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Guy Salvesen Lab Plasmids


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Title Authors Publication
Mechanism and specificity of the human paracaspase MALT1. Hachmann J, Snipas SJ, van Raam BJ, Cancino EM, Houlihan EJ, Poreba M, Kasperkiewicz P, Drag M, Salvesen GS Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035.
The dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. Bekes M, Prudden J, Srikumar T, Raught B, Boddy MN, Salvesen GS J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19.
Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS J Biol Chem. 2007 Sep 7. 282(36):26217-24.
The apoptosome activates caspase-9 by dimerization. Pop C, Timmer J, Sperandio S, Salvesen GS Mol Cell. 2006 Apr 21. 22(2):269-75.
Activation of caspases-8 and -10 by FLIP(L). Boatright KM, Deis C, Denault JB, Sutherlin DP, Salvesen GS Biochem J. 2004 Sep 1. 382(Pt 2):651-7.
Mechanism-based inactivation of caspases by the apoptotic suppressor p35. Riedl SJ, Renatus M, Snipas SJ, Salvesen GS Biochemistry. 2001 Nov 6. 40(44):13274-80.
Caspase-9 can be activated without proteolytic processing. Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS J Biol Chem. 1999 Mar 26. 274(13):8359-62.
Biochemical characteristics of caspases-3, -6, -7, and -8. Stennicke HR, Salvesen GS J Biol Chem. 1997 Oct 10. 272(41):25719-23.
Target protease specificity of the viral serpin CrmA. Analysis of five caspases. Zhou Q, Snipas S, Orth K, Muzio M, Dixit VM, Salvesen GS J Biol Chem. 1997 Mar 21. 272(12):7797-800.
FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. Muzio M, Salvesen GS, Dixit VM J Biol Chem. 1997 Jan 31. 272(5):2952-6.
Salvesen Xiap plasmids (unpublished)
Salvesen Casp1, Casp2 plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
11808pET21b-P35P35Bacterial ExpressionMechanism-based inactivation of caspases by the apoptotic suppressor p35. Biochemistry. 2001 Nov 6. 40(44):13274-80. pET-21 b Add to Cart
11809pET21b-Casp1-HisCaspase-1 (Homo sapiens)Bacterial Expression Salvesen Casp1, Casp2 plasmids (unpublished) pET-21 b CASP1 ICE, IL1BC, P45 Add to Cart
11810pET23b-Casp2-Delta CardCaspase-2 Delta Card (Homo sapiens)Bacterial Expression Salvesen Casp1, Casp2 plasmids (unpublished) pET-23 b CASP2 CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57 Add to Cart
11811pcDNA3-Casp2-FlagCaspase-2 (Homo sapiens)Mammalian Expression Salvesen Casp1, Casp2 plasmids (unpublished) pcDNA3 CASP2 CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57 Add to Cart
11812pcDNA3-Casp2 C303A-FlagCaspase-2 catalytic mutant (Homo sapiens)Mammalian Expression Salvesen Casp1, Casp2 plasmids (unpublished) pcDNA3 CASP2 CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57 Add to Cart
11813pcDNA3-Casp3-mycCaspase 3 (Homo sapiens)Mammalian ExpressionBiochemical characteristics of caspases-3, -6, -7, and -8. J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP3 CPP32, CPP32B, SCA-1 Add to Cart
11814pcDNA3-Casp3 C163A-mycCaspase 3 catalytic mutant (Homo sapiens)Mammalian ExpressionBiochemical characteristics of caspases-3, -6, -7, and -8. J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP3 CPP32, CPP32B, SCA-1 Add to Cart
11815pcDNA3-Casp7-FlagCaspase 7 (Homo sapiens)Mammalian ExpressionBiochemical characteristics of caspases-3, -6, -7, and -8. J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 Add to Cart
11816pcDNA3-Casp7 C186A-FlagCaspase 7 catalytic mutant (Homo sapiens)Mammalian ExpressionBiochemical characteristics of caspases-3, -6, -7, and -8. J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 Add to Cart
11817pcDNA3-Casp8Caspase 8 (Homo sapiens)Mammalian ExpressionBiochemical characteristics of caspases-3, -6, -7, and -8. J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 Add to Cart
