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Mattheos Koffas Lab Plasmids


The Mattheos Koffas Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
ePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. Xu P, Vansiri A, Bhan N, Koffas MA ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4.
Modular optimization of multi-gene pathways for fatty acids production in E. coli. Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli. Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, Englaender JA, Jones JA, Linhardt RJ, Koffas MA ACS Synth Biol. 2015 Mar 30.
ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, Schultz VL, Cress B, Linhardt RJ, Koffas MA Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, Wang Z, Koffas MA Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance DM, Hahn J, Koffas MA Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, Linhardt RJ, Koffas MA Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
"Optimization of naringenin and p-coumaric acid hydroxylation using the native E. coli hydroxylase complex, HpaBC". Jones JA, Collins SM, Lachance DM, Vernacchio VR, Koffas MA Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
49795pETM6Bacterial ExpressionePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4. ePathBrick vector for pathway engineering in E. coli. pET-Duet
  • Tag / Fusion Protein
    • S-tag (C terminal on insert)
  • Add to Cart
    49796pCDM4Bacterial ExpressionePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4. ePathBrick vector for pathway engineering in E. coli. pCDFDuet
  • Tag / Fusion Protein
    • S-tag (C terminal on insert)
  • Add to Cart
    49797pACM4Bacterial ExpressionePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4. ePathBrick vector for pathway engineering in E. coli. pACYC-Duet
  • Tag / Fusion Protein
    • S-tag (C terminal on insert)
  • Add to Cart
    49798pCOM4Bacterial ExpressionePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4. ePathBrick vector for pathway engineering in E. coli. pCOLA-Duet
  • Tag / Fusion Protein
    • S-tag (C terminal on insert)
  • Add to Cart
    49799pRSM3Bacterial ExpressionePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4. ePathBrick vector for pathway engineering in E. coli. pRSF-Duet
  • Tag / Fusion Protein
    • S-tag (C terminal on insert)
  • Add to Cart
    49808pCDM4-GLYphosphoglycerate kinase (Other), glyceraldehyde-3-phosphate dehydrogenase (Other), pyruvate dehydrogenase subunit E1 (Other), dihydrolipoamide acetyltransferase (Other), dihydrolipoamide dehydrogenase (Other)Bacterial ExpressionModular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration. pCDM4 Add to Cart
    49809pACM4-ACAACP transacylase (Other), Acetyl-CoA carboxylase (Other), acetyl-CoA carboxylase (Other), Acetyl-CoA carboxylase (Other), Acetyl-CoA carboxylase (Other)Bacterial ExpressionModular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration. pACM4 Add to Cart
    49810pETM6-FASfatty acyl-ACP thioesterase (Other), hydroxydecanoyl-ACP dehydratase (Other), b-ketoacyl-ACP synthase III (Other), 3-ketoacyl-ACP reductase (Other), enoyl-(acyl carrier protein) reductase (Other)Bacterial ExpressionModular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration pETM6 Add to Cart
    62616p148flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyImprovement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR pETM6 Add to Cart
    62617p158flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyImprovement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR pETM6 Add to Cart
    62618p168flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyImprovement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR pETM6 Add to Cart
    62619p168-759flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), Scaffold Protein encoding GBD:SH3:PDZ (1:1:4) (Synthetic)Bacterial Expression, Synthetic BiologyImprovement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4) pETM6 Add to Cart
    62620p1585588flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), dihydroflavonol 4-reductase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyImprovement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio) pETM6 Add to Cart
    65006pCRISPathBrickBacterial Expression, CRISPR, Synthetic BiologyCRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli. ACS Synth Biol. 2015 Mar 30. E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks pdCas9-Marraffini (pACYC184) Add to Cart
    65007pCRISPReporterBacterial Expression, CRISPR, Synthetic BiologyCRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli. ACS Synth Biol. 2015 Mar 30. E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site pCRISPReporter (pETM6/pET-Duet-1) Add to Cart
    65008pCRISPReporter-mCherrymCherry (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyCRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli. ACS Synth Biol. 2015 Mar 30. E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter. pCRISPReporter (pETM6/pET-Duet-1) Add to Cart
    66530pETM6-C4-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter C4 pETM6 Add to Cart
    66531pETM6-H10-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter H10 pETM6 Add to Cart
    66532pETM6-H9-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter H9 pETM6 Add to Cart
    66533pETM6-G6-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter G6 pETM6 Add to Cart
