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Hideo Iwai Lab Plasmids


The Hideo Iwai Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Zuger S, Iwai H Nat Biotechnol. 2005 Jun . 23(6):736-40.
Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. Iwai H, Zuger S, Jin J, Tam PH FEBS Lett. 2006 Mar 20. 580(7):1853-8.
Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Muona M, Aranko AS, Iwai H Chembiochem. 2008 Dec 15. 9(18):2958-61.
Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Muona M, Aranko AS, Raulinaitis V, Iwaï H Nat Protoc. 2010 . 5(3):574-87.
Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. Ellila S, Jurvansuu JM, Iwai H FEBS Lett. 2011 Oct 10.
NMR resonance assignment of DnaE intein from Nostoc punctiforme. Heinamaki K, Oeemig JS, Paakkonen K, Djupsjobacka J, Iwai H Biomol NMR Assign. 2009 Jun;3(1):41-3. doi: 10.1007/s12104-008-9137-1. Epub 2009 Jan 13.
Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H FEBS Lett. 2009 May 6;583(9):1451-6. doi: 10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1.
Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
Structure-based engineering and comparison of novel split inteins for protein ligation. Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
Tandem SUMO fusion vectors for improving soluble protein expression and purification. Guerrero F, Ciragan A, Iwai H Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
11962pJJDuet30Ssp DnaE-NBacterial ExpressionIntein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Nat Biotechnol. 2005 Jun . 23(6):736-40. pRSFDuet-1 Add to Cart
11963pSFBAD09SspDnaECBacterial ExpressionIntein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Nat Biotechnol. 2005 Jun . 23(6):736-40. pBAD Add to Cart
11964pSZBAD1PGSspDnaECBacterial ExpressionIntein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Nat Biotechnol. 2005 Jun . 23(6):736-40. pBAD Add to Cart
12172pSKDuet01NpuDnaEBacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pRSFDuet Add to Cart
15335pSKBAD2DnaE C-inteinBacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15336pSZBAD43DnaE C-intein(CYN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15337pSZBAD44DnaE C-intein(CTN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15338pSZBAD45DnaE C-intein(CRN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15339pSZBAD47DnaE C-intein(CWN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15340pSZBAD48DnaE C-intein(CVN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15341pSZBAD49DnaE C-intein(CPN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15342pSZBAD50DnaE C-intein(CDN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15343pSZBAD51DnaE C-intein(CNN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15344pJJBAD78DnaE C-intein(CMN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15345pJJBAD79DnaE C-intein(CKN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15346pJJBAD80DnaE C-intein(CAN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15347pJJBAD81DnaE C-intein(CQN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15348pJJBAD82DnaE C-intein(CEN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15349pJJBAD83DnaE C-intein(CHN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15350pJJBAD84DnaE C-intein(CIN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15351pJJBAD85DnaE C-intein(CLN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15352pJJBAD86DnaE C-intein(CCN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15353pJJBAD87DnaE C-intein(CSN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
15354pJJBAD88DnaE C-intein(CGN)Bacterial ExpressionHighly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett. 2006 Mar 20. 580(7):1853-8. pBAD Add to Cart
20176pMMBAD16src homology domain 3 (Homo sapiens)Bacterial ExpressionSegmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Chembiochem. 2008 Dec 15. 9(18):2958-61. pBAD CRK CRKII, p38 Add to Cart
20177pMMRSF1-16src homology domain 3 (Homo sapiens)Bacterial ExpressionSegmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Chembiochem. 2008 Dec 15. 9(18):2958-61. pRSF-1 CRK CRKII, p38 Add to Cart
20178pMMRSF17C-CrkII nSH3 (Homo sapiens)Bacterial ExpressionSegmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Chembiochem. 2008 Dec 15. 9(18):2958-61. pRSF-1 CRK CRKII, p38 Add to Cart
24765pHYRSF1-02NpuDnaE-IntCBacterial ExpressionSegmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Nat Protoc. 2010 . 5(3):574-87. pRSF-1b (Invitrogen) Add to Cart
31122pHYRS52Ulp1 (Saccharomyces cerevisiae)Bacterial ExpressionSegmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Chembiochem. 2008 Dec 15. 9(18):2958-61. pRsetA ULP1 YPL020C, NIB1 Add to Cart
