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Kyle Roux Lab Plasmids


The Kyle Roux Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Roux KJ, Kim DI, Raida M, Burke B J Cell Biol. 2012 Mar 12.
An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
35700pcDNA3.1 mycBioIDBirA (Other)Mammalian ExpressionA promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J Cell Biol. 2012 Mar 12. To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag pcDNA3.1(-) Add to Cart
36047pcDNA3.1 MCS-BirA(R118G)-HABirA(R118G)-HA (Other)Mammalian ExpressionA promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J Cell Biol. 2012 Mar 12. To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag pcDNA3.1 Add to Cart
74223myc-BioID2-MCSMammalian ExpressionAn improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag pcDNA 3.1
  • Tags / Fusion Proteins
    • Myc (N terminal on backbone)
    • humanized A. aeolicus BioID2 with R40G (N terminal on backbone)
  • Add to Cart
    74224MCS-BioID2-HAMammalian ExpressionAn improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag pcDNA 3.1
  • Tags / Fusion Proteins
    • humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
    • HA (C terminal on backbone)
  • Add to Cart
    80899MCS-13X Linker-BioID2-HAMammalian ExpressionAn improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags. pcDNA3.1(-)
  • Tags / Fusion Proteins
    • humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
    • HA (C terminal on backbone)
  • Add to Cart
    80900mycBioID2-pBABE-puroMammalian Expression, RetroviralAn improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tag pBABE-puro
  • Tags / Fusion Proteins
    • humanized A. aeolicus BioID2 with R40G (N terminal on backbone)
    • myc (N terminal on backbone)
  • Add to Cart
    80901mycBioID-pBABE-puroMammalian Expression, RetroviralA promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J Cell Biol. 2012 Mar 12. To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag pBABE-puro
  • Tags / Fusion Proteins
    • BirA(R118G) (N terminal on backbone)
    • myc (N terminal on backbone)
  • Add to Cart