TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.
Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. PubMed Article
Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69112 | ch-TOGKDP-GFP | For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP. |
| 69113 | pBrain-ch-TOGKDP-GFP-shch-TOG | Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells. |
| 69114 | pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG | Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells. |
| 69115 | pBrain-GFP-TACC3KDP∆678-681-shTACC3 | Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells. |
| 69116 | pBrain-GFP-TACC3KDP∆682-688-shTACC3 | Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells. |
| 69117 | pBrain-GFP-TACC3KDP(L566,567A)-shTACC3 | Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells. |