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Karel Svoboda Lab Plasmids


The Karel Svoboda Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Mao T, Kusefoglu D, Hooks BM, Huber D, Petreanu L, Svoboda K Neuron. 2011 Oct 6;72(1):111-23.
The subcellular organization of neocortical excitatory connections. Leopoldo Petreanu, Tianyi Mao, Scott M. Sternson & Karel Svoboda Nature. 2009 Jan 18.
A genetically encoded fluorescent sensor of ERK activity Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, Svoboda K. PNAS 2008 Dec 9;105(49):19264-9
Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K PLoS ONE. 2008 . 3(3):e1796.
Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Petreanu L, Huber D, Sobczyk A, Svoboda K Nat Neurosci. 2007 May . 10(5):663-8.
Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K Nat Neurosci. 2006 Feb . 9(2):283-91.
hSyn-FAS-DO-ReaChR-mCitrine-WPRE (unpublished)
hSyn1-FLEX-hChR2-tdtomato (unpublished)
Spatial spread of Ras activity (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
15753 pCAGGS-ChR2-Venus Channelrhodopsin2-Venus Mammalian Expression Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Nat Neurosci. 2007 May . 10(5):663-8. pCAGGS Add to Cart
18662 mEGFP-HRas H-Ras (Homo sapiens) Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18664 mRFP-RBD(R59A)-mRFP Ras binding domain of Raf Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI Add to Cart
18665 mEGFP-HRas S17N H-Ras (Homo sapiens) Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18666 mEGFP-HRas G12V H-Ras (Homo sapiens) Mammalian Expression Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18679 cytoplasmic EKAR (Cerulean-Venus) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pRK5 Add to Cart
18680 cytoplasmic EKAR (EGFP-mRFP) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pCI Add to Cart
18681 nuclear EKAR (Cerulean-Venus) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pRK5 Add to Cart
18682 nuclear EKAR (EGFP-mRFP) ERK Activity Reporter Mammalian Expression A genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pCI Add to Cart
18693 paGFP-HRas H-Ras (Homo sapiens) Mammalian Expression Spatial spread of Ras activity (unpublished) pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18694 paGFP-HRas G12V H-Ras G12V (Homo sapiens) Mammalian Expression Spatial spread of Ras activity (unpublished) pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18695 MARCKS(1-40)-paGFP MARCKS(1-40) (Homo sapiens) Mammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
18696 mEGFP monomeric EGFP Mammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
18697 paGFP photoactivatable GFP Mammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
18926 pCAGGS-GCaMP2 GCaMP2 Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS Add to Cart
18927 CMV-GCaMP2 GCaMP2 Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCMV Add to Cart
18928 pCAGGS-GCaMP2-actin GCaMP2-Actin (Gallus gallus) Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS ACTB RCJMB04_4h19, Bact Add to Cart
18931 pCAGGS-PSD95-GCaMP2 PSD95-GCaMP2 (Rattus norvegicus) Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS Dlg4 Dlgh4, PSD95, Sap90 Add to Cart
18932 pCAGGS-hCD4-GCaMP2 hCD4-GCaMP2 (Homo sapiens) Mammalian Expression Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS CD4 CD4mut Add to Cart
20071 pACAGW-ChR2-Venus-AAV Channelrhodopsin-2 Mammalian Expression ; AAV The subcellular organization of neocortical excitatory connections. Nature. 2009 Jan 18. N/A COP4 CHLREDRAFT_182032 Add to Cart
28014 AAV-CAG-GFP eGFP adeno associate viral Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
28015 AAV-CAG-hChR2_tdTomato humanized ChR2-tdTomato adeno associate viral Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
28017 AAV-CAG-hChR2-H134R-tdTomato humanized ChR2-H134R-tdTomato adeno associate viral Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
41015 hSyn1-FLEX-hChR2-tdtomato Channelrhodopsin-2 Mammalian Expression, Adenoviral hSyn1-FLEX-hChR2-tdtomato (unpublished) pAAV (Adeno-associated Virus) Add to Cart
64035 hSyn-FAS-DO-ReaChR-mCitrine-WPRE hSyn-FAS-DO-ReaChR-mCitrine-WPRE (Synthetic) Mammalian Expression, AAV hSyn-FAS-DO-ReaChR-mCitrine-WPRE (unpublished) Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs pAAV Add to Cart