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Richard Huganir Lab Plasmids


The Richard Huganir Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
AMPA receptor and GEF-H1/Lfc complex regulates dendritic spine development through RhoA signaling cascade. Kang MG, Guo Y, Huganir RL Proc Natl Acad Sci U S A. 2009 Mar 3. 106(9):3549-54.
PICK1 loss of function occludes homeostatic synaptic scaling. Anggono V, Clem RL, Huganir RL J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011.
PICK1 interacts with PACSIN to regulate AMPA receptor internalization and cerebellar long-term depression. Anggono V, Koc-Schmitz Y, Widagdo J, Kormann J, Quan A, Chen CM, Robinson PJ, Choi SY, Linden DJ, Plomann M, Huganir RL Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 10.1073/pnas.1312467110. Epub 2013 Aug 5.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
21476GEF-DNrho/rac guanine nucleotide exchange factor (GEF) 2 (Rattus norvegicus)Mammalian ExpressionAMPA receptor and GEF-H1/Lfc complex regulates dendritic spine development through RhoA signaling cascade. Proc Natl Acad Sci U S A. 2009 Mar 3. 106(9):3549-54. pEGFP-C3 Arhgef2 MGC95068 Add to Cart
21477lentiviral shRNA GEF-H1rho/rac guanine nucleotide exchange factor (GEF) 2 (Rattus norvegicus)Mammalian Expression, LentiviralAMPA receptor and GEF-H1/Lfc complex regulates dendritic spine development through RhoA signaling cascade. Proc Natl Acad Sci U S A. 2009 Mar 3. 106(9):3549-54. FUGW Arhgef2 MGC95068 Add to Cart
67522pSuper-PICK1 shRNAPICK1 (Mus musculus)Mammalian Expression, RNAiPICK1 loss of function occludes homeostatic synaptic scaling. J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011. Expresses shRNA against PICK1 pSuper Pick1 Prkcabp Pick1 Prkcabp Add to Cart
72573pRK5-myc-PICK1-rescuePICK1 (Rattus norvegicus)Mammalian ExpressionPICK1 loss of function occludes homeostatic synaptic scaling. J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011. Expresses myc-PICK1 shRNA resistant cDNA pRK5 Pick1 Prkcabp Add to Cart
72575pVenus-PICK1-shRNAPICK1 (Mus musculus)Mammalian ExpressionPICK1 loss of function occludes homeostatic synaptic scaling. J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011. Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein pSuper Pick1 Prkcabp Pick1 Prkcabp Add to Cart
72576pSuper-PACSIN1-shRNAPACSIN1 (Mus musculus)Mammalian Expression, RNAiPICK1 interacts with PACSIN to regulate AMPA receptor internalization and cerebellar long-term depression. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 10.1073/pnas.1312467110. Epub 2013 Aug 5. Expresses shRNA against PACSIN1 pSuper Pacsin1 A830061D09Rik, H74, mKIAA1379, syndapin Add to Cart
72577pRK5-H1-shRNA-CMV-HA-PACSIN1PACSIN1 (Mus musculus), H1-PACSIN1 shRNA cassetteMammalian Expression, RNAiPICK1 interacts with PACSIN to regulate AMPA receptor internalization and cerebellar long-term depression. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 10.1073/pnas.1312467110. Epub 2013 Aug 5. Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct pRK5 Pacsin1 A830061D09Rik, H74, mKIAA1379, syndapin Add to Cart