One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. PubMed
Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62657 | pAYC | Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer |
| 62658 | pBCW | Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer |
| 62659 | pBCV | Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer |
| 62660 | pBDT | Targeting vector for PITT landing pad knock-in into the Rosa26 locus |
| 62661 | pBDU | Targeting vector for PITT landing pad knock-in into the Rosa26 locus |
| 62662 | pBER | Donor vector for tdTomato knock-in via landing pad |
| 62663 | pBDR | Donor vector for tdTomato knock-in via landing pad |
| 62664 | pBGV | Donor vector for mCFP knock-in via landing pad |
| 62665 | pBGW | Donor vector for mCitrine knock-in via landing pad |
| 62666 | pBGX | Donor vector for Dre knock-in via landing pad |
| 62667 | pBGT | Donor vector for iRFP knock-in via landing pad |
| 62668 | pBFB | Donor vector for tdTomato knock-in via landing pad |
| 62669 | pBEZ | Donor vector for tdTomato knock-in via landing pad |
| 62670 | pBBK | Vector for PhiC31o mRNA synthesis |
| 62671 | pBGO | Donor vector for mFTH1 knock-in via landing pad |
| 62672 | pBBJ | Vector for FLPo mRNA synthesis |