Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation.
Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T
Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69534CAG-DDdCas9VP192-T2A-EGFP-ires-puroDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
Loading...
69535pCXLE-dCas9VP192-T2A-EGFP-shP53Episomal plasmid encoding dCas9VP192 and p53 shRNA
Loading...
69536pCXLE-dCas9VP192-T2A-EGFPEpisomal plasmid encoding dCas9VP192
Loading...
69537GG-EBNA-OCT4Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter
Loading...
69538GG-destDestination plasmid for gRNA concatenation Golden Gate cloning
Loading...

Antibodies from Article