11818pcDNA3-Casp8 C360ACaspase 8 catalytic mutant (Homo sapiens)Mammalian ExpressionBiochemical characteristics of caspases-3, -6, -7, and -8. J Biol Chem. 1997 Oct 10. 272(41):25719-23. pcDNA3 CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 Add to Cart
11819pcDNA3-Casp9 C287ACaspase 9 catalytically incompetent mutant (Homo sapiens)Mammalian ExpressionCaspase-9 can be activated without proteolytic processing. J Biol Chem. 1999 Mar 26. 274(13):8359-62. pcDNA3 CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 Add to Cart
11821pET23b-Casp3-HisCaspase 3 (Homo sapiens)Bacterial ExpressionTarget protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP3 CPP32, CPP32B, SCA-1 Add to Cart
11822pET23b-Casp3 C163A-HisCaspase 3 catalytic mutant (Homo sapiens)Bacterial ExpressionTarget protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP3 CPP32, CPP32B, SCA-1 Add to Cart
11823pET23b-Casp6-HisCaspase 6 (Homo sapiens)Bacterial ExpressionTarget protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP6 MCH2 Add to Cart
11825pET23b-Casp7-HisCaspase 7 (Homo sapiens)Bacterial ExpressionTarget protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 Add to Cart
11826pET23b-Casp7 C186A-HisCaspase 7 catalytic mutant (Homo sapiens)Bacterial ExpressionTarget protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-23 b CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 Add to Cart
11827pET15b-Casp8 delta DEDCaspase 8 minus Death Effecter Domain (Homo sapiens)Bacterial ExpressionTarget protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-15 b CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 Add to Cart
11828pET15b-Casp8 delta DED C360ACaspase 8 minus Death Effecter Domain catalytic mutant (Homo sapiens)Bacterial ExpressionTarget protease specificity of the viral serpin CrmA. Analysis of five caspases. J Biol Chem. 1997 Mar 21. 272(12):7797-800. pET-15 b CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 Add to Cart
11829pET23b-Casp9-HisCaspase 9 (Homo sapiens)Bacterial ExpressionCaspase-9 can be activated without proteolytic processing. J Biol Chem. 1999 Mar 26. 274(13):8359-62. pET-23 b CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 Add to Cart
11830pET23b-Casp9 C287A-HisCaspase 9 catalytic mutant (Homo sapiens)Bacterial ExpressionCaspase-9 can be activated without proteolytic processing. J Biol Chem. 1999 Mar 26. 274(13):8359-62. pET-23 b CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 Add to Cart
11831pFLAG-CrmACrmABacterial ExpressionFLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. J Biol Chem. 1997 Jan 31. 272(5):2952-6. pFLAG CrmA CPXV207 Add to Cart
11832pcDNA3-CrmACrmAMammalian ExpressionFLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. J Biol Chem. 1997 Jan 31. 272(5):2952-6. pcDNA3 CrmA CPXV207 Add to Cart
11833pcDNA3-Xiap-MycXIAP (Homo sapiens)Mammalian Expression Salvesen Xiap plasmids (unpublished) pcDNA3.1 XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
11834pcDNA3-Xiap-Myc K322/328RXIAP K322/328R (Homo sapiens)Mammalian Expression Salvesen Xiap plasmids (unpublished) pcDNA3.1 XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
13790Delta DED FLIP-LDELTA DED FLIP LONG (Homo sapiens)Bacterial ExpressionActivation of caspases-8 and -10 by FLIP(L). Biochem J. 2004 Sep 1. 382(Pt 2):651-7. pET15b Add to Cart
13791Delta DED FLIP-L D/ADELTA DED FLIP LONG (Homo sapiens)Bacterial ExpressionActivation of caspases-8 and -10 by FLIP(L). Biochem J. 2004 Sep 1. 382(Pt 2):651-7. pET15b Add to Cart
16356pET28a-SENP1 (catalytic domain)human SENP1 catalytic domain (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP1 SuPr-2 Add to Cart
16357pET28a-SENP2 (catalytic domain)human SENP2 catalytic domain (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP2 AXAM2, SMT3IP2 Add to Cart
16358pET28a-SENP5 (catalytic domain)human SENP5 catalytic domain (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP5 Add to Cart
