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Izuho Hatada Lab Plasmids


The Izuho Hatada Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system. Horii T, Tamura D, Morita S, Kimura M, Hatada I Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 10.3390/ijms141019774.
pCAG-hCas9D10A (unpublished)
Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I Nat Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
51142pCAG-hCas9hCas9Mammalian Expression, CRISPRGeneration of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system. Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 10.3390/ijms141019774. Expresses hCas9 under the CAG promoter for CRISPR pEGFP-N1 Add to Cart
52101pCAG-hCas9D10AhCas9D10A (Other)Mammalian Expression pCAG-hCas9D10A (unpublished) The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter pEGFP-N1 Add to Cart
82559pPlatTET-gRNA2dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CDMammalian ExpressionTargeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Nat Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658. All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system. pCAG Add to Cart
82560pCAG-dCas9-5xPlat2AflDdCas9-5xPlat2AflDMammalian ExpressionTargeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Nat Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658. Expression vector for Cas9 peptide array (linker length: 22aa). pCAG Add to Cart
82561pCAG-scFvGCN4sfGFPTET1CDscFvGCN4sfGFPTET1CDMammalian ExpressionTargeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Nat Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658. Expression vector for antibody-sfGFP-TET1CD. pCAG Add to Cart