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Noboru Mizushima Lab Plasmids


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Title Authors Publication
The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Jiang P, Nishimura T, Sakamaki Y, Itakura E, Hatta T, Natsume T, Mizushima N Mol Biol Cell. 2014 Apr;25(8):1327-37. doi: 10.1091/mbc.E13-08-0447. Epub 2014 Feb 19.
Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Koyama-Honda I, Itakura E, Fujiwara TK, Mizushima N Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10.
The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Itakura E, Kishi-Itakura C, Mizushima N Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001.
Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Velikkakath AK, Nishimura T, Oita E, Ishihara N, Mizushima N Mol Biol Cell. 2012 Mar;23(5):896-909. Epub 2012 Jan 4.
Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. Yoshii SR, Kishi C, Ishihara N, Mizushima N J Biol Chem. 2011 Jun 3;286(22):19630-40. Epub 2011 Mar 18.
p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. Itakura E, Mizushima N J Cell Biol. 2011 Jan 10;192(1):17-27.
Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Itakura E, Mizushima N Autophagy. 2010 Aug;6(6):764-76.
Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. Kaizuka T, Hara T, Oshiro N, Kikkawa U, Yonezawa K, Takehana K, Iemura S, Natsume T, Mizushima N J Biol Chem. 2010 Jun 25. 285(26):20109-16.
Atg101, a novel mammalian autophagy protein interacting with Atg13. Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N Autophagy. 2009 Oct . 5(7):973-9.
Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Hosokawa N, Hara T, Kaizuka T, Kishi C, Takamura A, Miura Y, Iemura S, Natsume T, Takehana K, Yamada N, Guan JL, Oshiro N, Mizushima N Mol Biol Cell. 2009 Apr . 20(7):1981-91.
Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Itakura E, Kishi C, Inoue K, Mizushima N Mol Biol Cell. 2008 Dec . 19(12):5360-72.
FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. Hara T, Takamura A, Kishi C, Iemura S, Natsume T, Guan JL, Mizushima N J Cell Biol. 2008 May 5. 181(3):497-510.
Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. Mizushima N, Kuma A, Kobayashi Y, Yamamoto A, Matsubae M, Takao T, Natsume T, Ohsumi Y, Yoshimori T J Cell Sci. 2003 May 1. 116(Pt 9):1679-88.
Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method. Mizushima N, Yoshimori T, Ohsumi Y FEBS Lett. 2002 Dec 18. 532(3):450-4.
Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. Mizushima N, Yamamoto A, Hatano M, Kobayashi Y, Kabeya Y, Suzuki K, Tokuhisa T, Ohsumi Y, Yoshimori T J Cell Biol. 2001 Feb 19. 152(4):657-68.
A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. Mizushima N, Sugita H, Yoshimori T, Ohsumi Y J Biol Chem. 1998 Dec 18. 273(51):33889-92.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
22870pCI-neo-mAtg12autophagy-related genes 12 (Mus musculus)Mammalian ExpressionMouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method. FEBS Lett. 2002 Dec 18. 532(3):450-4. pCI-neo Atg12 4931423H11Rik, A330058M13Rik, Apg12l, Atg12l Add to Cart
22872p3xFLAG-CMV10-hAtg13autophagy-related genes 13 (Homo sapiens)Mammalian ExpressionNutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol Biol Cell. 2009 Apr . 20(7):1981-91. p3xFLAG-CMV10 ATG13 KIAA0652, PARATARG8 Add to Cart
22874pCI-neo-HA-hAtg13autophagy-related genes 13 (Homo sapiens)Mammalian ExpressionAtg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy. 2009 Oct . 5(7):973-9. pCI-neo-HA ATG13 KIAA0652, PARATARG8 Add to Cart
