Cloning-free CRISPR.
Arbab M, Srinivasan S, Hashimoto T, Geijsen N, Sherwood RI
Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 10.1016/j.stemcr.2015.09.022. PubMed Article
Arbab M, Srinivasan S, Hashimoto T, Geijsen N, Sherwood RI
Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 10.1016/j.stemcr.2015.09.022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71483 | sgPal1 (p2Tol-U6-sgPal1-HygR) | Expresses palindromic sgRNA for use in self-cloning CRISPR |
| 71484 | sgPal7 (p2Tol-U6-sgPal7-HygR) | Expresses palindromic sgRNA for use in self-cloning CRISPR |
| 71485 | sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) | Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms |
| 71489 | spCas9-BlastR (pCBhCas9-BlastR) | Expresses SpCas9 and Blasticidin resistance |