CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing.
Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP
Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. PubMed Article
Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP
Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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74119 | AIO-GFP | All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing |
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74120 | AIO-mCherry | All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing |
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74630 | AIO-Puro | All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing |
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