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A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species.
Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, Umenhoffer K, Bogos B, Posfai G, Pal C
Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72677pORTMAGE-2Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
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72678pORTMAGE-3Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.
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72679pORTMAGE-4Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
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72680pORTMAGE-1Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
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Antibodies from Article