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Karel Svoboda Lab Plasmids


The Karel Svoboda Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Petreanu L, Huber D, Sobczyk A, Svoboda K Nat Neurosci. 2007 May . 10(5):663-8.
Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K Nat Neurosci. 2006 Feb . 9(2):283-91.
A genetically encoded fluorescent sensor of ERK activity Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, Svoboda K. PNAS 2008 Dec 9;105(49):19264-9
Spatial spread of Ras activity (unpublished)
Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K PLoS ONE. 2008 . 3(3):e1796.
The subcellular organization of neocortical excitatory connections. Leopoldo Petreanu, Tianyi Mao, Scott M. Sternson & Karel Svoboda Nature. 2009 Jan 18.
Long-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Mao T, Kusefoglu D, Hooks BM, Huber D, Petreanu L, Svoboda K Neuron. 2011 Oct 6;72(1):111-23.
hSyn1-FLEX-hChR2-tdtomato (unpublished)
hSyn-FAS-DO-ReaChR-mCitrine-WPRE (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
15753pCAGGS-ChR2-VenusChannelrhodopsin2-VenusMammalian ExpressionChannelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Nat Neurosci. 2007 May . 10(5):663-8. pCAGGS Add to Cart
18662mEGFP-HRasH-Ras (Homo sapiens)Mammalian ExpressionSupersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18664mRFP-RBD(R59A)-mRFPRas binding domain of RafMammalian ExpressionSupersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI Add to Cart
18665mEGFP-HRas S17NH-Ras (Homo sapiens)Mammalian ExpressionSupersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18666mEGFP-HRas G12VH-Ras (Homo sapiens)Mammalian ExpressionSupersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006 Feb . 9(2):283-91. pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18679cytoplasmic EKAR (Cerulean-Venus)ERK Activity ReporterMammalian ExpressionA genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pRK5 Add to Cart
18680cytoplasmic EKAR (EGFP-mRFP)ERK Activity ReporterMammalian ExpressionA genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pCI Add to Cart
18681nuclear EKAR (Cerulean-Venus)ERK Activity ReporterMammalian ExpressionA genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pRK5 Add to Cart
18682nuclear EKAR (EGFP-mRFP)ERK Activity ReporterMammalian ExpressionA genetically encoded fluorescent sensor of ERK activity PNAS 2008 Dec 9;105(49):19264-9 pCI Add to Cart
18693paGFP-HRasH-Ras (Homo sapiens)Mammalian Expression Spatial spread of Ras activity (unpublished) pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18694paGFP-HRas G12VH-Ras G12V (Homo sapiens)Mammalian Expression Spatial spread of Ras activity (unpublished) pCI HRAS C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras Add to Cart
18695MARCKS(1-40)-paGFPMARCKS(1-40) (Homo sapiens)Mammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
18696mEGFPmonomeric EGFPMammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
18697paGFPphotoactivatable GFPMammalian Expression Spatial spread of Ras activity (unpublished) pRK5 Add to Cart
18926pCAGGS-GCaMP2GCaMP2Mammalian ExpressionCharacterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS Add to Cart
18927CMV-GCaMP2GCaMP2Mammalian ExpressionCharacterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCMV Add to Cart
18928pCAGGS-GCaMP2-actinGCaMP2-Actin (Gallus gallus)Mammalian ExpressionCharacterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS ACTB RCJMB04_4h19, Bact Add to Cart
18931pCAGGS-PSD95-GCaMP2PSD95-GCaMP2 (Rattus norvegicus)Mammalian ExpressionCharacterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS Dlg4 Dlgh4, PSD95, Sap90 Add to Cart
18932pCAGGS-hCD4-GCaMP2hCD4-GCaMP2 (Homo sapiens)Mammalian ExpressionCharacterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS ONE. 2008 . 3(3):e1796. pCAGGS CD4 CD4mut Add to Cart
20071pACAGW-ChR2-Venus-AAVChannelrhodopsin-2Mammalian Expression, AAVThe subcellular organization of neocortical excitatory connections. Nature. 2009 Jan 18. N/A COP4 CHLREDRAFT_182032 Add to Cart
28014AAV-CAG-GFPeGFPAAV ; adeno associate viralLong-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
28015AAV-CAG-hChR2_tdTomatohumanized ChR2-tdTomatoAAV ; adeno associate viralLong-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
28017AAV-CAG-hChR2-H134R-tdTomatohumanized ChR2-H134R-tdTomatoAAV ; adeno associate viralLong-Range Neuronal Circuits Underlying the Interaction between Sensory and Motor Cortex. Neuron. 2011 Oct 6;72(1):111-23. AAV2/1 Add to Cart
41015hSyn1-FLEX-hChR2-tdtomatoChannelrhodopsin-2Mammalian Expression, Adenoviral hSyn1-FLEX-hChR2-tdtomato (unpublished) pAAV (Adeno-associated Virus) Add to Cart
64035hSyn-FAS-DO-ReaChR-mCitrine-WPREhSyn-FAS-DO-ReaChR-mCitrine-WPRE (Synthetic)Mammalian Expression, AAV hSyn-FAS-DO-ReaChR-mCitrine-WPRE (unpublished) Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs pAAV Add to Cart