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Jerry Crabtree Lab Plasmids


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Title Authors Publication
NF-AT components define a family of transcription factors targeted in T-cell activation. Northrop JP, Ho SN, Chen L, Thomas DJ, Timmerman LA, Nolan GP, Admon A, Crabtree GR Nature. 1994 Jun 9. 369(6480):497-502.
Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature. Graef IA, Chen F, Chen L, Kuo A, Crabtree GR Cell. 2001 Jun 29. 105(7):863-75.
L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Graef IA, Mermelstein PG, Stankunas K, Neilson JR, Deisseroth K, Tsien RW, Crabtree GR Nature. 1999 Oct 14. 401(6754):703-8.
Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Clipstone NA, Crabtree GR Nature. 1992 Jun 25. 357(6380):695-7.
BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription. Khavari PA, Peterson CL, Tamkun JW, Mendel DB, Crabtree GR Nature. 1993 Nov 11. 366(6451):170-4.
Diversity and specialization of mammalian SWI/SNF complexes. Wang W, Xue Y, Zhou S, Kuo A, Cairns BR, Crabtree GR Genes Dev. 1996 Sep 1. 10(17):2117-30.
Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes. Chi TH, Wan M, Zhao K, Taniuchi I, Chen L, Littman DR, Crabtree GR Nature. 2002 Jul 11. 418(6894):195-9.
Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36.
An essential switch in subunit composition of a chromatin remodeling complex during neural development. Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA, Crabtree GR Neuron. 2007 Jul 19. 55(2):201-15.
Diversity and specialization of mammalian SWI/SNF complexes Wang W, Xue Y, Zhou S, Kuo A, Cairns BR, Crabtree GR. Genes Dev. 1996 Sep 1;10(17):2117-30.Click here to read
Calcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Kao SC, Wu H, Xie J, Chang CP, Ranish JA, Graef IA, Crabtree GR Science. 2009 Jan 30. 323(5914):651-4.
Calcineurin/NFAT signaling in osteoblasts regulates bone mass. Winslow MM, Pan M, Starbuck M, Gallo EM, Deng L, Karsenty G, Crabtree GR Dev Cell. 2006 Jun . 10(6):771-82.
An embryonic stem cell chromatin remodeling complex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency. Ho L, Ronan JL, Wu J, Staahl BT, Chen L, Kuo A, Lessard J, Nesvizhskii AI, Ranish J, Crabtree GR Proc Natl Acad Sci U S A. 2009 Mar 31. 106(13):5181-6.
Regulation of dendritic development by neuron-specific chromatin remodeling complexes. Wu JI, Lessard J, Olave IA, Qiu Z, Ghosh A, Graef IA, Crabtree GR Neuron. 2007 Oct 4. 56(1):94-108.
Purification and biochemical heterogeneity of the mammalian SWI-SNF complex. Wang W, Cote J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M, Workman JL, Crabtree GR EMBO J. 1996 Oct 1. 15(19):5370-82.
MicroRNA-mediated conversion of human fibroblasts to neurons. Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer C, Dolmetsch RE, Tsien RW, Crabtree GR Nature. 2011 Jul 13. doi: 10.1038/nature10323.
Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction. Beals CR, Clipstone NA, Ho SN, Crabtree GR Genes Dev. 1997 Apr 1. 11(7):824-34.
Engineering the ABA plant stress pathway for regulation of induced proximity. Liang FS, Ho WQ, Crabtree GR Sci Signal. 2011 Mar 15;4(164):rs2.
Dynamics and memory of heterochromatin in living cells. Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14.
