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Wataru Fujii Lab Plasmids


The Wataru Fujii Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Fujii W, Kawasaki K, Sugiura K, Naito K Nucleic Acids Res. 2013 Aug 30.
Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system. Fujii W, Onuma A, Sugiura K, Naito K Biochem Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4. doi: 10.1016/j.bbrc.2014.01.141.
Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells. Yoshioka S, Fujii W, Ogawa T, Sugiura K, Naito K Sci Rep. 2015 Dec 16;5:18341. doi: 10.1038/srep18341.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
48625pCAG-T3-hCAS-pACodon optimized Cas9 (Synthetic)Mammalian Expression, CRISPREfficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res. 2013 Aug 30. Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys pCAGGS Add to Cart
51638pCAG-T3-hCasD10A-pACodon-optimized Cas9 D10A (Synthetic)Mammalian Expression, CRISPREfficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system. Biochem Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4. doi: 10.1016/j.bbrc.2014.01.141. Expresses Cas9-nickase in mammalian cells and zygotes. pCAGGS Add to Cart
59766pCAG-T3-hCASeGFP-pACAS9 (Synthetic)Mammalian ExpressionEfficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res. 2013 Aug 30. Expresses CAS9-eGFP fusion protein in mammalian cells. pCAGGS Add to Cart
64668pMCS-rybozyme-IRES-CAS9CAS9 (Synthetic), HDV ribozyme (Other)Mammalian Expression, CRISPRDevelopment of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells. Sci Rep. 2015 Dec 16;5:18341. doi: 10.1038/srep18341. Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9. pCAGGS Add to Cart