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Manuel Goncalves Lab Plasmids


The Manuel Goncalves Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
58252pAdSh.U6.gRNAS1U6.gRNAS1 cassette (Synthetic)Mammalian Expression, Adenoviral, CRISPRAdenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. U6 promoter-driven single guide RNA complementary to the human AAVS1 locus pBR322-derived pAdEasy plasmid Add to Cart
58253pAdSh.PGK.Cas9Human codon-optimized S. pyogenes Cas9 (Synthetic)Mammalian Expression, Adenoviral, CRISPRAdenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter pBR322-based pAdEasy backbone Add to Cart
58255pAdSh.U6.gRNAGFPU6.gRNAGFP cassette (Synthetic)Mammalian Expression, Adenoviral, CRISPRAdenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. U6 promoter-driven single guide RNA complementary to eGFP pBR322-derived pAdEasy plasmid Add to Cart