22875pEGFP-C1-hAtg13autophagy-related genes 13 (Homo sapiens)Mammalian ExpressionNutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol Biol Cell. 2009 Apr . 20(7):1981-91. pEGFP-C1 ATG13 KIAA0652, PARATARG8 Add to Cart
22876pEGFP-C1-hAtg101Atg13-interacting protein (Homo sapiens)Mammalian ExpressionAtg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy. 2009 Oct . 5(7):973-9. pEGFP-C1 ATG101 C12orf44 Add to Cart
22877p3xFLAG-CMV10-hAtg101Atg13-interacting protein (Homo sapiens)Mammalian ExpressionAtg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy. 2009 Oct . 5(7):973-9. p3xFLAG-CMV10 ATG101 C12orf44 Add to Cart
22948pCI-neo-hApg5-HAHomo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (Homo sapiens)Mammalian ExpressionA new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem. 1998 Dec 18. 273(51):33889-92. pCI-neo ATG5 APG5, APG5-LIKE, APG5L, ASP, hAPG5 Add to Cart
22949pCI-neo-hApg5(K130R)-HAHomo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (Homo sapiens)Mammalian ExpressionA new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem. 1998 Dec 18. 273(51):33889-92. pCI-neo ATG5 APG5, APG5-LIKE, APG5L, ASP, hAPG5 Add to Cart
22950pCI-neo-HA-hApg12autophagy related genes 12 (Homo sapiens)Mammalian ExpressionA new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem. 1998 Dec 18. 273(51):33889-92. pCI-neo ATG12 APG12, APG12L, FBR93, HAPG12 Add to Cart
22951pCI-neo-Myc-hApg12autophagy related genes 12 (Homo sapiens)Mammalian ExpressionA new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem. 1998 Dec 18. 273(51):33889-92. pCI-neo ATG12 APG12, APG12L, FBR93, HAPG12 Add to Cart
22952pEGFP-C1-hApg5Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (Homo sapiens)Mammalian ExpressionA new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem. 1998 Dec 18. 273(51):33889-92. pEGFP-C1 ATG5 APG5, APG5-LIKE, APG5L, ASP, hAPG5 Add to Cart
22956pCI-neo-mApg5autophagy related genes 5 (Mus musculus)Mammalian ExpressionDissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 2001 Feb 19. 152(4):657-68. pCI-neo Atg5 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy Add to Cart
22957pCI-neo-mApg5(K130R)autophagy related genes 5 (Mus musculus)Mammalian ExpressionDissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 2001 Feb 19. 152(4):657-68. pCI-neo Atg5 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy Add to Cart
22958pEGFP-C1-mApg5autophagy related genes 5 (Mus musculus)Mammalian ExpressionDissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 2001 Feb 19. 152(4):657-68. pEGFP-C1 Atg5 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy Add to Cart
22959pEGFP-C1-mApg5(K130R)autophagy related genes 5 (Mus musculus)Mammalian ExpressionDissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 2001 Feb 19. 152(4):657-68. pEGFP-C1 Atg5 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy Add to Cart
24290pCI-neo-HA-hUVRAGUV irradiation resistance-associated gene (Homo sapiens)Mammalian ExpressionBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pCI-neo 3xHA UVRAG DHTX, VPS38, p63 Add to Cart
24292p3xFLAG-CMV10-hUVRAGUV irradiation resistance-associated gene (Homo sapiens)Mammalian ExpressionBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. p3xFLAG-CMV-10 UVRAG DHTX, VPS38, p63 Add to Cart
24293pCI-neo-Myc-hAtg14autophagy related genes 14 (Homo sapiens)Mammalian ExpressionBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pCI-neo Myc Add to Cart
24294pCI-neo-HA-hAtg14autophagy related genes 14 (Homo sapiens)Mammalian ExpressionBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pCI-neo HA Ci3 Add to Cart
24295pEGFP-C1-hAtg14autophagy related genes 14 (Homo sapiens)Mammalian ExpressionBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pEGFP-C1 Add to Cart
