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Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI
Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79029pDONR221-OSF-TRIM5-21R(1-300)Gateway entry vector for cloning and propagation in DH5alpha
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79030pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79031pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79032pFastBac1-OSF-TRIM5alpha(chimpanzee)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79033pFastBac1-OSF-TRIM5alpha(rhesus)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79034pFastBac1-OSF-TRIM5alpha(human)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79035pFastBac1-OSF-TRIMCyp (owl monkey)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79036pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79037pFastBac1-OSF-TRIM5alpha(human)(R332P)Baculoviral entry vector to produce Bac-to-bac baculoviruses
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79038pFastBac1-Kozak-rhTRIM5alpha-PFOSBaculoviral entry vector to produce Bac-to-bac baculoviruses
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79039pET3a-OSF-CypABacterial vector for cloning and propagation in DH5alpha
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79045pET11a-CA(A14C, E45C)Bacterial vector for cloning and propagation in DH5alpha
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79046pET11a-CA(A14C, E45C, A92E)Bacterial vector for cloning and propagation in DH5alpha
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79047pCMV-deltaR8.2(A14C,E45C)mammalian expression, lentiviral packaging
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79048pCMV-deltaR8.2(A14C,E45C,A92E)mammalian expression, lentiviral packaging
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79059CSII-IDR2-rhesusTRIM5-FOSExpress rhesus TRIM5 in mammalian cells
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79063pDONR221-OSF-TRIM5-21RGateway entry vector for cloning and propagation in DH5alpha
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79064CSII-IDR2-AGMpygTRIM5-FOSExpress African green monkey pygerythrus TRIM5alpha in mammalan cells
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79065CSII-IDR2-chimpTRIM5-FOSExpress chimpanzee TRIM5alpha in mammalan cells
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79066CSII-IDR2-humanTRIM5-FOSExpress human TRIM5alpha in mammalan cells
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79067CSII-IDR2-owlTRIMCyp-FOSExpress owl monkey TRIMCyp in mammalan cells
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79068CSII-IDR2-owlTRIMCyp-FOS K283D Q287DExpress owl monkey TRIMCyp K283D, Q287D in mammalan cells
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Antibodies from Article