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Gene circuit performance characterization and resource usage in a cell-free "breadboard".
Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
67734Pr-deGFP-MGaptOR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion
67735Pr1-deGFP-MGaptOR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion
67736Pr2-deGFP-MGaptOR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion
67738Pr1-T7RNAPOR2-OR1-Pr1 promoter expressing T7 RNAP
67739Pr2-T7RNAPOR2-OR1-P2 promoter expressing T7 RNAP
67740Pr-deCFP-MGaptOR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion
67741PT7-deGFP-MGaptT7 promoter expressing deGFP-MG aptamer fusion
67743Pr-deGFPPr promoter expressing deGFP
67744Pr-delRBS-deGFPPr promoter expressing a deGFP that is missing a ribosomal binding site

Antibodies from Article