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CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing.
Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP
Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74119AIO-GFPAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing
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74120AIO-mCherryAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing
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74630AIO-PuroAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
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Antibodies from Article