) Bacterial Expression Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7. pET15b
13791 Delta DED FLIP-L D/A DELTA DED FLIP LONG (Homo sapiens) Bacterial Expression Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7. pET15b
16356 pET28a-SENP1 (catalytic domain) human SENP1 catalytic domain (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP1 SuPr-2
16357 pET28a-SENP2 (catalytic domain) human SENP2 catalytic domain (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP2 AXAM2, SMT3IP2
16358 pET28a-SENP5 (catalytic domain) human SENP5 catalytic domain (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP5 FKSG45
16359 pET28a-SENP6 (catalytic domain) human SENP6 catalytic domain (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP6 RP1-134M13.1, SSP1, SUSP1
16360 pET28a-SENP7 (catalytic domain) human SENP7 catalytic domain (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP7
16361 pET28a-SENP8 (full length)) human SENP8 full length (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP8 FKSG8, DEN1, NEDP1, PRSC2
25101 pET28a-pro-sumo1 pro-SUMO1 (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SUMO1 OK/SW-cl.43, DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
25102 pET28a-pro-sumo2 pro-SUMO2 (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SUMO2 HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A
25103 pET28a-pro-sumo3 pro-SUMO3 (Homo sapiens) Bacterial Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SUMO3 SMT3A, SMT3H1, SUMO-3, Smt3B
25104 pcDNA3flag-RanGAPdeltaN RanGAP1 deleted N-term domain (Homo sapiens) Mammalian Expression Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24. pcDNA3
26642 pET21b Apaf1 1-591 Apoptotic protease activating factor 1 (Homo sapiens) Bacterial Expression Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75. pET21b APAF1 APAF-1, CED4
36448 pcDNA3 HA-SUMO2-Q90P SUMO2 (Homo sapiens) Mammalian Expression Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA-Sumo2Q90P mutant in mammalian cells pcDNA3.1 SUMO2 HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A
48963 pcDNA3 MYC-Sumo3Q89P SUMO3 (Homo sapiens) Mammalian Expression Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses MYC-Sumo3Q90P mutant in mammalian cells pcDNA3.1 SUMO3 SMT3A, SMT3H1, SUMO-3, Smt3B
48964 pcDNA3 MYC-Sumo3 WT SUMO3 (Homo sapiens) Mammalian Expression Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses MYC-Sumo3 Wild Type in mammalian cells pcDNA3.1 SUMO3 SMT3A, SMT3H1, SUMO-3, Smt3B
48965 pcDNA3 HA-Sumo1 Q94P SUMO1 (Homo sapiens) Mammalian Expression Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA SUMO1 Q94P mutant in mammalian cells pcDNA3.1 SUMO1 OK/SW-cl.43, DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
48966 pcDNA3 HA-Sumo1 WT SUMO1 (Homo sapiens) Mammalian Expression Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA-Sumo1 WT in mammalian cells pcDNA3.1 SUMO1 OK/SW-cl.43, DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
48967 pcDNA3 HA-Sumo2 WT SUMO2 (Homo sapiens) Mammalian Expression Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA SUMO2 Wild Type in mammalian cells pcDNA3.1 SUMO2 HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A
48968 FL MALT1 WT in pET29b-Strep MALT1 isoform A (Homo sapiens) Bacterial Expression Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses Full length wild type MALT1 in e.coli pET29b MALT1 IMD12, MLT, MLT1
48969 FL MALT1 Catalytic Mutant in pET29b-Strep MALT1 isoform A (Homo sapiens) Bacterial Expression Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses Full length catalytic mutant MALT1 in e.coli pET29b MALT1 IMD12, MLT, MLT1
48970 pET21 Catalytic Domain of MALT1 MALT1 (Homo sapiens) Bacterial Expression Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses catalytic domain of MALT1 in e.coli AA 329-566 pET21b MALT1 IMD12, MLT, MLT1
48971 pET21 Catalytic Domain of MALT1 Catalytic Mutant MALT1 (Homo sapiens) Bacterial Expression Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566 pET21b MALT1 IMD12, MLT, MLT1