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Wensheng Wei Lab Plasmids


The Wensheng Wei Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W Nature. 2014 Apr 9. doi: 10.1038/nature13166.
Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Perez-Cordon G, Feng H, Wei W Cell Res. 2015 Feb;25(2):157-68. doi: 10.1038/cr.2014.169. Epub 2014 Dec 30.
A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Ren Q, Li C, Yuan P, Cai C, Zhang L, Luo GG, Wei W Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038/srep08865.
Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
53118pLenti-OC-IRES-BSDOCT1 (Homo sapiens), Cas9 (Other)Mammalian Expression, LentiviralHigh-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature. 2014 Apr 9. doi: 10.1038/nature13166. To create stable cell clones with high-level expression of Cas9 and OCT1 pLenti-CMV-BSD POU2F1 OCT1, OTF1, oct-1B Add to Cart
53121pLenti-sgRNA-LibMammalian Expression, Lentiviral, CRISPRHigh-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature. 2014 Apr 9. doi: 10.1038/nature13166. sgRNA expression construction in a lentiviral backbone pLL3.7, Addgene plasmid #11795 Add to Cart
65010pLenti-NIrDrtTA-MAVS(C), 2A-linked delta-TKMammalian Expression, LentiviralA Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038/srep08865. NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection pLenti-CMVMCSSVBsd Add to Cart
69037pEF6-CSPG4-myc-hisCSPG4 (Homo sapiens)Mammalian ExpressionChondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 2015 Feb;25(2):157-68. doi: 10.1038/cr.2014.169. Epub 2014 Dec 30. Expression of CSPG4 in mammalian cells pEF6-Myc-His-B CSPG4 HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2 Add to Cart
89639scaffold-linker-U6Mammalian Expression, LentiviralGenome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter. pLL3.7 Add to Cart
89640Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)12,472 pgRNAs targeting 671 human long non-coding RNAs (lncRNAs)Mammalian Expression, LentiviralGenome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. Paired sgRNA library containing 12,472 pgRNAs targeting 671 human long non-coding RNAs (lncRNAs) pLL3.7 Add to Cart