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Kyle Roux Lab Plasmids


The Kyle Roux Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Roux KJ, Kim DI, Raida M, Burke B J Cell Biol. 2012 Mar 12.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
35700pcDNA3.1 mycBioIDBirA (Other)Mammalian ExpressionA promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J Cell Biol. 2012 Mar 12. To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag pcDNA3.1(-) Add to Cart
36047pcDNA3.1 MCS-BirA(R118G)-HABirA(R118G)-HA (Other)Mammalian ExpressionA promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J Cell Biol. 2012 Mar 12. To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag pcDNA3.1 Add to Cart
74223myc-BioID2-MCSMammalian ExpressionAn improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag pcDNA 3.1
  • Tags / Fusion Proteins
    • Myc (N terminal on backbone)
    • humanized A. aeolicus BioID2 with R40G (N terminal on backbone)
  • Add to Cart
    74224MCS-BioID2-HAMammalian ExpressionAn improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag pcDNA 3.1
  • Tags / Fusion Proteins
    • humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
    • HA (C terminal on backbone)
  • Add to Cart