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Karel Svoboda Lab Plasmids


The Karel Svoboda Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Mao T, Kusefoglu D, Hooks BM, Huber D, Petreanu L, Svoboda K Neuron. 2011 Oct 6;72(1):111-23.
The subcellular organization of neocortical excitatory connections. Leopoldo Petreanu, Tianyi Mao, Scott M. Sternson & Karel Svoboda Nature. 2009 Jan 18.
A genetically encoded fluorescent sensor of ERK activity Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, Svoboda K. PNAS 2008 Dec 9;105(49):19264-9
Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K PLoS ONE. 2008 . 3(3):e1796.
Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Petreanu L, Huber D, Sobczyk A, Svoboda K Nat Neurosci. 2007 May . 10(5):663-8.
Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K Nat Neurosci. 2006 Feb . 9(2):283-91.
hSyn1-FLEX-hChR2-tdtomato (unpublished)
Spatial spread of Ras activity (unpublished)
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
pCAGGS-ChR2-Venus Channelrhodopsin2-Venus Mammalian Expression Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8. pCAGGS Add to Cart
mEGFP-HRas H-Ras (Homo sapiens) Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
mRFP-RBD(R59A)-mRFP Ras binding domain of Raf Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91. pCI Add to Cart
mEGFP-HRas S17N H-Ras (Homo sapiens) Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
mEGFP-HRas G12V H-Ras (Homo sapiens) Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
cytoplasmic EKAR (Cerulean-Venus) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity Harvey et al PNAS 2008 Dec 9;105(49):19264-9 pRK5 Add to Cart
cytoplasmic EKAR (EGFP-mRFP) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity Harvey et al PNAS 2008 Dec 9;105(49):19264-9 pCI Add to Cart
nuclear EKAR (Cerulean-Venus) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity Harvey et al PNAS 2008 Dec 9;105(49):19264-9 pRK5 Add to Cart
nuclear EKAR (EGFP-mRFP) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity Harvey et al PNAS 2008 Dec 9;105(49):19264-9 pCI Add to Cart
paGFP-HRas H-Ras (Homo sapiens) Mammalian Expression Spatial spread of Ras activity (unpublished) pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
paGFP-HRas G12V H-Ras G12V (Homo sapiens) Mammalian Expression Spatial spread of Ras activity (unpublished) pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
MARCKS(1-40)-paGFP MARCKS(1-40) (Homo sapiens) Mammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
mEGFP monomeric EGFP Mammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
paGFP photoactivatable GFP Mammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
pCAGGS-GCaMP2 GCaMP2 Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao et al PLoS ONE. 2008 . 3(3):e1796. pCAGGS Add to Cart
CMV-GCaMP2 GCaMP2 Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao et al PLoS ONE. 2008 . 3(3):e1796. pCMV Add to Cart
pCAGGS-GCaMP2-actin GCaMP2-Actin (Gallus gallus) Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao et al PLoS ONE. 2008 . 3(3):e1796. pCAGGS ACTB RCJMB04_4h19, Bact Add to Cart
pCAGGS-PSD95-GCaMP2 PSD95-GCaMP2 (Rattus norvegicus) Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao et al PLoS ONE. 2008 . 3(3):e1796. pCAGGS Dlg4 Dlgh4, PSD95, Sap90 Add to Cart
pCAGGS-hCD4-GCaMP2 hCD4-GCaMP2 (Homo sapiens) Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao et al PLoS ONE. 2008 . 3(3):e1796. pCAGGS CD4 CD4mut Add to Cart
pACAGW-ChR2-Venus-AAV Channelrhodopsin-2 Mammalian Expression ; AAV The subcellular organization of neocortical excitatory connections. Leopoldo et al Nature. 2009 Jan 18. N/A COP4 CHLREDRAFT_182032 Add to Cart
AAV-CAG-GFP eGFP adeno associate viral Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Mao et al Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
AAV-CAG-hChR2_tdTomato humanized ChR2-tdTomato adeno associate viral Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Mao et al Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
AAV-CAG-hChR2-H134R-tdTomato humanized ChR2-H134R-tdTomato adeno associate viral Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Mao et al Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
hSyn1-FLEX-hChR2-tdtomato Channelrhodopsin-2 Mammalian Expression, Adenoviral hSyn1-FLEX-hChR2-tdtomato (unpublished) pAAV (Adeno-associated Virus) Add to Cart