MicroRNA-mediated switching of chromatin-remodelling complexes in neural development. Yoo AS, Staahl BT, Chen L, Crabtree GR Nature. 2009 Jul 30;460(7255):642-6. doi: 10.1038/nature08139. Epub 2009 Jun 28.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
17857pCR4 hNFATc1-EENFATc1 (Homo sapiens)Bacterial ExpressionNF-AT components define a family of transcription factors targeted in T-cell activation. Nature. 1994 Jun 9. 369(6480):497-502. pCR4-TOPO NFATC1 NF-ATC, NF-ATc1.2, NFAT2, NFATc Add to Cart
17858pCR2.1 mNFATc2NFATc2 (Mus musculus)Bacterial ExpressionNF-AT components define a family of transcription factors targeted in T-cell activation. Nature. 1994 Jun 9. 369(6480):497-502. pCR2.1-TOPO Nfatc2 AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp Add to Cart
17868pBS mNFATc3-EENFATc3 (Mus musculus)Bacterial ExpressionSignals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature. Cell. 2001 Jun 29. 105(7):863-75. pBluescript Nfatc3 C80703, D8Ertd281e, NFAT4, NFATx Add to Cart
17869pBS huNFATc4-EENFATc4 (Homo sapiens)Bacterial ExpressionL-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Nature. 1999 Oct 14. 401(6754):703-8. pBluescript NFATC4 NF-AT3, NF-ATC4, NFAT3 Add to Cart
17870pGL3-NFAT luciferase3x NFAT binding sequenceMammalian Expression, LuciferaseIdentification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Nature. 1992 Jun 25. 357(6380):695-7. 3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue pGL3 IL2 IL-2, TCGF, lymphokine Add to Cart
17871pBJ5 mCnA alphaCnA alpha (Mus musculus)Mammalian ExpressionIdentification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Nature. 1992 Jun 25. 357(6380):695-7. pBJ5 Ppp3ca 2900074D19Rik, CN, Caln, Calna, CnA Add to Cart
17872pBJ5 mCnBCnB (Mus musculus)Mammalian ExpressionIdentification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Nature. 1992 Jun 25. 357(6380):695-7. pBJ5 Ppp3r1 CaNB1, Cnb1, MCIP1 Add to Cart
17873pBJ5 hBRG1Brg (Homo sapiens)Mammalian ExpressionBRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription. Nature. 1993 Nov 11. 366(6451):170-4. pBJ5 SMARCA4 BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b Add to Cart
17874pBJ5 BRG1 DNBrg DN (Homo sapiens)Mammalian ExpressionBRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription. Nature. 1993 Nov 11. 366(6451):170-4. pBJ5 SMARCA4 BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b Add to Cart
17875pBS hBAF170BAF170 (Homo sapiens)Bacterial ExpressionDiversity and specialization of mammalian SWI/SNF complexes. Genes Dev. 1996 Sep 1. 10(17):2117-30. pBluescript SK- SMARCC2 BAF170, CRACC2, Rsc8 Add to Cart
17876pBS hBAF155BAF155 (Homo sapiens)Bacterial ExpressionDiversity and specialization of mammalian SWI/SNF complexes. Genes Dev. 1996 Sep 1. 10(17):2117-30. pBluescript SK- SMARCC1 BAF155, CRACC1, Rsc8, SRG3, SWI3 Add to Cart
17877pBS hBAF57BAF57 (Homo sapiens)Bacterial ExpressionReciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes. Nature. 2002 Jul 11. 418(6894):195-9. pBluescript SMARCE1 BAF57, CSS5 Add to Cart
17878pBS hBAF53bBAF53b (Homo sapiens)Bacterial ExpressionRapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Cell. 1998 Nov 25. 95(5):625-36. pBluescript ACTL6B ACTL6, BAF53B Add to Cart
17879pBS hBAF53aBAF53a (Homo sapiens)Bacterial ExpressionRapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Cell. 1998 Nov 25. 95(5):625-36. pBluescript ACTL6A ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K Add to Cart
17880FLAG-mBAF45a IRES GFPBAF45a (Mus musculus)Mammalian ExpressionAn essential switch in subunit composition of a chromatin remodeling complex during neural development. Neuron. 2007 Jul 19. 55(2):201-15. Beta-actin/CMV-IRES-EGFP Phf10 1810055P05Rik, AV024533 Add to Cart
17881FLAG-mBAF45b IRES GFPBAF45b (Mus musculus)Mammalian ExpressionAn essential switch in subunit composition of a chromatin remodeling complex during neural development. Neuron. 2007 Jul 19. 55(2):201-15. Beta-actin/CMV-IRES-EGFP Dpf1 Neud4 Add to Cart
