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Jordan Ward Lab Plasmids


The Jordan Ward Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
61250pJW1219sgRNA(F+E) (Synthetic)Worm Expression, CRISPRRapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Genetics. 2014 Dec 9. pii: genetics.114.172361. CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans pDD162 Add to Cart
61251pJW1259DeletionWorm Expression, CRISPRRapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Genetics. 2014 Dec 9. pii: genetics.114.172361. Cas9 plasmid with sgRNA deleted; for use in C. elegans pJW1219 Add to Cart
61252pJW1285sgRNA(F+E) targeting pha-1 (Synthetic)Worm Expression, CRISPRRapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Genetics. 2014 Dec 9. pii: genetics.114.172361. CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans pJW1219 Add to Cart
61253pJW1310U6 promoter (Caenorhabditis elegans)CRISPR ; Cloning templateRapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Genetics. 2014 Dec 9. pii: genetics.114.172361. Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion pCR-Blunt II-TOPO Add to Cart
61254pJW1311sgRNA(F+E) targeting Y61A9L9.1 (Synthetic)CRISPR ; Cloning templateRapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Genetics. 2014 Dec 9. pii: genetics.114.172361. Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion pCR-Blunt II-TOPO Add to Cart
69488pJW1328sgRNA(F+E) targeting pha-1 (Caenorhabditis elegans)Worm Expression Ward lab CRISPR plasmids (unpublished) sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans pCR-Blunt II-TOPO Add to Cart
69490pJW1354GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope (Arabidopsis thaliana)CRISPR ; template to generate cassettes for -mediated knock-inThe auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Development. 2015 Nov 9. pii: dev.129635. Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms pCR-Blunt II-TOPO Add to Cart