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Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.
Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
45784pBEST-p15a-PLtetO-1-UTR1-LacI-T500
45833pBADmod1-linker2-gamS
70191pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.
70194pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)Derived from 21 with silent mutations to make BsaI, BbsI compatible
70195pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector
70197pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)Promoter panel
70198pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)Promoter panel
70199pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)Promoter panel
70200pBEST-p15A-J23113-deGFP-T500 (124p)Promoter panel
70201pBEST-p15A-J23114-deGFP-T500 (125p)Promoter panel
70202pBEST-p15A-J23116-deGFP-T500 (126p)Promoter panel
70203pBEST-p15A-J23150-deGFP-T500 (127p)Promoter panel
70204pBEST-p15A-J23106-deGFP-T500 (128p)Promoter panel
70205pBEST-p15A-J23151-deGFP-T500 (129p)Promoter panel
70206pBEST-p15A-J23100-deGFP-T500 (130p)Promoter panel
70207pBEST-p15A-J23101-deGFP-T500 (131p)Promoter panel
70208pBEST-p15A-J23102-deGFP-T500 (132p)Promoter panel
70209pBEST-p15A-pNull-deGFP-T500 (133p)Promoter panel
70210P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)1 Part of 4-part switch
70211P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)1 Part of 4-part switch
70212P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)1 Part of 4-part switch
70213P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)1 Part of 4-part switch

Antibodies from Article