17882FLAG-mBAF45c IRES GFPBAF45c (Mus musculus)Mammalian ExpressionAn essential switch in subunit composition of a chromatin remodeling complex during neural development. Neuron. 2007 Jul 19. 55(2):201-15. Beta-actin/CMV-IRES-EGFP Dpf3 CERD4, BAF45C, C78788, cer-d4, 2810403B03Rik, 6530402L11Rik Add to Cart
21034pBS-hBAF60aBAF60a (Homo sapiens)phagemidDiversity and specialization of mammalian SWI/SNF complexes Genes Dev. 1996 Sep 1;10(17):2117-30.Click here to read pBlueScript SK(-) SMARCD1 BAF60A, CRACD1, Rsc6p Add to Cart
21035pBS-hBAF60bBAF60b (Homo sapiens)phagemidDiversity and specialization of mammalian SWI/SNF complexes Genes Dev. 1996 Sep 1;10(17):2117-30.Click here to read pBlueScript SK(-) SMARCD2 BAF60B, CRACD2, PRO2451, Rsc6p Add to Cart
21036pBS-hBAF60cBAF60c (Homo sapiens)phagemidDiversity and specialization of mammalian SWI/SNF complexes Genes Dev. 1996 Sep 1;10(17):2117-30.Click here to read pBlueScript SK(-) SMARCD3 BAF60C, CRACD3, Rsc6p Add to Cart
21259MPZ(P0)promoter pGL3-TATAmyelin protein zero promoter (Mus musculus)Mammalian Expression, LuciferaseCalcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Science. 2009 Jan 30. 323(5914):651-4. pGL3 Mpz P0, Mpp, P-zero Add to Cart
21260Krox20 promoter pGL3-TATAKrox20 promoter (Mus musculus)Mammalian Expression, LuciferaseCalcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Science. 2009 Jan 30. 323(5914):651-4. pGL3 Egr2 Egr-2, Krox-20, Krox20, NGF1-B, Zfp-25, Zfp-6 Add to Cart
21261Krox20-MSE8 pGL3-TATAKrox20-Myelin Specific Enhancer (Mus musculus)Mammalian Expression, LuciferaseCalcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Science. 2009 Jan 30. 323(5914):651-4. pGL3 Egr2 Egr-2, Krox-20, Krox20, NGF1-B, Zfp-25, Zfp-6 Add to Cart
21630MPZ-intron1 pGL3-TATAmyelin protein zero intron1 (Mus musculus)Mammalian Expression, LuciferaseCalcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Science. 2009 Jan 30. 323(5914):651-4. pGL3 Mpz P0, Mpp, P-zero Add to Cart
23988pCR4-huNFATc1nucNFATc1 (Homo sapiens)cloning vectorCalcineurin/NFAT signaling in osteoblasts regulates bone mass. Dev Cell. 2006 Jun . 10(6):771-82. pCR4 NFATC1 NF-ATC, NF-ATc1.2, NFAT2, NFATc Add to Cart
24219EGFPC1-huNFATc1EE-WTNFATc1 (Homo sapiens)Mammalian ExpressionNuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction. Genes Dev. 1997 Apr 1. 11(7):824-34. pEGFPC1 NFATC1 NF-ATC, NF-ATc1.2, NFAT2, NFATc Add to Cart
24561pRRL CAGpNFlag BAF155 ires GFPBAF155 (Mus musculus)LentiviralAn embryonic stem cell chromatin remodeling complex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency. Proc Natl Acad Sci U S A. 2009 Mar 31. 106(13):5181-6. pRRL-sin18PPT CAG-ires-GFP Smarcc1 AI115498, BAF155, Rsc8, SRG3, msp3 Add to Cart
24562pRRL CAG NFlag BAF170 ires GFPBAF170 (Mus musculus)LentiviralAn embryonic stem cell chromatin remodeling complex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency. Proc Natl Acad Sci U S A. 2009 Mar 31. 106(13):5181-6. pRRL-sin18PPT CAG-ires-GFP Smarcc2 5930405J04Rik Add to Cart
24586pBS_hNFATc4nucNFATc4 (Homo sapiens)cloning vectorL-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Nature. 1999 Oct 14. 401(6754):703-8. pBS.KS+ NFATC4 NF-AT3, NF-ATC4, NFAT3 Add to Cart
25459Actin-53b-ires-EGFPBAF53b (Mus musculus)Mammalian ExpressionRegulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron. 2007 Oct 4. 56(1):94-108. Beta-actin/CMV-IRES-EGFP Actl6b Actl6, ArpNa, Baf53b Add to Cart
25460Actin-53a-ires-EGFPBAF53a (Mus musculus)Mammalian ExpressionRegulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron. 2007 Oct 4. 56(1):94-108. Beta-actin/CMV-IRES-EGFP Actl6a ARP4, Actl6, Baf53a, C79802, AI851094, 2810432C06Rik Add to Cart
25461Actin-BAF53(a+b)-ires-EGFPBAF53(a+b) (Mus musculus)Mammalian ExpressionRegulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron. 2007 Oct 4. 56(1):94-108. Beta-actin/CMV-IRES-EGFP Add to Cart
25462Actin-BAF53(b+a)-ires-EGFPBAF53(b+a) (Mus musculus)Mammalian ExpressionRegulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron. 2007 Oct 4. 56(1):94-108. Beta-actin/CMV-IRES-EGFP Add to Cart
26109pMBP_hBrg1 (J1 antibody)Brg1 (Homo sapiens)Bacterial ExpressionPurification and biochemical heterogeneity of the mammalian SWI-SNF complex. EMBO J. 1996 Oct 1. 15(19):5370-82. pMAL-c2X SMARCA4 BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b Add to Cart
