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Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei.
Alsford S, Horn D
Mol Biochem Parasitol. 2008 Sep;161(1):76-9. doi: 10.1016/j.molbiopara.2008.05.006. Epub 2008 Jun 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69242pRPa-c6MYCnTet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.
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69243pRP-c6MYCnTet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.
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69244pRPa-iSL(MCS1+2)Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
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69246pRPa-iSL(MCS3+4)Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
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Antibodies from Article