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Programmable RNA-binding protein composed of repeats of a single modular unit.
Adamala KP, Martin-Alarcon DA, Boyden ES
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 10.1073/pnas.1519368113. Epub 2016 Apr 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87319PumAPumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)
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87320PumGPumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the Pumby for G)
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87321PumCPumHD w/each unit mutated to bind C (as in Fig. 1C; unit 6 is the Pumby for C)
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87322PumUPumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the Pumby for U)
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97189[UBC][EIF4e][GG cloning]EIF4e plasmid with Pum cloning site
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97190[UBC][firefly luciferase][target cloning][renilla luciferase]Reporter for translation activation via Pum.
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97191[GG][Pin]Pin nuclease domain with Pum cloning site
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97192[UBC][NRE Pum][eGFP]The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.
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97193[Bla][IRES][GFP]Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.
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97194[GFP][IRES][Bla]Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.
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Antibodies from Article