Kathleen Green Lab Plasmids


The Kathleen Green Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes. Nekrasova OE, Amargo EV, Smith WO, Chen J, Kreitzer GE, Green KJ J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19.
A role for caveolin-1 in desmoglein binding and desmosome dynamics. Brennan D, Peltonen S, Dowling A, Medhat W, Green KJ, Wahl JK 3rd, Del Galdo F, Mahoney MG Oncogene. 2012 Mar 29;31(13):1636-48. doi: 10.1038/onc.2011.346. Epub 2011 Aug 15.
EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2. Klessner JL, Desai BV, Amargo EV, Getsios S, Green KJ Mol Biol Cell. 2009 Jan;20(1):328-37. Epub 2008 Nov 5.
Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ J Cell Biol. 2005 Dec 19;171(6):1045-59.
p120 catenin associates with kinesin and facilitates the transport of cadherin-catenin complexes to intercellular junctions. Chen X, Kojima S, Borisy GG, Green KJ J Cell Biol. 2003 Nov 10;163(3):547-57.
Protein binding and functional characterization of plakophilin 2. Evidence for its diverse roles in desmosomes and beta -catenin signaling. Chen X, Bonne S, Hatzfeld M, van Roy F, Green KJ J Biol Chem. 2002 Mar 22;277(12):10512-22. Epub 2002 Jan 14.
Assembly of desmosomal cadherins into desmosomes is isoform dependent. Ishii K, Norvell SM, Bannon LJ, Amargo EV, Pascoe LT, Green KJ J Invest Dermatol. 2001 Jul;117(1):26-35.
Roles of plakoglobin end domains in desmosome assembly. Palka HL, Green KJ J Cell Sci. 1997 Oct;110 ( Pt 19):2359-71.
The amino-terminal domain of desmoplakin binds to plakoglobin and clusters desmosomal cadherin-plakoglobin complexes. Kowalczyk AP, Bornslaeger EA, Borgwardt JE, Palka HL, Dhaliwal AS, Corcoran CM, Denning MF, Green KJ J Cell Biol. 1997 Nov 3;139(3):773-84.
Kathleen Green Lab Plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
32227 1136- Desmoplakin-GFP Desmoplakin (Homo sapiens) Mammalian Expression Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59. pEGFP-N1 DSP DP, DPI, DPII
32228 330-Plakoglobin-Myc Plakoglobin (Homo sapiens) Mammalian Expression Palka et al J Cell Sci. 1997 Oct;110 ( Pt 19):2359-71. LK 444 JUP ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
32230 915-Plakophilin 2a-Flag Plakophilin 2a (Homo sapiens) Mammalian Expression Chen et al J Biol Chem. 2002 Mar 22;277(12):10512-22. Epub 2002 Jan 14. pFLAG-CMV-5
32231 854-Desmocollin 1a-myc Desmocollin 1a (Homo sapiens) Mammalian Expression Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35. b-actin vector
32232 726-Desmoglein2-myc Desmoglein2 (Homo sapiens) Mammalian Expression Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35. b-actin vector DSG2 ARVC10, ARVD10, CDHF5, CMD1BB, HDGC
32233 476-Desmocollin 2-myc Desmocollin 2 (Homo sapiens) Mammalian Expression Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35. b-actin vector DSC2 ARVD11, CDHF2, DG2, DGII/III, DSC3
32237 1099-N-cadherin-AAA-YFP N-cadherin (Mus musculus) Mammalian Expression Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57. pEYFP-N1
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)
  • Cdh2 CDHN, N-cadherin, Ncad
    32393 1140-Desmoplakin-flag Desmoplakin (Homo sapiens) Mammalian Expression Kathleen Green Lab Plasmids (unpublished) pFLAG-CMV-5
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)
  • DSP DP, DPI, DPII
    32398 1127-DP NTP wild type Desmoplakin (Homo sapiens) Mammalian Expression Kowalczyk et al J Cell Biol. 1997 Nov 3;139(3):773-84. pCMV DSP DP, DPI, DPII
    36989 1105-Desmoglein2-FLAG-eGFP Dsg2 (Homo sapiens) Mammalian Expression Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Epub 2008 Nov 5. pEGFP-N2 DSG2 ARVC10, ARVD10, CDHF5, CMD1BB, HDGC
    36990 Desmoglein2_tail-GST Dsg2 (Homo sapiens) Bacterial Expression Brennan et al Oncogene. 2012 Mar 29;31(13):1636-48. doi: 10.1038/onc.2011.346. Epub 2011 Aug 15. PGEX4T-1 DSG2 ARVC10, ARVD10, CDHF5, CMD1BB, HDGC
    36991 Desmoglein2-mCherry Dsg2 (Homo sapiens) Mammalian Expression Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19. pmCherry
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • DSG2 ARVC10, ARVD10, CDHF5, CMD1BB, HDGC
    36992 Desmocollin2_tail-GST Dsc2 (Homo sapiens) Bacterial Expression Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19. PGEX4T-1 DSC2 ARVD11, CDHF2, DG2, DGII/III, DSC3