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A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
Wang C, Szaro BG
Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010. Epub 2014 Nov 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
74100pBluescriptII[attB/Ins1]"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)
74101pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015
74102pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

Antibodies from Article