24296pEGFP-C1-hUVRAGUV irradiation resistance-associated gene (Homo sapiens)Mammalian ExpressionBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pEGFP-C1 UVRAG DHTX, VPS38, p63 Add to Cart
24300p3xFLAG-CMV10-hFIP200FIP200 (Homo sapiens)Mammalian ExpressionFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. p3xFLAG-CMV-10 RB1CC1 ATG17, CC1, FIP200, PPP1R131 Add to Cart
24301p3xFLAG-CMV14-mULK1a novel protein kinase structurally related to C. elegans UNC-51 (Mus musculus)Mammalian ExpressionNutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol Biol Cell. 2009 Apr . 20(7):1981-91. p3xFLAG-CMV14 Ulk1 AU041434, Unc51.1, mKIAA0722 Add to Cart
24302p3xFLAG-CMV10-mApg16Lautophagy related genes 16L (Mus musculus)Mammalian Expression, RetroviralMouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J Cell Sci. 2003 May 1. 116(Pt 9):1679-88. p3xFLAG-CMV-10 Atg16l1 1500009K01Rik, Apg16l, Atg16l, WDR30 Add to Cart
24303pME18s-HA-hFIP200FIP200 (Homo sapiens)Mammalian ExpressionNutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol Biol Cell. 2009 Apr . 20(7):1981-91. pME18s RB1CC1 ATG17, CC1, FIP200, PPP1R131 Add to Cart
30213p3xFLAG-CMV10-hTti1Tel2 interacting protein 1 (Homo sapiens)Mammalian ExpressionTti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J Biol Chem. 2010 Jun 25. 285(26):20109-16. p3xFLAG-CMV10 TTI1 KIAA0406 Add to Cart
30214p3xFLAG-CMV10-hTel2telomere maintenance 2 (Homo sapiens)Mammalian ExpressionTti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J Biol Chem. 2010 Jun 25. 285(26):20109-16. p3xFLAG-CMV10 TELO2 CLK2, TEL2 Add to Cart
36456pEGFP-C1-hAtg2Aautophagy-related protein 2 homolog A (Homo sapiens)Mammalian ExpressionMammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol Biol Cell. 2012 Mar;23(5):896-909. Epub 2012 Jan 4. pEGFP-C1
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • ATG2A
    Add to Cart
    38191pMXs-IP-EGFP-hAtg13autophagy-related genes 13 (Homo sapiens)Mammalian Expression, RetroviralNutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol Biol Cell. 2009 Apr . 20(7):1981-91. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • ATG13 KIAA0652, PARATARG8
    Add to Cart
    38192pMXs-IP-EGFP-hFIP200FIP200 (Homo sapiens)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • RB1CC1 ATG17, CC1, FIP200, PPP1R131
    Add to Cart
    38193pMXs-IP-EGFP-ULK1a novel protein kinase structurally related to C. elegans UNC-51 (Mus musculus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • Ulk1 AU041434, Unc51.1, mKIAA0722
    Add to Cart
    38194pMXs-puro-EGFP-FAKPTK2 protein tyrosine kinase 2 (Mus musculus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-puro Ptk2 FAK, FRNK, Fadk, mKIAA4203, p125FAK Add to Cart
    38195pMXs-IP-EGFP-LC3microtubule-associated protein 1 light chain 3 beta (Rattus norvegicus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP Map1lc3b Map1lc3, Mpl3, zbs559 Add to Cart
    38196pMXs-IP-EGFP-mAtg5autophagy related genes 5 (Mus musculus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • Atg5 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy
    Add to Cart
    38197pMXs-IP-EGFP-ULK1(K46N)a novel protein kinase structurally related to C. elegans UNC-51 (Mus musculus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • Ulk1 AU041434, Unc51.1, mKIAA0722
    Add to Cart
    38198pMXs-IP-EGFP-ULK1(1-828)a novel protein kinase structurally related to C. elegans UNC-51 (Mus musculus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • Ulk1 AU041434, Unc51.1, mKIAA0722
    Add to Cart
