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Marx Lab FREQ-Seq Kit: High Resolution Allele Frequency Measurements
(Kit # 1000000032 )

Depositing Labs:   Christopher Marx

The FREQ-Seq collection can be used to construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing. By counting DNA sequence reads, this strategy allows for direct quantitative allele detection.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

$330 USD + shipping
Available to Academic and Nonprofits Only

Original Publication

FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012; 7(10): e47959. PubMed. Open Access Article.

Description

The FREQ-Seq collection can be used to construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing. By counting DNA sequence reads, this strategy allows for direct quantitative allele detection. The kit consists of a 48 plasmid adaptor library, with each plasmid carrying a unique barcoded Illumina-M13F bridging primer. These bridging primers can be amplified and used to generate Illumina sequencing libraries by means of a 2-step PCR-based protocol and are compatible with single-end or paired-end read flow cells.

FreqSeq Figure

Kit Documentation

Icon FREQ-Seq kit.pdf (1.3 MB)

Protocols

An abbreviated FREQ-Seq Protocol from the publication:

Icon marx-lab-freq-seq-protocol.pdf (316.9 KB)

Links

Analysis software: FREQ-Seq Website

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The FREQ-seq plasmids were a gift from Christopher Marx (Addgene kit # 1000000032)"

For your Reference section:
FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012; 7(10): e47959. PubMed. Open Access Article.

Resistance Color Key:

 
  Ampicillin  

Inventory

Well Plasmid Resistance
A / 1 pFSBC1
  Ampicillin
A / 2 pFSBC2
  Ampicillin
A / 3 pFSBC3
  Ampicillin
A / 4 pFSBC4
  Ampicillin
A / 5 pFSBC5
  Ampicillin
A / 6 pFSBC6
  Ampicillin
A / 7 pFSBC7
  Ampicillin
A / 8 pFSBC8
  Ampicillin
A / 9 pFSBC9
  Ampicillin
A / 10 pFSBC10
  Ampicillin
A / 11 pFSBC11
  Ampicillin
A / 12 pFSBC12
  Ampicillin
B / 1 pFSBC13
  Ampicillin
B / 2 pFSBC14
  Ampicillin
B / 3 pFSBC15
  Ampicillin
B / 4 pFSBC16
  Ampicillin
B / 5 pFSBC17
  Ampicillin
B / 6 pFSBC18
  Ampicillin
B / 7 pFSBC19
  Ampicillin
B / 8 pFSBC20
  Ampicillin
B / 9 pFSBC21
  Ampicillin
B / 10 pFSBC22
  Ampicillin
B / 11 pFSBC23
  Ampicillin
B / 12 pFSBC24
  Ampicillin
C / 1 pFSBC25
  Ampicillin
C / 2 pFSBC26
  Ampicillin
C / 3 pFSBC27
  Ampicillin
C / 4 pFSBC28
  Ampicillin
C / 5 pFSBC29
  Ampicillin
C / 6 pFSBC30
  Ampicillin
C / 7 pFSBC31
  Ampicillin
C / 8 pFSBC32
  Ampicillin
C / 9 pFSBC33
  Ampicillin
C / 10 pFSBC34
  Ampicillin
C / 11 pFSBC35
  Ampicillin
C / 12 pFSBC36
  Ampicillin
D / 1 pFSBC37
  Ampicillin
D / 2 pFSBC38
  Ampicillin
D / 3 pFSBC39
  Ampicillin
D / 4 pFSBC40
  Ampicillin
D / 5 pFSBC41
  Ampicillin
D / 6 pFSBC42
  Ampicillin
D / 7 pFSBC43
  Ampicillin
D / 8 pFSBC44
  Ampicillin
D / 9 pFSBC45
  Ampicillin
D / 10 pFSBC46
  Ampicillin
D / 11 pFSBC47
  Ampicillin
D / 12 pFSBC48
  Ampicillin
Data from: CALCULATED @ 2014-11-26 12:23:48.990255
Click on well to open plasmid page