Melissa Harrison Lab Plasmids
The Melissa Harrison Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.
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|Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.||Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM||Genetics. 2013 May 24.|
|CRISPR dsDNA donor vectors||(unpublished)|
|ID||Plasmid||Gene/Insert||Vector Type||Publication||Hidden Extra Search Info|
|45945||pHsp70-Cas9||Codon optimized Cas9||CRISPR||Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013 May 24.||The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number. pHSS6||Add to Cart|
|45946||pU6-BbsI-chiRNA||U6-BbsI-chiRNA||Insect Expression, CRISPR||Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013 May 24.||Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. pBS-SK(+)||Add to Cart|
|46294||pBS-Hsp70-Cas9||codon optimized Cas9||Insect Expression, CRISPR||FlyCRISPR (unpublished)||A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter. pBluescript-KS(+)||Add to Cart|
|51019||pDsRed-attP||attP (Other), LoxP-3xP3-DsRed-LoxP (Synthetic)||high copy, amp resistance||CRISPR dsDNA donor vectors (unpublished)||Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP pJ204||Add to Cart|
|51026||U6-BbsI-crRNA||U6-BbsI-crRNA||Insect Expression, CRISPR||crRNA (unpublished)||Generates crRNA for use in combination with tracrRNA pBS-SK(-)||Add to Cart|