    38201pMXs-IP-EGFP-ULK2Unc-51 like kinase 2 (Mus musculus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • Ulk2 RP23-278F12.5, A830085I22Rik, AU015340, Unc51.2, mKIAA0623
    Add to Cart
    38206pMXs-IP-EGFP-ULK2(K39T)Unc-51 like kinase 2 (Mus musculus)Mammalian Expression, RetroviralFIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J Cell Biol. 2008 May 5. 181(3):497-510. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • Ulk2 RP23-278F12.5, A830085I22Rik, AU015340, Unc51.2, mKIAA0623
    Add to Cart
    38248pMXs-IP HA-ParkinParkin (Homo sapiens)Mammalian Expression, RetroviralParkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J Biol Chem. 2011 Jun 3;286(22):19630-40. Epub 2011 Mar 18. pMXs-IP PARK2 AR-JP, LPRS2, PDJ, PRKN Add to Cart
    38249pMXs-IP GFP-Omp25Omp25 (Rattus norvegicus)Mammalian Expression, RetroviralParkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J Biol Chem. 2011 Jun 3;286(22):19630-40. Epub 2011 Mar 18. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)
  • Synj2bp Omp25
    Add to Cart
    38264pMXs-IP GFP-Atg14autophagy related genes 14 (Homo sapiens)Mammalian Expression, RetroviralBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pMXs-IP
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • ATG14 ATG14L, BARKOR, KIAA0831
    Add to Cart
    38265pMXs-puro HA-Atg14*autophagy related genes 14 (Homo sapiens)Mammalian Expression, RetroviralBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pMXs-puro
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)
  • ATG14 ATG14L, BARKOR, KIAA0831
    Add to Cart
    38266pMXs-puro HA-Atg14*dCCautophagy related genes 14 (Homo sapiens)Mammalian Expression, RetroviralBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pMXs-puro
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)
  • ATG14 ATG14L, BARKOR, KIAA0831
    Add to Cart
    38267pMXs-puro GFP-UVRAGUV radiation resistance-associated gene protein (Homo sapiens)Mammalian Expression, RetroviralBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pMXs-puro UVRAG DHTX, VPS38, p63 Add to Cart
    38268pMXs-puro Vps34-GFPPhosphatidylinositol 3-kinase catalytic subunit type 3 (Homo sapiens)Mammalian Expression, RetroviralBeclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec . 19(12):5360-72. pMXs-puro
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)
  • PIK3C3 VPS34, Vps34, hVps34
    Add to Cart
    38269pMXs-puro GFP-DFCP1Zinc finger FYVE domain-containing protein 1 (Mus musculus)Mammalian Expression, RetroviralCharacterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy. 2010 Aug;6(6):764-76. pMXs-puro Zfyve1 DFCP1, G630053K23, TAFF1, ZNFN2A1, mKIAA1589 Add to Cart
    38270pMXs-IP spGFP-ERGIC53ERGIC-53 (Homo sapiens)Mammalian Expression, RetroviralCharacterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy. 2010 Aug;6(6):764-76. pMXs-IP LMAN1 ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58 Add to Cart
    38272pMXs-IP GFP-WIPI-1WD repeat domain phosphoinositide-interacting protein 1 (Homo sapiens)Mammalian Expression, RetroviralCharacterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy. 2010 Aug;6(6):764-76. pMXs-IP WIPI1 ATG18, ATG18A, WIPI49 Add to Cart
    38273pMXs-puro HA-WIPI-1WD repeat domain phosphoinositide-interacting protein 1 (Homo sapiens)Mammalian Expression, RetroviralCharacterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy. 2010 Aug;6(6):764-76. pMXs-puro
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)
  • WIPI1 ATG18, ATG18A, WIPI49
    Add to Cart
    38274pMXs-puro GFP-b5 ERCytochrome b5 (Rattus norvegicus)Mammalian Expression, RetroviralCharacterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy. 2010 Aug;6(6):764-76. pMXs-puro