16359pET28a-SENP6 (catalytic domain)human SENP6 catalytic domain (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP6 SSP1, SUSP1 Add to Cart
16360pET28a-SENP7 (catalytic domain)human SENP7 catalytic domain (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP7 Add to Cart
16361pET28a-SENP8 (full length))human SENP8 full length (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SENP8 DEN1, NEDP1, PRSC2 Add to Cart
25101pET28a-pro-sumo1pro-SUMO1 (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 Add to Cart
25102pET28a-pro-sumo2pro-SUMO2 (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SUMO2 HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A Add to Cart
25103pET28a-pro-sumo3pro-SUMO3 (Homo sapiens)Bacterial ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pET28a SUMO3 SMT3A, SMT3H1, SUMO-3, Smt3B Add to Cart
25104pcDNA3flag-RanGAPdeltaNRanGAP1 deleted N-term domain (Homo sapiens)Mammalian ExpressionSmall ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. J Biol Chem. 2007 Sep 7. 282(36):26217-24. pcDNA3 Add to Cart
26642pET21b Apaf1 1-591Apoptotic protease activating factor 1 (Homo sapiens)Bacterial ExpressionThe apoptosome activates caspase-9 by dimerization. Mol Cell. 2006 Apr 21. 22(2):269-75. pET21b APAF1 APAF-1, CED4 Add to Cart
36448pcDNA3 HA-SUMO2-Q90PSUMO2 (Homo sapiens)Mammalian ExpressionThe dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA-Sumo2Q90P mutant in mammalian cells pcDNA3.1 SUMO2 HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A Add to Cart
48963pcDNA3 MYC-Sumo3Q89PSUMO3 (Homo sapiens)Mammalian ExpressionThe dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses MYC-Sumo3Q90P mutant in mammalian cells pcDNA3.1 SUMO3 SMT3A, SMT3H1, SUMO-3, Smt3B Add to Cart
48964pcDNA3 MYC-Sumo3 WTSUMO3 (Homo sapiens)Mammalian ExpressionThe dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses MYC-Sumo3 Wild Type in mammalian cells pcDNA3.1 SUMO3 SMT3A, SMT3H1, SUMO-3, Smt3B Add to Cart
48965pcDNA3 HA-Sumo1 Q94PSUMO1 (Homo sapiens)Mammalian ExpressionThe dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA SUMO1 Q94P mutant in mammalian cells pcDNA3.1 SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 Add to Cart
48966pcDNA3 HA-Sumo1 WTSUMO1 (Homo sapiens)Mammalian ExpressionThe dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA-Sumo1 WT in mammalian cells pcDNA3.1 SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 Add to Cart
48967pcDNA3 HA-Sumo2 WTSUMO2 (Homo sapiens)Mammalian ExpressionThe dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. Expresses HA SUMO2 Wild Type in mammalian cells pcDNA3.1 SUMO2 HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A Add to Cart
48968FL MALT1 WT in pET29b-StrepMALT1 isoform A (Homo sapiens)Bacterial ExpressionMechanism and specificity of the human paracaspase MALT1. Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses Full length wild type MALT1 in e.coli pET29b MALT1 IMD12, MLT, MLT1 Add to Cart
48969FL MALT1 Catalytic Mutant in pET29b-StrepMALT1 isoform A (Homo sapiens)Bacterial ExpressionMechanism and specificity of the human paracaspase MALT1. Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses Full length catalytic mutant MALT1 in e.coli pET29b MALT1 IMD12, MLT, MLT1 Add to Cart
48970pET21 Catalytic Domain of MALT1MALT1 (Homo sapiens)Bacterial ExpressionMechanism and specificity of the human paracaspase MALT1. Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses catalytic domain of MALT1 in e.coli AA 329-566 pET21b MALT1 IMD12, MLT, MLT1 Add to Cart
48971pET21 Catalytic Domain of MALT1 Catalytic MutantMALT1 (Homo sapiens)Bacterial ExpressionMechanism and specificity of the human paracaspase MALT1. Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566 pET21b MALT1 IMD12, MLT, MLT1 Add to Cart