34549pHYRSF1DnaE N-inteinBacterial ExpressionSegmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Nat Protoc. 2010 . 5(3):574-87. pRSFDuet Add to Cart
41684pSKDuet16NpuDnaE inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1 Add to Cart
41685pHYDuet183PhoRadA inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1 Add to Cart
41689pSKDuet26Tvo VMA inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1
  • Tags / Fusion Proteins
    • GB1 (N terminal on insert)
    • GB1 (C terminal on insert)
    • His-tag (N terminal on insert)
  • Add to Cart
    41694pSKDuet20MjaTFIIB inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1 Add to Cart
    41695pSKDuet21PhoCDC21-1 inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1
  • Tags / Fusion Proteins
    • GB1 (N terminal on insert)
    • GB1 (C terminal on insert)
    • His-tag (N terminal on insert)
  • Add to Cart
    41696pSKDuet22PhoCDC21-2 inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1
  • Tags / Fusion Proteins
    • His-tag (N terminal on insert)
    • GB1 (N terminal on insert)
    • GB1 (C terminal on insert)
  • Add to Cart
    41697pSKDuet23PhoPol-II inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1
  • Tags / Fusion Proteins
    • His-tag (N terminal on insert)
    • GB1 (N terminal on insert)
    • GB1 (C terminal on insert)
  • Add to Cart
    41698pSKDuet27Tac VMA inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1
  • Tags / Fusion Proteins
    • GB1 (N terminal on insert)
    • GB1 (C terminal on insert)
    • His-tag (N terminal on insert)
  • Add to Cart
    42303pHYDuet93NpuDnaEdC6Bacterial ExpressionSolution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. FEBS Lett. 2009 May 6;583(9):1451-6. doi: 10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1. pSKDuet16 Add to Cart
    42304pMHBAD14NpuDnaE C-inteinBacterial ExpressionSolution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. FEBS Lett. 2009 May 6;583(9):1451-6. doi: 10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1. pBAD Add to Cart
    42305pHYBAD94NpuDnaEC6Bacterial ExpressionSolution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. FEBS Lett. 2009 May 6;583(9):1451-6. doi: 10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1. pBAD Add to Cart
    45594pMMDuet19NpuDnaB mini inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1 Add to Cart
    45595pMKBAD28NpuDnaEC14Bacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45596pSABAD219NpuDnaEC102 inteinBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45609pSABAD331TvoVMAC48Bacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45610pSABAD332PhoRadAC46Bacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45611pHYDuet216ANpuDnaE delta variantBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSFDuet-1 Add to Cart
    45612pSABAD250NpuDnaBC39Bacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45613pSADuet760MjaTFIIB mini inteinBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSFDuet-1 Add to Cart
    45614pJORSF19PhoRadA precursorBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSF-1 Add to Cart
    45615pSABAD505GFPN-NpuDnaE_intein-GFP_CBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45616pSABAD14-750MjaTFIIBC53Bacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45617pSABAD544GFPN-NpuDnaE_AA-GFP_CBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    45618pSARSF534-3NpuDnaEC35-GFP_CBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSFDuet-1 Add to Cart
    46373pSADuet502NpuDnaB inteinBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSFDuet-1 Add to Cart
    46374pSABAD518TvoVMA intein, inactiveBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    46375pSABAD25-74TvoVMA inteinBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pBAD Add to Cart
    46376pALBRSF12NpuDnaE intein, inactiveBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSF-1 Add to Cart
    46377pHYRSF236NpuDnaE intein delta variant, inactiveBacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSF-1 Add to Cart
    46378pSEDuet40TvoVMA inteinBacterial ExpressionEvaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. FEBS Lett. 2011 Oct 10. pRSFDuet-1 Add to Cart
    46379pJDJRSF05NpuDnaE intein inactiveBacterial ExpressionNMR resonance assignment of DnaE intein from Nostoc punctiforme. Biomol NMR Assign. 2009 Jun;3(1):41-3. doi: 10.1007/s12104-008-9137-1. Epub 2009 Jan 13. pRSF-1 Add to Cart
    46832pSARSF505GFPN-NpuDnaE_intein-GFP_C (Other)Bacterial ExpressionIntermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. pRSF-1 Add to Cart
    61755pSARSF53-218NpuDnaEN35 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. NpuDnaEN35 intein pRSFDuet Add to Cart
    61756pSARSF53-756NpuDnaEN11 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. NpuDnaEN11 intein pRSFDuet Add to Cart
