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Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes.
Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH
Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
62891pT-GEM(0)Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
62893pT-GEM(1)Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
62894pT-GEM(2)Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
62896pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.
62897pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.
62898pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.
62899pBS-KS-attB2-SA(0)-T2A-Gal4Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0
62900pBS-KS-attB2-SA(1)-T2A-Gal4Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1
62901pBS-KS-attB2-SA(2)-T2A-Gal4Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2
62902pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0
62903pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1
62904pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2
62905pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0
62908pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1
62911pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2
62912pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0
62914pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1
62915pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2
62944pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0
62945pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1
62946pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2
62947pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0
62948pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1
62949pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2
62951pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.
62952pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.
62953pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.
62954pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.
62955pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.
62956pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.
62957pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.
78304pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0
78306pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1
78307pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Antibodies from Article