31779pLemir9-124miR-9/9* and miR-124 (Mus musculus)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. pLemir Add to Cart
31780phND2-N106NeuroD2 (Homo sapiens)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. N106 NEUROD2 NDRF, bHLHa1 Add to Cart
31781phASCL1-N106achaete-scute complex homolog 1 (Homo sapiens)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. N106 ASCL1 ASH1, HASH1, MASH1, bHLHa46 Add to Cart
31782phMYT1L-N106myelin transcription factor 1-like (Homo sapiens)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. N106 MYT1L NZF1, ZC2HC4B Add to Cart
31822phND2-N174NeuroD2 (Homo sapiens)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. N174 NEUROD2 NDRF, bHLHa1 Add to Cart
31874pTight-9-124miR-9/9* and miR-124 (Mus musculus)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. pTLemiR Add to Cart
31875pTight-hND2-N106NeuroD2 (Homo sapiens)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. pTight-N106 NEUROD2 NDRF, bHLHa1 Add to Cart
31876pTight-hASCL1-N174achaete-scute complex homolog 1 (Homo sapiens)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. pTight-N174 ASCL1 ASH1, HASH1, MASH1, bHLHa46 Add to Cart
31877pTight-hMYT1L-N174myelin transcription factor 1-like (Homo sapiens)LentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. pTight-N174 MYT1L NZF1, ZC2HC4B Add to Cart
32809pLemiR-NSnon-specific hairpinLentiviralMicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. pLemiR Add to Cart
37526pShGSK3bEGSK3b (Homo sapiens)Mammalian ExpressionL-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Nature. 1999 Oct 14. 401(6754):703-8. pS GSK3B Add to Cart
38247SV-ABAactDAVP16/PYL1 (Arabidopsis thaliana), Gal4DBD/ABI* (Arabidopsis thaliana)Mammalian ExpressionEngineering the ABA plant stress pathway for regulation of induced proximity. Sci Signal. 2011 Mar 15;4(164):rs2. pUC Add to Cart
40616pUHD.NFATc1nucNFATc1nuc (Homo sapiens)Mammalian ExpressionCalcineurin/NFAT signaling in osteoblasts regulates bone mass. Dev Cell. 2006 Jun . 10(6):771-82. pUHD NFATC1 NF-ATC, NF-ATc1.2, NFAT2, NFATc Add to Cart
43968N111 nLV EF-1a-PGK-Puro Gal4-HP1a FLCbx5 (Mus musculus)LentiviralDynamics and memory of heterochromatin in living cells. Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14. N103 Add to Cart
43969N112 nLV EF-1a-PGK-Puro Gal4-HP1a CSCbx5 (Mus musculus)LentiviralDynamics and memory of heterochromatin in living cells. Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14. N103
  • Tag / Fusion Protein
    • Gal4/DBD (N terminal on insert)
  • Add to Cart
    44014N114 nLV EF-1a-Gal4-VP16-PGK-PuroGal4/DBD (1-147) (Saccharomyces cerevisiae)LentiviralDynamics and memory of heterochromatin in living cells. Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14. N103 Add to Cart
    44017N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-BlastFKBP (Homo sapiens), Cbx5 (Mus musculus)LentiviralDynamics and memory of heterochromatin in living cells. Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14. N103 Add to Cart
    44176N113 nLV EF-1a-Gal4-Stop-PGK-PuroGal4/DBD (1-147) (Saccharomyces cerevisiae)LentiviralDynamics and memory of heterochromatin in living cells. Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14. N103 Add to Cart
    44195N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-PuroCbx5 (Mus musculus)LentiviralDynamics and memory of heterochromatin in living cells. Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14. N103 Add to Cart
    44245N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-BlastFKBP (Homo sapiens)LentiviralDynamics and memory of heterochromatin in living cells. Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14. N103 Add to Cart
    59763pNSEN-d2d2-EGFP (Other), Tomato (Other)Mammalian ExpressionMicroRNA-mediated switching of chromatin-remodelling complexes in neural development. Nature. 2009 Jul 30;460(7255):642-6. doi: 10.1038/nature08139. Epub 2009 Jun 28. Expresses nuclear EGFP and Tomato in mammalian cells pUC Add to Cart