    66534pETM6-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of the Concensus T7 Promoter pETM6 Add to Cart
    66535pETM6-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter pETM6 Add to Cart
    66536pETM6-G6-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter pETM6 Add to Cart
    66537pETM6-E12-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12) pETM6 Add to Cart
    66865pETM6-M.accABCDaccA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic BiologyModular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. Encodes accA, accB, accC, accD in monocistronic configuration. pETM6 Add to Cart
    66866pETM6-P.accABCDaccA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic BiologyModular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. Encodes accA, accB, accC, accD in Pseudo-operon configuration. pETM6 Add to Cart
    71260pETM6-HpaBCHpaB (Other), HpaC (Other)Bacterial Expression, Synthetic Biology"Optimization of naringenin and p-coumaric acid hydroxylation using the native E. coli hydroxylase complex, HpaBC". Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185. Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6 pETM6 Add to Cart
    73392pETM6-At4CL-PhCHS-MsCHIAt4CL (Arabidopsis thaliana), PhCHS (Other), MsCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73393pETM6-At4CL-PhCHS-CmCHIAt4CL (Arabidopsis thaliana), PhCHS (Other), CmCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73394pETM6-At4CL-CmCHS-MsCHIAt4CL (Arabidopsis thaliana), CmCHS (Other), MsCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73395pETM6-At4CL-CmCHS-CmCHIAt4CL (Arabidopsis thaliana), CmCHS (Other), CmCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73396pETM6-Pc4CL-PhCHS-MsCHIPc4CL (Other), PhCHS (Other), MsCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73397pETM6-Pc4CL-PhCHS-CmCHIPc4CL (Other), PhCHS (Other), CmCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73398pETM6-Pc4CL-CmCHS-MsCHIPc4CL (Other), CmCHS (Other), MsCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73399pETM6-Pc4CL-CmCHS-CmCHIPc4CL (Other), CmCHS (Other), CmCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73400pETM6-Vv4CL-PhCHS-MsCHIVv4CL (Other), PhCHS (Other), MsCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73401pETM6-Vv4CL-PhCHS-CmCHIVv4CL (Other), PhCHS (Other), CmCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73402pETM6-Vv4CL-CmCHS-MsCHIVv4CL (Other), CmCHS (Synthetic), MsCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73403pETM6-Vv4CL-CmCHS-CmCHIVv4CL (Other), CmCHS (Other), CmCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73404pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)At4CL (Arabidopsis thaliana), PhCHS (Other), CmCHI (Other)Bacterial ExpressionExperimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo pETM6 Add to Cart
    73418pETM6-3F2-mCherryPromoter 3F2 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2. pETM6 Add to Cart
    73419pETM6-3H5-mCherryPromoter 3H5 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5. pETM6 Add to Cart
    73420pETM6-1B6-mCherryPromoter 1B6 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6. pETM6 Add to Cart
    73421pETM6-4F2-mCherryPromoter 4F2 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2. pETM6 Add to Cart
    73422pETM6-5F5-mCherryPromoter 5F5 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5. pETM6 Add to Cart
    73423pETM6-1D4-mCherryPromoter 1D4 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4. pETM6 Add to Cart
    73424pETM6-4A6-mCherryPromoter 4A6 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6. pETM6 Add to Cart
    73425pETM6-3A2-mCherryPromoter 3A2 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2. pETM6 Add to Cart
    73426pETM6-1E4-mCherryPromoter 1E4 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4. pETM6 Add to Cart
    73427pdCas9-M-C4Repressor C4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4. pdCas9 Add to Cart
    73428pdCas9-M-3F2Repressor C4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2. pdCas9 Add to Cart
    73429pdCas9-M-3H5Repressor 3H5 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5. pdCas9 Add to Cart
    73430pdCas9-M-1B6Repressor 1B6 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6. pdCas9 Add to Cart
    73431pdCas9-M-4F2Repressor 4F2 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2. pdCas9 Add to Cart
    73432pdCas9-M-5F5Repressor 5F5 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5. pdCas9 Add to Cart
    73433pdCas9-M-1D4Repressor 1D4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4. pdCas9 Add to Cart
    73434pdCas9-M-4A6Repressor 4A6 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6. pdCas9 Add to Cart
    73435pdCas9-M-3A2Repressor 3A2 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2. pdCas9 Add to Cart
    73436pdCas9-M-1E4Repressor 1E4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4. pdCas9 Add to Cart
    73437pdCas9-M-G6Repressor G6 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6. pdCas9 Add to Cart
    73438pETM6-G6-vioABEPG6-vioABE (Other)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6. pETM6 Add to Cart
    73439pETM6-G6-vioABE-4A6-vioCPG6-vioABE-P4A6-vioC (Other)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection. pETM6 Add to Cart
    73440pETM6-G6-vioABE-4A6-vioC-3A2-vioDPG6-vioABE-P4A6-vioC-P3A2-vioD (Other)Bacterial Expression, CRISPR, Synthetic BiologyRapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection. pETM6 Add to Cart