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CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3.
Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 2016 Feb 22. PubMed Article

Plasmids from Article

ID Plasmid Purpose
78804pcDNA3-HA-RIPK3To overexpress RIPK3 in Mammalian Cells
78805pcDNA3-HA-mRipk3To overexpress mRipk3 in Mammalian Cells
78806pcDNA3-HA-RIPK3 S199ATo overexpress RIPK3 S199A in Mammalian Cells
78807pcDNA3-HA-RIPK3 K55RTo overexpress RIPK3 K55R in Mammalian Cells
78808pcDNA3-HA-RIPK3 K89RTo overexpress -RIPK3 K89R in Mammalian Cells
78809pcDNA3-HA-RIPK3 K363RTo overexpress RIPK3 K363R in Mammalian Cell
78810pcDNA3-HA-RIPK3 K501RTo overexpress RIPK3 K501R in Mammalian Cells
78811pcDNA3-HA-RIPK3 2KR(55,363)To overexpress RIPK3 2KR(55,363) in Mammalian Cells
78812pcDNA3-HA-RIPK3 2KR(89,501)To overexpress RIPK3 2KR(89,501) in Mammalian Cells
78813pcDNA3-HA-RIPK3 4KRTo overexpress RIPK3 4KR in Mammalian Cell
78814pcDNA3-HA-RIPK3 V460PTo overexpress RIPK3 V460P in Mammalian Cells
78815pcDNA3-FLAG-RIPK3To overexpress RIPK3 in Mammalian Cells
78817pcDNA3-FLAG-RIPK3 K50ATo overexpress RIPK3 K50A in Mammalian Cells
78818pcDNA3-FLAG-RIPK3 S199ATo overexpress RIPK3 S199A in Mammalian Cells
78819pcDNA3-FLAG-RIPK3 2KR(55,363)To overexpress RIPK3 2KR(55,363) in Mammalian Cells
78820pcDNA3-FLAG-RIPK3 2KR(89,501)To overexpress RIPK3 2KR(89,501) in Mammalian Cells
78821pcDNA3-FLAG-RIPK3 4KRTo overexpress RIPK3 4KR in Mammalian Cells
78822pEGFP-N2-RIPK3To detect localization of RIPK3 protein in live mammalian cells
78823pEGFP-N2-RIPK3 2KR(55,363)To detect localization of RIPK3 2KR(55,363) protein in live mammalian cells
78824pEGFP-N2-RIPK3 2KR(89,501)To detect localization of RIPK3 2KR(89,501) protein in live mammalian cells
78825pEGFP-N2-RIPK3 4KRTo detect localization of RIPK3 4KR protein in live mammalian cells
78826pEGFP-N2-RIPK3 V460PTo detect localization of RIPK3 V460P protein in live mammalian cells
78827pGEX-4T-1-RIPK3To purify GST-tagged recombinant RIPK3 protein
78828pGEX-4T-1-RIPK3 4KRTo purify GST-tagged recombinant RIPK3 4KR protein
78829pBabe-puro-RIPK3retroviral expression of RIPK3
78830pBabe-puro-mRipk3retroviral expression of mRIPK3
78831pBabe-puro-RIPK3 2KR(55,363)retroviral expression of RIPK3 2KR(55,363)
78832pBabe-puro-RIPK3 2KR(89,501)retroviral expression of RIPK3 2KR(89,501)
78833pBabe-puro-RIPK3 4KRretroviral expression of RIPK3 4KR
78834pcDNA3-HA-RIPK1To overexpress RIPK1 in Mammalian Cell
78835pcDNA3-HA-RIPK1 K115RTo overexpress RIPK1 K115R in Mammalian Cells
78836pcDNA3-HA-RIPK1 K163RTo overexpress RIPK1 K163R in Mammalian Cells
78837pcDNA3-HA-RIPK1 K316RTo overexpress RIPK1 K316R in Mammalian Cells
78838pcDNA3-HA-RIPK1 K571RTo overexpress RIPK1 K571R in Mammalian Cells
78839pcDNA3-HA-RIPK1 K604RTo overexpress RIPK1 K604R in Mammalian Cells
78840pcDNA3-HA-RIPK1 K627RTo overexpress RIPK1 K627R in Mammalian Cells
78841pcDNA3-HA-RIPK1 3KRTo overexpress RIPK1 3KR in Mammalian Cells
78842pcDNA3-FLAG-RIPK1To overexpress RIPK1 in Mammalian Cells
78843pcDNA3-FLAG-RIPK1 K115RTo overexpress RIPK1 K115R in Mammalian Cells
78844pcDNA3-FLAG-RIPK1 K163RTo overexpress RIPK1 K163R in Mammalian Cells
78845pcDNA3-FLAG-RIPK1 K316RTo overexpress RIPK1 K316R in Mammalian Cells
78846pEGFP-N2-RIPK1To detect localization of RIPK1 protein in live mammalian cells
78847pEGFP-N2-RIPK1 3KRTo detect localization of RIPK1 3KR protein in live mammalian cells
78848pEGFP-N2-RIPK1 I539DTo detect localization of RIPK1 I539D protein in live mammalian cells

Antibodies from Article