Random Insertion of mCherry Into VP3 Domain of Adeno-associated Virus Yields Fluorescent Capsids With no Loss of Infectivity.
Judd J, Wei F, Nguyen PQ, Tartaglia LJ, Agbandje-McKenna M, Silberg JJ, Suh J
Mol Ther Nucleic Acids. 2012 Nov 13;1:e54. doi: 10.1038/mtna.2012.46. PubMed Article
Judd J, Wei F, Nguyen PQ, Tartaglia LJ, Agbandje-McKenna M, Silberg JJ, Suh J
Mol Ther Nucleic Acids. 2012 Nov 13;1:e54. doi: 10.1038/mtna.2012.46. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65723 | pAAV2_VP1_RaPID_r1c3 | Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only |
| 65724 | pRC_RR_VP1_r1c3 | Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR |
| 65725 | pRC_RR_VP2/3 | wt VP2/VP3 only to participate in capsid assembly; no ITR's |