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Michael Lynch Lab Plasmids


The Michael Lynch Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
SUPERSEDE (unpublished)
Lynch Lab CRISPR/Cas9 plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
65814pSMART-Ala1NADPH Alanine Dehydrogenase (Synthetic)Bacterial Expression SUPERSEDE (unpublished) NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression pSMART-HCKan (AF532107.1) ald BSU31930 Add to Cart
65815pSMART-Mev1mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase (Synthetic), mvaS: hydroxymethylglutaryl-CoA synthase (Synthetic)Bacterial Expression SUPERSEDE (unpublished) NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression pSMART-HCKan (AF532107.1) Add to Cart
65816pSMART-2,3-BDO1budA: α-acetolactate decarboxylase (Synthetic), budB: acetolactate synthase (Synthetic), budC: acetoin reductase (Synthetic)Bacterial Expression SUPERSEDE (unpublished) NADH Dependent 2,3-BDO Production Construct, Low Phosphate Dependent E. coli Expression pSMART-HCKan (AF532107.1) Add to Cart
65817pCASCADE-gltA2gltA2 guide (Synthetic)Bacterial Expression SUPERSEDE (unpublished) gltA targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
65818pCASCADE-udhAudhA guide (Synthetic)Bacterial Expression SUPERSEDE (unpublished) udhA targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
65819pCASCADE-gltA2-udhAgltA2-udhA guide array (Synthetic)Bacterial Expression SUPERSEDE (unpublished) gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction pCASCADE Add to Cart
65820pCASCADE-proDproD guide (Synthetic) SUPERSEDE (unpublished) Const. Promoter ProD targetting RNA E. coli Low Phosphate Induction pCASCADE Add to Cart
65821pCASCADEcontrol guideRNA (Synthetic)Bacterial Expression SUPERSEDE (unpublished) Control guide RNA plasmid E. coli , low phosphate induction pCASCADE Add to Cart
65822pSMART-GFPuvgfpuv (Synthetic)Bacterial Expression SUPERSEDE (unpublished) GFPuv Expression, Low Phosphate Dependent E. coli Expression pSMART-HCKan (AF532107.1) Add to Cart
65823pBT1-proD-mCherrymCherry (Synthetic)Bacterial Expression SUPERSEDE (unpublished) Const. mCherry expression , proD promoter, E. coli Expression pBT-1 Add to Cart
65824pBT1-proD-mCherry-DAS+4mCherry-DAS+4 (Synthetic)Bacterial Expression SUPERSEDE (unpublished) Const. mCherry-DAS_4 expression , proD promoter, E. coli Expression pBT-1 Add to Cart
65825pCASCADE-zwfzwf guide (Synthetic)Bacterial Expression SUPERSEDE (unpublished) zwf targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87135pSMART-ala10NADPH Alanine Dehydrogenase (Synthetic), alanine exporter (Synthetic)Bacterial Expression SUPERSEDE (unpublished) NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression pSMART-HCKan (AF532107.1) ald BSU31930 alaE b2670, ECK2664, JW2645, ygaW Add to Cart
87144pCDF-mcherry1mcherry (Synthetic)Bacterial Expression SUPERSEDE (unpublished) Constitutive E. coli mcherry Expression pCDF Add to Cart
87145pCDF-mcherry2mcherry (Synthetic)Bacterial Expression SUPERSEDE (unpublished) Constitutive E. coli mcherry Expression, with DAS4 tag pCDF Add to Cart
87146pCASCADE-gapAP1gapAP1 guide arrayBacterial Expression SUPERSEDE (unpublished) gapAP1 targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87147pCASCADE-fabI-gltA1-gltA2-gapAP1fabI-gltA1-gltA2-gapAP1 guide arrayBacterial Expression SUPERSEDE (unpublished) fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87148pCASCADE-fabI-gltA1-gltA2-udhA-zwffabI-gltA1-gltA2-udhA-zwf guide arrayBacterial Expression SUPERSEDE (unpublished) fabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87149pCASCADE-fabI-gltA1-gltA2-udhA-zwf-gapAP1fabI-gltA1-gltA2-udhA-zwf-gapAP1 guide arrayBacterial Expression SUPERSEDE (unpublished) fabI-gltA1-gltA2-udhA-zwf-gapAP1 targeting guide RNA E. coli, Low Phosphate Induction pCASCADE Add to Cart
87150pCASCADE-gltA1-gltA2-GapAP1gltA1-gltA2-gapAP1 guide arrayBacterial Expression SUPERSEDE (unpublished) gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87151pCASCADE-gltA1-gltA2-udhA-GapAP1gltA1-gltA2-udhA-GapAP1 guide arrayBacterial Expression SUPERSEDE (unpublished) gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87152pCASCADE-gltA1-gltA2-udhA-zwfgltA1-gltA2-udhA-zwf guide arrayBacterial Expression SUPERSEDE (unpublished) gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87153pCASCADE-udhA-zwfudhA-zwf guide arrayBacterial Expression SUPERSEDE (unpublished) udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87154pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1fabI-gltA1-gltA2-udhA-GapAP1 guide arrayBacterial Expression SUPERSEDE (unpublished) fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction pCASCADE Add to Cart
87172pSMART-ala11NADPH Alanine Dehydrogenase (Synthetic)Bacterial Expression SUPERSEDE (unpublished) NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression pSMART-HCKan (AF532107.1) ald BSU31930 Add to Cart
87173pSMART-ala12NADPH Alanine Dehydrogenase (Synthetic)Bacterial Expression SUPERSEDE (unpublished) NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression pSMART-HCKan (AF532107.1) ald BSU31930 Add to Cart
87174pSMART-ala13NADPH Alanine Dehydrogenase (Synthetic)Bacterial Expression SUPERSEDE (unpublished) NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression pSMART-HCKan (AF532107.1) ald BSU31930 Add to Cart
89951pKDsgRNA-malGmalG gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG. pKD46 Add to Cart
89952pKDsgRNA-arsBarsB gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB. pKD46 Add to Cart
89953pKDsgRNA-rpsLrpsL gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL. pKD46 Add to Cart
89955pKDsgRNA-trmItrmI gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI. pKD46 Add to Cart
89956pKDsgRNA-torZtorZ gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ. pKD46 Add to Cart
89957pKDsgRNA-rssArssA gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA. pKD46 Add to Cart
89958pKDsgRNA-yhhTyhhT gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT. pKD46 Add to Cart
89959pKDsgRNA-lpoBlpoB gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB. pKD46 Add to Cart
89960pKDsgRNA-NTempty gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning. pKD46 Add to Cart
89961pEM-Cas9HF1Cas9HF1 (Other)Bacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016. pCas9-CR4 Add to Cart
89962pEM-Cas9HF1-recA56Cas9HF1 (Other), recA56 (Other)Bacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair. pEM-Cas9HF1 Add to Cart
89963pKDsgRNA-rbsKrbsK gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK. pKD46 Add to Cart
89964pKDsgRNA-xylAxylA gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA. pKD46 Add to Cart
89965pKDsgRNA-srlAsrlA gRNABacterial Expression Lynch Lab CRISPR/Cas9 plasmids (unpublished) Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA. pKD46 Add to Cart