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • Cyb5a Cyb5
    Add to Cart
    38277pMXs-puro GFP-p62Sequestosome-1 (Mus musculus)Mammalian Expression, Retroviralp62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 2011 Jan 10;192(1):17-27. pMXs-puro
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • Sqstm1 A170, OSF-6, Osi, STAP, STONE14, p62
    Add to Cart
    38279pMXs-puro GFP-p62 L343ASequestosome-1 (Mus musculus)Mammalian Expression, Retroviralp62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 2011 Jan 10;192(1):17-27. pMXs-puro
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • Sqstm1 A170, OSF-6, Osi, STAP, STONE14, p62
    Add to Cart
    38280pMXs-puro GFP-p62 D337, 338, 339ASequestosome-1 (Mus musculus)Mammalian Expression, Retroviralp62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 2011 Jan 10;192(1):17-27. pMXs-puro
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • Sqstm1 A170, OSF-6, Osi, STAP, STONE14, p62
    Add to Cart
    38281pMXs-puro GFP-p62 K7A, D69ASequestosome-1 (Mus musculus)Mammalian Expression, Retroviralp62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 2011 Jan 10;192(1):17-27. pMXs-puro
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • Sqstm1 A170, OSF-6, Osi, STAP, STONE14, p62
    Add to Cart
    38282pMXs-puro GFP-p62 dCSequestosome-1 (Mus musculus)Mammalian Expression, Retroviralp62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 2011 Jan 10;192(1):17-27. pMXs-puro
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)
  • Sqstm1 A170, OSF-6, Osi, STAP, STONE14, p62
    Add to Cart
    38283pMXs-IP GFP-NBR1Next to BRCA1 gene 1 protein (Homo sapiens)Mammalian Expression, Retroviralp62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 2011 Jan 10;192(1):17-27. pMXs-IP NBR1 1A1-3B, IAI3B, M17S2, MIG19 Add to Cart
    45909pMRXIP GFP-Stx17 WTSyntaxin 17 (Homo sapiens)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 STX17 Add to Cart
    45910pMRXIP GFP-Stx17TMSyntaxin 17 (Homo sapiens)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 STX17 Add to Cart
    45911FLAG-Stx17Syntaxin 17 (Homo sapiens)Mammalian ExpressionThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. CMV10 FLAG STX17 Add to Cart
    45912FLAG-VAMP8vesicle-associated membrane protein 8 (Homo sapiens)Mammalian ExpressionThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. CMV10 FLAG VAMP8 EDB, VAMP-8 Add to Cart
    45913FLAG-VAMP7vesicle-associated membrane protein 7 (Mus musculus)Mammalian ExpressionThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. CMV10 FLAG Vamp7 Sybl1, TI-VAMP, VAMP-7 Add to Cart
    45914FLAG-Vti1bvesicle transport through interaction with t-SNAREs 1B (Homo sapiens)Mammalian ExpressionThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. CMV10 FLAG VTI1B VTI1, VTI1-LIKE, VTI1L, VTI2, v-SNARE, vti1-rp1 Add to Cart
    45915FLAG-SNAP29synaptosomal-associated protein, 29kDa (Homo sapiens)Mammalian ExpressionThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. CMV10 FLAG SNAP29 CEDNIK, SNAP-29 Add to Cart
    45918pMRXIP GFP-Stx18syntaxin 18 (Homo sapiens)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 STX18 GIG9, Ufe1 Add to Cart
    45919pMRXIP GFP-VAMP8vesicle-associated membrane protein 8 (Homo sapiens)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 VAMP8 EDB, VAMP-8 Add to Cart
    45920pMRXIP GFP-VAMP7vesicle-associated membrane protein 7 (Mus musculus)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 Vamp7 Sybl1, TI-VAMP, VAMP-7 Add to Cart
    45921pMRXIP GFP-Stx7syntaxin 7 (Homo sapiens)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 STX7 Add to Cart