    61757pSARSF53-259NpuDnaB(delta283)dC39 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. NpuDnaB(delta283)dC39 intein pRSFDuet Add to Cart
    61758pSARSF53-HY100NpuDnaB(delta283)N11 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. NpuDnaB(delta283)N11 intein pRSFDuet Add to Cart
    61759pSARSF53-343TvoVMAdC48 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. TvoVMAdC48 intein pRSFDuet Add to Cart
    61760pSARSF53-143TvoVMAdC16 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. TvoVMAdC16 intein pRSFDuet Add to Cart
    61761pSARSF53-HY176TvoVMAdC7 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. TvoVMAdC7 intein pRSFDuet Add to Cart
    61813pSARSF53-110TvoVMAN11 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. TvoVMAN11 intein pRSFDuet Add to Cart
    61815pSARSF53-344PhoRadAdC46 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAdC46 intein pRSFDuet Add to Cart
    61816pSARSF53-166PhoRadAdC14 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAdC14 intein pRSFDuet Add to Cart
    61817pSARSF53-165PhoRadAdC6 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAdC6 intein pRSFDuet Add to Cart
    61818pSARSF53-459PhoRadN38 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAN38 intein pRSFDuet Add to Cart
    61819pSARSF53-455PhoRadN38 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAN13 intein pRSFDuet Add to Cart
    61823pSABAD14-HY43NpuDnaEdN11 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. NpuDnaEdN11 intein pBAD Add to Cart
    61824pSABAD14-111NpuDnaB(delta283)dN11 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. NpuDnaB(delta283)dN11 intein pBAD Add to Cart
    61825pSABAD14-142TvoVMAC16 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. TvoVMAC16 intein pBAD Add to Cart
    61826pHYBAD177TvoVMAC16 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. TvoVMAC7 intein pBAD Add to Cart
    61827pSABAD14-98TvoVMAdN11 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. TvoVMAdN11 intein pBAD Add to Cart
    61828pSABAD14-167PhoRadAC14 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAC14 intein pBAD Add to Cart
    61976pSABAD14-172PhoRadAC6 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAC6 intein pBAD Add to Cart
    61977pSABAD14-467PhoRadAdN38 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAdN38 intein pBAD Add to Cart
    61978pSABAD14-466PhoRadAdN13 intein (Other)Bacterial ExpressionStructure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. PhoRadAdN13 intein pBAD Add to Cart
    64692pLJSRSF3Bacterial ExpressionTandem SUMO fusion vectors for improving soluble protein expression and purification. Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. His-GST-Smt3-NpuDnaEN102-CBD pHYRSF53
  • Tags / Fusion Proteins
    • HisTag (N terminal on backbone)
    • GST (N terminal on insert)
    • Smt3 (N terminal on insert)
    • NpuDnaE-N102 (C terminal on backbone)
    • CBD (C terminal on backbone)
  • Add to Cart
    64693pLJSRSF7Bacterial ExpressionTandem SUMO fusion vectors for improving soluble protein expression and purification. Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. His-MBP-Smt3-NpuDnaEN102-CBD pHYRSF53
  • Tags / Fusion Proteins
    • HisTag (N terminal on backbone)
    • MBP (N terminal on insert)
    • Smt3 (N terminal on insert)
    • NpuDnaE-N102 (C terminal on backbone)
    • CBD (C terminal on backbone)
  • Add to Cart
    64694pCARSF85Bacterial ExpressionTandem SUMO fusion vectors for improving soluble protein expression and purification. Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. H6-TF-Smt3-GB1-NpuDnaEN102-CBD pHYRSF53
  • Tags / Fusion Proteins
    • HisTag (N terminal on backbone)
    • TF (N terminal on insert)
    • Smt3 (N terminal on insert)
    • NpuDnaE-N102 (C terminal on backbone)
    • CBD (C terminal on backbone)
  • Add to Cart
    64695pCARSF63Bacterial ExpressionTandem SUMO fusion vectors for improving soluble protein expression and purification. Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. H6-TRX-Smt3-GB1-NpuDnaEN102-CBD pHYRSF53
  • Tags / Fusion Proteins
    • HisTag (N terminal on backbone)
    • TRX (N terminal on insert)
    • Smt3 (N terminal on insert)
    • NpuDnaE-N102 (C terminal on backbone)
    • CBD (C terminal on backbone)
  • Add to Cart
    64696pHYRSF53Bacterial ExpressionTandem SUMO fusion vectors for improving soluble protein expression and purification. Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. His-Smt3-NpuDnaEN102-CBD pHYRSF1
  • Tags / Fusion Proteins
    • HisTag (N terminal on backbone)
    • CBD (C terminal on backbone)
    • NpuDnaE-N102 (C terminal on insert)
  • Add to Cart
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