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Melissa Harrison Lab Plasmids


The Melissa Harrison Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM Genetics. 2013 May 24.
FlyCRISPR (unpublished)
crRNA (unpublished)
CRISPR dsDNA donor vectors (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
45945pHsp70-Cas9Codon optimized Cas9CRISPRGenome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013 May 24. The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number. pHSS6 Add to Cart
45946pU6-BbsI-chiRNAU6-BbsI-chiRNAInsect Expression, CRISPRGenome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013 May 24. Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. pBS-SK(+) Add to Cart
46294pBS-Hsp70-Cas9codon optimized Cas9Insect Expression, CRISPR FlyCRISPR (unpublished) A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter. pBluescript-KS(+) Add to Cart
51019pDsRed-attPattP (Other), LoxP-3xP3-DsRed-LoxP (Synthetic)high copy, amp resistance CRISPR dsDNA donor vectors (unpublished) Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP pJ204 Add to Cart
51026U6-BbsI-crRNAU6-BbsI-crRNAInsect Expression, CRISPR crRNA (unpublished) Generates crRNA for use in combination with tracrRNA pBS-SK(-) Add to Cart