    45922pMRXIP GFP-Vti1bvesicle transport through interaction with t-SNAREs 1B (Homo sapiens)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 VTI1B VTI1, VTI1-LIKE, VTI1L, VTI2, v-SNARE, vti1-rp1 Add to Cart
    45923pMRXIP GFP-SNAP29synaptosomal-associated protein, 29kDa (Homo sapiens)RetroviralThe hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. pMRXIP GFP-Ci2 SNAP29 CEDNIK, SNAP-29 Add to Cart
    58739pMRX-IP/Venus-mATG5autophagy related genes 5 (Mus musculus)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses Venus-mATG5 in mammalian cells pMRXIP Venus-Ci2 Atg5 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy Add to Cart
    58740pMRX-IP/Venus-rLC3microtubule-associated protein 1 light chain 3 beta (Rattus norvegicus)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses Venus-rLC3 in mammalian cells pMRXIP Venus-Ci2 Map1lc3b Map1lc3, Mpl3, zbs559 Add to Cart
    58742pMRX-IP/Venus-mDFCP1ZFYVE1 zinc finger, FYVE domain containing 1 (Mus musculus)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses Venus-mDFCP1 in mammalian cells pMRXIP Venus-Ci2 Zfyve1 DFCP1, G630053K23, TAFF1, ZNFN2A1, mKIAA1589 Add to Cart
    58743pMRX-IP/Venus-mULK1a novel protein kinase structurally related to C. elegans UNC-51 (Mus musculus)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses Venus-mULK1 in mammalian cells pMRXIP Venus-Ci2 Ulk1 AU041434, Unc51.1, mKIAA0722 Add to Cart
    58744pMRX-IP/SECFP-mATG5autophagy related genes 5 (Mus musculus)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses /SECFP-ATG5 in mammalian cells pMRXIP SECFP-Ci2 Atg5 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy Add to Cart
    58765pMRX-IP/SECFP-mDFCP1ZFYVE1 zinc finger, FYVE domain containing 1 (Mus musculus)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses SECFP-mDFCP1 in mammalian cells pMRXIP SECFP-Ci2 Zfyve1 DFCP1, G630053K23, TAFF1, ZNFN2A1, mKIAA1589 Add to Cart
    58785pMRX-IP/SECFP-mp62Sequestosome-1 (Mus musculus)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses SECFP-mp62 in mammalian cells pMRXIP GFP-Ci2 Add to Cart
    58994pMRX-IP/SECFP-hATG16A1Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (Homo sapiens)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses SECFP-hATG16A1 in mammalian cells pMRXIP GFP-Ci2 Add to Cart
    58995pMRX-IP/SECFP-hATG9AHomo sapiens autophagy related 9A (Homo sapiens)RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses SECFP-hATG9A in mammalian cells pMRXIP SECFP-Ci2 PPARG CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma Add to Cart
    60609pMXs-puro-RFP-ATG9AHomo sapiens autophagy related 9A (Homo sapiens)Mammalian Expression, RetroviralTemporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013 Oct;9(10):1491-9. doi: 10.4161/auto.25529. Epub 2013 Jul 10. Expresses RFP-hATG9A in mammalian cells pMXs-puro PPARG CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma Add to Cart
    67022pMXsIP GFP-VPS33AVacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (Homo sapiens)Mammalian Expression, RetroviralThe HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol Biol Cell. 2014 Apr;25(8):1327-37. doi: 10.1091/mbc.E13-08-0447. Epub 2014 Feb 19. human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector pMXs-IP VPS33A Add to Cart
    67023pMRXIP VPS16-GFPHomo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (Homo sapiens)RetroviralThe HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol Biol Cell. 2014 Apr;25(8):1327-37. doi: 10.1091/mbc.E13-08-0447. Epub 2014 Feb 19. Human VPS16 ORF was inserted into pMRXIP GFP-N vector pMRXIP GFP-N VPS16 hVPS16 Add to Cart