A New Generation of FRET Sensors for Robust Measurement of Galphai1, Galphai2 and Galphai3 Activation Kinetics in Single Cells.
van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann C, Gadella TW Jr, Goedhart J
PLoS One. 2016 Jan 22;11(1):e0146789. doi: 10.1371/journal.pone.0146789. eCollection 2016. PubMed
van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann C, Gadella TW Jr, Goedhart J
PLoS One. 2016 Jan 22;11(1):e0146789. doi: 10.1371/journal.pone.0146789. eCollection 2016. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69620 | Galphai1-mTurquoise2 | The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2 |
| 69621 | Galphai2-mTurquoise2 | The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2 |
| 69622 | Galphai3-mTurquoise2 | The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2 |
| 69623 | Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2 | A FRET sensor for Galphai1 activation, encoded on a single plasmid. |
| 69624 | Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2 | A FRET sensor for Galphai2 activation, encoded on a single plasmid. |
| 69625 | Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2 | A FRET sensor for Galphai3 activation, encoded on a single plasmid. |
| 69626 | cpVenus-Gγ2 | The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V). |